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Results The recombinant MPT 64 protein existed in inclusion bodies of E.coli.and amounted to 20%- 30% of totle cell protein.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

These results indicated that the recombinant PEPCK might be one of the suitable antigens for serodiagnosis of tuberculosis.

结核杆菌PEPCK具有较好的免疫原性和抗原性,有可能作为结核病血清学诊断的一组抗原之一。

In order to evaluate the potential of this immunodominant peptide antigen in serodiagnosis of TB, we have undertaken cloning and expression of a large number of esat-6p1 genes in Escherichia coli and purification products.

本实验研究目的是通过构建结核菌ESAT-6蛋白免疫优势段P1的重组多表达载体,在大肠杆菌 BL21(DE3)中进行诱导表达,并对表达产物进行纯化和复性,以获得大量ESAT-6P1抗原,同时对重组多免疫反应性进行鉴定评价,为探索重组多在结核病血清学诊断的应用奠定前期实验基础。

The serum samples were detected by Indirect ELISA and Indirect hemagglutination test as reference method to assess the value of the recombinant protein in serodiagnosis.

结论1、大量表达并纯化了重组蛋白SjRPS4,并证实其具有良好的免疫反应性,能与日本血吸虫病阳性血清特异性结合,可应用于日本血吸虫感染血清学诊断。2、成功建立了以重组蛋白SjRPS4为包被抗原的间接ELISA法,在日本血吸虫病的免疫诊断中具有良好的应用价值。

The protein component with 23 kDa in T. spiralis ESA is the specific antigen of T. spiralis muscle larvae and it could be applied to the serodiagnosis and seroepidemiological survey of trichinellosis.

旋毛虫肌幼虫ES抗原中的23kDa蛋白组分为旋毛虫肌幼虫的特异性抗原,可用于旋毛虫病的血清学诊断及血清流行病学调查。

Our results indicate that though Paragonimus skrjabini and Clonorchis sinensis were independent species, similar genes exist because of their close genetic relation. It should be further investigated whether these similar genes express common antigens resulting in cross-reaction in serodiagnosis.

RAPD结果显示斯氏肺吸虫和华支睾吸虫为2种独立的吸虫虫种,由于遗传的亲缘关系存在相同的基因,这些相同基因是否表达共同的抗原组分,并导致血清学诊断的交叉反应尚待进一步研究。

The recombinant protein of purification and renaturation may be a potential candidate to be a serodiagnostic reagent.

具有天然结构和高纯度的重组融合蛋白有可能成为有效的结核病血清学诊断的候选抗原。

Objective To evaluate the Cell Wall antigen and recombinant 38Kd protein (CW and Rtpa38) as serodiagnostic reagents.

目的 探讨结核杆菌CW抗原和rTPA38蛋白用于结核病血清学诊断的价值。

The fused dominant peptide antigen 38 kD-ESAT6-CFP10 might serve as a serodiagnostic candidate antigen, and the double antigen sandwich ELISA using the fused antigen might be used to detect the tuberculosis and improve the specificity and sensitivity of diagnostic reagent.

该种抗原优势肽段融合抗原有望成为结核病血清学诊断的重要候选抗原,并且双抗原夹心ELISA方法有助于提高检测的特异性和敏感性。

Objective: Establish mucins' protein chip and test the serum levels of mucinl,...

目的:应用粘蛋白抗体制备粘蛋白检测用蛋白芯片,并对胃肠道癌症患者血清进行粘蛋白MUC1、MUC2、MUC5AC及CEA的检测,与健康人群进行对照,以揭示粘蛋白在胃肠道癌症患者血清中的表达规律及可能预示的临床意义,探讨该芯片用于胃肠道癌症筛查和血清学诊断的可行性。

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