血小板减少

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01)；若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%，特异性为94.3%；4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05)，且大于各单个抗体检测敏感性。

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P .01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48, and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%.

结果 特发性血小板减少性紫癜组4种单抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异（P＜0.01）；若将ITP组患者4种单抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.17%，特异性为94.3%；4种单抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验（P＜0.05，且大于各单个抗体检测敏感性。

To observe the morphological characteristics and hematopoietic function of bone marrow megakaryocyte in children patients with idiopathic thrombocytopenic purpura,and to preliminary analyse the cause and mechanism of thrombocytopenia.

本研究观察特发性血小板减少性紫癫患儿骨髓巨核细胞形态与造血功能的改变，初步分析其血小板减少的发生机制。

Objective: To explore the relationship between thrombopoietin levels and blood platelet count, megakaryocyte count in patients with thrombocytopenia and provide theoretical basis of clinical application of rhTPO.Methods: We measured blood platelet count, megakaryocyte count and the serum level of TPO in three group patients of differential diagnosis of thrombocytopenia .

目的 通过对不同类型的血小板减少患者进行血小板计数、巨核细胞计数，血小板生成素的测定，来探讨三者之间的关系及各种疾病造成血小板减少的原因，针对不同病因提出不同的治疗方案，为重组人血小板生成素的临床应用提供理论依据。

It is important to differentiate immune thrombocytopenia from nonimmune thrombocytopenia because of the different clinical treatment, but untill now there is no satisfactory laboratory tests could be used.

非免疫性血小板减少性紫癜，是指由非免疫因素引起的血小板减少，如化疗后骨髓抑制、急性白血病、脾功能亢进以及恶性肿瘤骨髓转移等引起的血小板减少。

The diagnosis of ITP is made clinically by exclusion of other causes of thrombocytopenia, the absence of splenomegaly, and the finding of normal or increased megakaryopoiesis on bone marrow examination. The nonimmune thrombocytopenia is caused by some nonimmune factors, such as acute leukemia, hypersplenism and post-chemotherapic bone marrow suppression.

特发性血小板减少性紫癜的诊断仍只是一种排除性的临床诊断，其国内诊断标准为多次化验检查血小板计数减少，脾脏不增大或仅轻度增大，骨髓检查巨核细胞数增多或正常，有成熟障碍，并且具备以下五点中的任何一点：泼尼松治疗有效，切脾治疗有效，血小板相关抗体增多，血小板相关补体增多，血小板寿命测定缩短，同时应排除继发性血小板减少症。

Prophylactic infusion thrombocyte can not prevent cerebral hemorrhage completely.To preclud the correlated cause may be important measure to decrease the death rate of cerebral hemorrhage with thrombocytopenia.

血小板减少性脑出血预后差，预防性血小板输注不能完全阻止脑出血的发生，控制和消除诱发脑出血的其他相关原因可能是减少血小板减少性脑出血的重要措施。

Prestorage and poststorage leukocyte filtration apheresis platelets were transfused respectively to patients with malignancy accompanied by serious thrombocytopenia after chemotherapy. Peripheral blood platelet counts were made at 1h, 24h after platelet transfusion. The effectiveness was assayed by correct count increment. The incidence rates of FNHTR were explored.

46例肿瘤化疗后血小板减少患者，分别输注保存前过滤单采血小板与保存后过滤单采血小板，输注后1h及24h后检测外周血小板计数，以校正血小板计数增殖判定输注效果，并考查FNHTR发生率。

It is concluded that the positive rate of platelet antibody screening is very high in patients with hematologic malignancies, the coincidence rate of platelet antibody crossmatch in 249 blood samples is between 40% and 48%, and the effeciency of using crossmatched platelets in clinic is enhanced significantly.

微柱凝胶免疫分析技术在血小板抗体筛选中的应用大部分免疫性血小板减少性紫癜患者血小板膜上可测出血小板相关抗体，包括IgG、PA IgM、PA IgA，其中一项出现阳性，即为血小板抗体检测阳性，而且其量往往与外周血的血小板数量呈负相关，因此检测这种血小板相关抗体有助于免疫性血小板减少性紫癜的诊断［1］。

The identification of thrombopoietin, and the demonstration that administration to normal experimental animals and pre-and post-chemotherapy patients with advanced cancer with recombinant TPO results in a several-fold increases in platelet count, has opened the possibility of using TPO to rescue patients from lifethreatening thrombocytopenia with above mentioned situations, while the cloning of TPO cDNA also has made it possible to treat thrombocytopenia using TPO gene.

各种原因所致的血小板减少症，常因血小板严重减低而出现致命性出血合并症。而目前唯一有效的治疗措施是输注血小板。但由于目前血小板输注这一治疗手段尚存在许多弊端，人们迫切需要早日解决这一难题。血小板生成素(thrombopoietin， TPO)基因的克隆为采用基因治疗手段治疗血小板减少症带来了希望。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐