血浆酶

The patient avoided eating food rich of protein for three days and fasting for 12 hours before the blood samples were obtained at 7:00-8:00 in the morning, and had laid on bed for 15 minutes at least, 2ml of the blood samples were taken from median antebrachial vein, put into the heparinized tubles, then centrifugating by 1500/minute for 10 minutes,obtained the plasma from the blood, the plasma sample were kept in -72℃ refrigerator, ELISA method was used to determine the homcysteine level.

患者在标本采集前3天避免食用高蛋白饮食，空腹12小时以上，清晨7：00－8：00采血，采血时平卧位，休息15分钟以上，采集肘正中静脉血2ml，置于肝素抗凝管，以1500转／分离心10分钟后，分离血浆，用酶联免疫吸附法测定各管的HCY含量。

Hemostatic mechanism total saponins of Yinfenglun were studied on plasma recalcified time, FT, KFTT and ELT. Result: TSY at different doses could markedly shorten bleeding time, reduce bleeding volume in mice. TSY also could shorten clotting time of mouse, rat and dog. TSY could influence both intrinsic coagulatian system and extrinsic coagulatian system, and had no effect of antifibrinolysis.

结果：荫风轮总苷不同剂量可明显缩短小鼠断尾出血时间，减少出血量，并能缩短小鼠、大鼠、犬凝血时间；可明显缩短家兔血浆复钙时间、凝血酶原时间和白陶土部分凝血活酶时间，但对大鼠优球蛋白溶解时间无明显影响，表明荫风轮总苷既能影响内源性凝血系统，又能影响外源性凝血系统。

Plasma levels of diamine oxidase and serum levels of interleukin-8(IL-8) were measured at different time points including before shock, 2h ,6h and 24h after successful resuscitation of shock. Ileum tissue at 5 cm away from approximal ileocecal portion was removed and the change of morphology , distribution of lymphocyte , count of PMN and villous height, width and surface area were examined and measured.

在休克前、复苏成功后2h、6h、24h 取外周血，测定血浆二胺氧化酶含量及血清白介素-8(IL-8)水平；取距回盲部近端5cm 处回肠组织进行光镜组织形态学观察，淋巴细胞分布检测和多形核中性粒细胞计数，并测量回肠绒毛高度、宽度和表面积。

This enzyme catalyses the conversion of angiotensin I to angiotensin II in the blood plasma and endothelia and prevents the breakdown of bradykinin.

这种酶催化的血管紧张素一血管紧张素血液中的血浆和内皮二，防止故障的激肽转换。

Objective To study the linkage of cholesteryl ester transfer protein and paraoxonase (PON1) loci with development of gallstone and plasma lipids or apolipoprotein levels.

目的探讨胆固醇酯转移蛋白基因和二乙基对硝基磷脂酶(PON1)基因位点与胆囊结石发生及血浆脂质水平的连锁关系。

Plasma Ang Ⅰ and Ang Ⅱ levels, chymase mRNA levels, arteries' PCNA positive percents and the thickness of carotid artery were measured.

通过测定血浆Ang Ⅰ及AngⅡ住水平、糜酶 mRNA表达水平、颈动脉细胞增殖核杭原阳性率及颈动脉各层厚度观察曲尼司特对犬颈动脉损伤后狭窄的作用。

Methods; In APS treatment group and control group, levels of insulin, C-peptide, myocardial enzymes, glycosylated serum protein and angiotensin were measured; Expression levels of chymase and angiotensin-coverting enzyme mRNA were detected by RT-PCR.

检测APS治疗组和对照组DM仓鼠的胰岛素、C肽、心肌酶谱、糖化血清蛋白和血浆。

Results As compared to DM control group, levels of GSP, myocardial enzymes and myocardial Ang Ⅱ were much lower in APS group, while levels of insulin, C-peptide and plasma Ang Ⅱ were the same. Levels of expression of collagen Ⅰ and the ratio of collagen Ⅰ/collagen Ⅲ in APS group were lower than those in DM group. APS group showed lower levels of chymase mRNA expression and activity than those in DM control group, while there was no diference in ACE mRNA expression and activity between the two groups.

结果 APS组血糖、糖化血清蛋白、心肌酶谱和心肌Ang Ⅱ水平较DM组显著下降，胰岛素、C肽、血浆AngⅡ水平和DM组无差异；APS组心肌Ⅰ型胶原表达和Ⅰ/Ⅲ型胶原比值较DM组显著下降；APS组chymase mRNA表达和活性均显著低于DM组，ACE基因的表达和活性与DM组无显著性差异。

While levels of insulin. C-peptide and plasma Ang Ⅱ were similar. APS group showed lower levels of chymase mRNA expression and activity than those of DM control group, while there was no diference in ACE mRNA expression and activity between two groups. Compared with DM group, expression level of p-ERK1/2 in APS group was much lower.

结果：APS治疗组GSP、心肌酶谱和心肌AngⅡ水平较对照组显著下降；血胰岛素、C肽、血浆AngⅡ水平与对照组无显著差异；APS组chymase mRNA表达和活性均显著低于对照组，ACE基因的表达和活性与对照组无显著差异；APS组心肌p-ERK1/2含量较对照组明显降低。

Methods In APS group and diabetes mellitus control group, levels of serum glucose, insulin, C peptide, myocardial enzymes, glycosylated serum protein and angiotensin Ⅱ in plasma and myocardial were tested; Immuno-histochemistry was used to measure expression levels of type Ⅰ and type Ⅲ collagen; Expression levels of chymase Mrna and angiotensin-converting enzyme mRNA were detected by using fluorescent quantitative polymerize-chain-reaction; Activities of chymase and ACE were tested by radioimmunoassay.

检测APS治疗组和DM对照组仓鼠的血糖、胰岛素、C肽、心肌酶谱、糖化血清蛋白水平，血浆和心肌组织的AngⅡ含量；免疫组化法检测心肌Ⅰ、Ⅲ型胶原；荧光定量PCR法检测心肌chymase和ACE mRNA表达；放免法检测chymase和ACE活性。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。