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The allergens with molecular mass of 21kD and 11kD were identified by Western-blotting. The 21kD and 11kD allergens of cashew nut were purified by ion exchange chromatography.

结果 腰果粗提液SDS-PAGE显示有10条蛋白条带,Western-blotting显示对腰果过敏患者的阳性混合血清能与其中2个蛋白条带起反应,分子量分别为21kD和11kD。

To evaluate the effect of PRRSV on humoral and cellular immune responses,threeweek old SPF piglets and conventional piglets with antibody to PRRSV were inoculated nasally with PRRSV BJ 4 strain,and immunized intramuscularly with hog cholera vaccine 48 hours postinoculation.

对 2 0日龄 SPF猪和 2 0日龄猪繁殖与呼吸综合征血清阳性猪,人工感染猪繁殖与呼吸综合征病毒北京分离株( BJ-4 )后 4 8h接种猪瘟疫苗,利用 ELISA方法检测仔猪针对 PRRSV和猪瘟疫苗的体液免疫,利用 MTS法检测仔猪外周血单核细胞对有丝分裂原 Con A的刺激反应。

Western blot assay indicated that the crude extract reacted with serum obtained from sixteen Amaranthus spinosus pollen-allergic subjects and contained four allergen bands in total, and that the 78 kD and 40 kD bands were the major allergenic components.

Western blotting结果表明,16份刺苋花粉过敏患者的血清全部呈阳性反应,浸出液中共有4条致敏条带,其中主要致敏蛋白的分子量是78 kD和40 kD。

All major neuropil regions are labeled by 5-HT antiserum except the optic lobes, antennal lobes and calyces of mushroom bodies, which are almost devoid of 5-HT immunoreactivity.

除常规的免疫阳性神经元(仅细胞质被标记的神经元和整个胞体都被标记的神经元)外,我们在前脑中部及触角叶内侧还发现了一些只有细胞核对 5-HT 抗血清发生反应的特殊神经元。

Nucleoprotein is also a major immunogenetic antigen for SARS; however, several tests have shown that N protein had the reactivity with other coronavirus positive sera. It is therefore an advantage of S protein as recombinant target antigen for SARS serum diagnosis compared with recombinant N protein. Moreover, S protein has been shown to be the major antigenic determinant that induces neutralizing antibodies and protective immunity to SARS-CoV, so it is also important in designing SARS genetic engineering vaccine.

SARS-CoV S 蛋白由1255 个氨基酸组成,体外表达如此大的蛋白很困难,而且表达蛋白的可溶性不高;其次,实验已经证实,全长S 蛋白含有大量无关的表位,致使表达的全长S蛋白对SARS 阳性血清的检出率低于S 蛋白片段;另外作为疫苗研究,S 蛋白上大量无关表位作为异体蛋白进入体内,会引发机体的过度免疫反应,这也是SARS-CoV 可能的致病机理之一。

AIM: To detect the stability of the recombinant adenovirus plasmid containing major outer membrane protein gene from Chlamydia psittaci in HEK293 cells, to evaluate whether cross reactivity exists or not between recombinant adenovirus and positive serum of Egg Drop Symdrome, and to evaluate whether SPF chicks vaccinated with the recombinant adenovirus were protected or not when challenged with virulent CpL strain.

目的: 检测重组腺病毒质粒在HEK293细胞中的稳定性;重组腺病毒是否与减蛋综合征阳性血清发生中和反应,以及用重组腺病毒免疫SPF小鸡是否产生保护。

Meanwhile many cases were diagnosed to be sero positive of anti dengue virus. Dengue I virus were also isolated from five cases. RT PCR test also indicated 4 out of 8 samples contained dengue I virus, which was the same type of dengue virus found in the epidemic areas in Guangzhou at the same time of outbreak.

进一步的调查在病例的血标本中分离出5株登革I型病毒,同时用RT—PCR的方法从8份标本中检出4份登革I型病毒阳性,与同期广州市登革热流行的病毒型别一致;取患者的恢复期血清作肥达氏反应,结果抗&O&、&H&的滴度与第一份血相比,均显著下降。

Seronegative Arthritis : These are a class of arthritis disorders that manifest symptoms similar to those of rheumatoid arthritis, but the patient does not test positive for that condition, or osteoarthritis.

血清关节炎:这是一类关节炎疾病表现的症状类似,以类风湿关节炎,但病人并不测试呈阳性反应这一条件,或骨性关节炎。

The model of ECV-304 cell oxidative stress injury was established by hydrogen peroxide (H2O2).Then EPZ-contained blood serum was taken as experimental drug. The adherence of monocytes to endothelial cell were measured by method of rose Bengal. The total RNA of cells was extracted. The intercellular adhesion molecule-1 (ICAM-1),vascular cell adhesion molecule-1 (VCAM-1) and MCP-1 mRNA expression in cells were detected by RT-PCR.MCP-1 protein expression were detected by ELISA.

采用过氧化氢建立离体培养的血管内皮细胞(ECV-304)氧化应激损伤模型,取健康大鼠每日灌服不同剂量的鲜姜有效部位(200,400,800 mg·kg-1)或阳性对照药洛伐他汀(40 mg·kg-1),取含药血清作为受试药物,用孟加拉玫红染色法测定单核细胞与内皮细胞的黏附力;逆转录聚合酶链反应测定细胞内细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子(VCAM-1)以及单核细胞趋化蛋白(MCP-1)的mRNA表达水平;酶联免疫吸附法测定MCP-1蛋白表达水平。

In this case, an absorbance reading greater than 0.1 above background indicates a positive reaction; the serotype of FMD virus can also be identified.

在这种情况下,吸光度值比背景大O.1即判为阳性反应。FMD病毒的血清型也能鉴定出来。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?