血清反应阳性的

A suspected new virus in ailanthus mosaic and/or ringspots samples was foundserologically close related to WMV.

对表现花叶和环斑症状的臭椿样本进行病毒检测，发现与WMV抗血清呈阳性反应的样本高达95%。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

Western-blotting confirms, but the shirt-sleeve albumen that dissolve conveys can have good immune reactivity with CCK-8 electropositive serum.

Western-blotting证实，可溶表达的融合蛋白能和CCK-8阳性血清具有良好的免疫反应性。

Circulating antigen was detected in 29 out of 70 cases with paragonimiasis with a sensitivity of 41.5%. The rate of cross reaction in cases with clonorchiasis sinensis and schistosomiasis was 25%（5/20） and 20%（4/20）, respectively, and it was negative in 60 casess with other parasitic infections and healthy subjects, with an overall specificity of 93.6%.

用CAg-dot-ELISA检测70份卫氏并殖吸虫病临床诊断患者血清，阳性29份，敏感性为41.5%（29/70），与华支睾吸虫病和日本血吸虫病患者血清分别有25%（5/20）和20%（4/20）的交叉反应，与其他寄生虫感染者血清和健康人血清（60份）均为阴性，特异性为93.6%。

ELISA method was used for comparison. Results The positive rate of DIGFA in testing the serum from 50 infected rats was 96.0%(48/50). The negative rate of DIGFA in healthy rats was 100%(50/50). Cross reaction with Enteroclysis (13 samples) and Tapeworm (11 sqamples) was not observed. The cross reaction rate in 18 Strongyloidiasis rats was 5.6%. The sensitivity and specificity of DIGFA is comparable with ELISA (96.7%).

结果 用DIGFA检测广州管圆线虫实验感染鼠血清50份，其阳性检出率为96.0%，50份正常鼠血清的阴性符合率达100%，DIGFA分别检测蛲虫阳性鼠血清13份，绦虫阳性鼠血清11份和粪类圆线虫阳性鼠血清18份，前二者均为阴性，后者交叉反应率为5.6%；DIGFA与ELISA平行检测30份广州管圆线虫阳性鼠血清，两法符合率达96.7%。

They couldn't be detected by the human sera against cysticercosis, and the reaction between GST-B? and the sera from pigs against oncosphere suggests that 62 be of immunogenicity.

免疫学分析(Western-blot)结果表明，二者均与囊虫患者的阳性血清不反应或反应很弱；B_2能与猪抗六钩蚴血清反应，说明B_2可能具有免疫活性。

Owing to the variability of HVR1, the method to check anti-HVR1 antibodies need to cloning and expressing the gene of HVR1 of every sera and that poses some problem. For example, it needs lots of time and money, and the sample must come from viremic patients. It is urgent to acquire a broadly cross-reactivity HVR1 antigen to check the antiHVR1 Ab in the sera of HCV infected patients by ELISA.

由于HVR1的高变性，因此要想测定血清中抗HVR1水平，通常要克隆表达待测血清中的HVR1基因，但该方法时间长、费用高，并仅对HCV、RNA阳性血清适用，所以急需一种具有极高交叉反应性的HVR1抗原，才可通过ELISA对病人的HVR1抗体进行检测。

The minor core protein s C-encoding gene of Muscovy duck reovirus was cloned into theprokaryotic expression vector pET32a. The recombinant plasmid pET32a-s C was amplified andextracted after being transformed into E.coli DH5a competent cells. Restriction analysis withEcoRⅠand SacⅠand sequences analysis indicated that the recombinant plasmid was inserted withcorrect open reading frame. The fusion protein about 50 ku was produced after induction with 0.15mmol/L IPTG of E.coli competent cells transformed with pET32a-sC. The SDS-PAGE andWestern-bloting test indicated that the fusion protein reacted with the convalescents sera of duckinfected with Muscovy duck reovirus. The indirect ELISA method was developed by using thepurified fusion s C protein as coating antigen. The optimal concentration of s C was 5μg/ml, thedilution of serum sample was 1:40; The results showed that preparation of an ELISA by using sCas coating antigen in detecting 50 field duck sera in comparison with the AGIP were more sensitiveand specific than agar gel immuno-diffusion AGIP test. The results suggest that presence ofantibody against viral protein sC in duck may be a good indicator by the sC-ELISA for detectionof duck infection with reovirus.

同时，本研究将编码外壳蛋白σC的基因克隆于原核表达载体pET32a上，经过EcoRⅠ和SacⅠ双酶切鉴定和序列分析后，得到阳性重组质粒pET32a-σC；将阳性重组质粒pET32a-σC转化到大肠杆菌BL-21感受态细胞中进行诱导表达，经SDS-PAGE和Western-blbtting检测分析，融合表达的蛋白能够与番鸭呼肠孤病毒感染的康复鸭血清发生特异性反应；将融合表达的蛋白纯化后作为包被抗原，建立了检测鸭血清中呼肠孤病毒抗体的间接酶联免疫吸附试验检测方法，此方法中抗原的最佳包被浓度为5μg／ml、标准阳性血清的最适稀释倍数为1：40倍，用此方法对50份鸭血清样品进行检测，并与琼脂糖凝胶扩散试验检测抗体的法相比较，证明此ELISA方法具有良好的特异性和敏感性，本研究为今后鸭呼肠孤病毒诊断试剂盒的研制奠定了基础。

This test is specific to dengue virus serotype 1 without cross reaction to the other three serotypes.

检测16例临床确诊DEN1感染病人急性期血清样品，15例呈特异的抗原反应阳性。

Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.

对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛，初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。