血清反应

Effect of amphetamine and phencyclidine on DNA binding activities of serum response and dyad symmetry elements .

影响安非他明和苯环的DNA结合活动的血清反应和对子对称要素。

The levels of schistosome circulating cathodic antigen in serum and urine were detected parallelly by McAb-Dot-ELISA. In 81 acute cases, the positive rates in serum and urine were 96.30% and 66.67%(P＜0.01) but the combined positive (positive in serum and／or in urine) rate reached 100%, while in 109 chronic cases, they were 77.06% and 10.09%(P＜0.01) but the combined positive rate was not increased. In 100 healthy individuals from non-endemic areas, no positives were detected in serum and urine with false positive rate of 0%. The cross reaction rates in serum and urine of 51 cases with clonorchiasis were 9.43% and 0% but all were 0% in 48 cases with ancylostomiasis.

提要 应用McAb-Dot-ELISA平行检测血吸虫病人的血清和尿样(浓缩20倍)中日本血吸虫肠相关趋阴极循环抗原。81例急性血吸虫病患者的血清和尿样的阳性率分别为96.30%和66.67%，而血清和尿样组合检测的阳性检出率提高至100%。109例慢性血吸虫病患者的血清和尿样的阳性检出率分别为77.06%和10.09%，组合检测的阳性检出率没有提高。100份健康人的血清和尿样的假阳性率均为0%。54例华支睾吸虫病患者的血清和尿样的交叉反应率分别为9.43%和0%。48例钩虫病患者的血清和尿样的交叉反应率均为0%。

Serum and Ig in serum of the three sturgeons can react with serum of rabbit anti each of the three sturgeons' Ig, but cannot react with serum of rabbit anti Ig of carp Cyprynus carpio. The Ouchterlony results show that Ig of sturgeons are immune homologous in structure and sequence, but have little immune homologous characters compared with Ig of high-classification Osteichthyes such as carp.

免疫沉淀反应的结果显示，3种鲟鱼的血清及其Ig与相互之间的兔抗Ig血清有免疫沉淀反应，但与兔抗鲤Ig血清无免疫沉淀反应，这表明3种鲟科鱼类的Ig在结构和序列上是较为相似的，而与鲤鱼等高等硬骨鱼类的Ig存在较大的差别。

Results The reaction was not turbidness which was between modified reagent containing potassium hydroxide 143 mmol/L, tartrate kaliumna natrium 56.7 mmol/L and blood serum containing triglyceride 5.6 mmol/L,glucose polymer 60 g/L and need not sample blank.

结果含氢氧化钾 143mmol/L、酒石酸钾钠 56.7mmol/L的改良试剂与含 TG 5.6mmol/L、葡聚糖60g/L的血清反应均不显混浊，可不设标本空白。

Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDS PAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 and 18.5~12 ku, which reacted with 70%, 50%, 80% and 90% of asthmatic patient serum.

结果： 藜草花粉经电泳后得到12条蛋白质区带，分子质量依次为92， 63.1， 61， 52， 45， 43， 39.7， 38.9， 34， 31.6， 28.4和18.5~12 ku；免疫印迹可见4条sIgE反应带，蛋白分子质量依次为92， 34， 31.6和18.5~12 ku；与患者血清反应率分别是70%， 50%， 80%和90%；与血清sIgG结合的反应带有8条，蛋白分子质量为92， 61， 52， 45， 43， 39.7， 31.6和18.5~12 ku；与患者血清反应率分别是80%， 40%， 10%， 20%， 80%， 10%， 10%和100%。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

They couldn't be detected by the human sera against cysticercosis, and the reaction between GST-B? and the sera from pigs against oncosphere suggests that 62 be of immunogenicity.

免疫学分析(Western-blot)结果表明，二者均与囊虫患者的阳性血清不反应或反应很弱；B_2能与猪抗六钩蚴血清反应，说明B_2可能具有免疫活性。

Pestis proteins was fabricated and used to determine the humoral immune responses to Yersinia pestis in plague patients naturally infected spermophilus undulates, challenged and immunized rabbits.

通过与鼠疫患者血清、EV76疫苗株免疫兔血清、鼠疫菌感染兔血清及自然疫源地黄鼠血清反应，检测不同血清中抗体升高的种类及趋势。

Methods 34933 serum samples were screened by ELISA method on Treponema pallidum antibody. The samples with probable positive result were retested by toluidine red unheated serum test and Treponema pallidum particle assay. Results There were 559 serum samples with probable positive result.

对34933例标本采用酶联免疫吸附试验进行梅毒螺旋体的初筛，初筛结果为可疑阳性的样本进行梅毒螺旋体明胶凝集试验和甲苯胺红不加热血清反应素试验的检测。

A baby who is CMV-seronegative has not received antibodies to the disease from his mother while in utero.

CMV血清反应阴性的宝宝在妈妈的子宫里没有获得该病毒抗体。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。