血小板

Fluorescein isothiocyanate labeled polyclonal goat antihuman immunoglobulin antibody was added, and flow cytometer was used to detect bead-platelet-associated autoantibodies-antihuman immunoglobulin antibody complex. Results The fluorescene ratio of four monoclonal antibodies was significantly different (P.01) between the idiopathic thrombocytopenic purpura patients and either the non-ITP patients or the normal controls. If the upper limit of normal control was set as cutoff value, ratios of greater than 1.37, 1.24, 1.48 and 1.19 were considered positive for the four monoclonal antibodies respectively. The flow cytometric bead assay had an overall sensitivity of 73.17% and a specificity of 94.29%. The overall sensitivity was significantly higher (P.05) than that of modified indirect MAIPA and that of using single antibody.

结果 特发性血小板减少性紫痕组4种羊抗荧光强度比值与非ITP血小板减少组和正常对照组有显著性差异(P.01)；若将ITP组患者4种羊抗荧光强度比值分别大于正常对照组上限1.37、1.24、1.48和1.19判断为阳性，则流式微球技术检测血小板特异性自身抗体的敏感性为73.2%，特异性为94.3%；4种羊抗联合检测总体敏感性明显高于改良间接单抗特异的血小板抗原固定试验(P.05)，且大于各单个抗体检测敏感性。

Objective: To explore the relationship between thrombopoietin levels and blood platelet count, megakaryocyte count in patients with thrombocytopenia and provide theoretical basis of clinical application of rhTPO.Methods: We measured blood platelet count, megakaryocyte count and the serum level of TPO in three group patients of differential diagnosis of thrombocytopenia .

目的 通过对不同类型的血小板减少患者进行血小板计数、巨核细胞计数，血小板生成素的测定，来探讨三者之间的关系及各种疾病造成血小板减少的原因，针对不同病因提出不同的治疗方案，为重组人血小板生成素的临床应用提供理论依据。

Four parameters of platelet were detected (using Japan East Asia Sysmex F-800 hematocyte automatic counting machine) and compared.

采用日本东亚SysmexF-800型血细胞自动计数仪检测了150例肝炎后肝硬化、150例重型肝炎、114例急性黄疸型肝炎、107例慢性活动型肝炎和50例健康成人的血小板功能4项指标：血小板计数、平均血小板体积、血小板压积和血小板分布宽度。

Mechanistically, expression of a molecule known as P-selectin by the platelets was crucial for mediating the platelet-neutrophil interaction, which induced platelets to produce proinflammatory factors such as TXA2. The authors therefore suggest that disrupting the platelet-neutrophil interaction or blocking the proinflammatory factors produced as a result of this interaction might provide new therapeutic targets for the treatment of individuals with ALI.

具体来说，体现在分子水平上就是由血小板产生的血小板蛋白对于调解血小板和中性粒细胞交互影响起着关键作用，它能够诱导血小板产生致炎因素如血栓素A2等，该文作者因此认为，或许破坏血小板与中性粒细胞间的相互作用或者阻断相互作用所产生的致炎因素可以提供一种新的方法用于治疗急性肺损伤。

Prestorage and poststorage leukocyte filtration apheresis platelets were transfused respectively to patients with malignancy accompanied by serious thrombocytopenia after chemotherapy. Peripheral blood platelet counts were made at 1h, 24h after platelet transfusion. The effectiveness was assayed by correct count increment. The incidence rates of FNHTR were explored.

46例肿瘤化疗后血小板减少患者，分别输注保存前过滤单采血小板与保存后过滤单采血小板，输注后1h及24h后检测外周血小板计数，以校正血小板计数增殖判定输注效果，并考查FNHTR发生率。

Hemostasis is the result of interdependent and complex molecular and cellular pathways that govern vascular integrity and rheology. The interaction between the systemic and local pathways provides the necessary actions required to regulate vascular bed-specific hemostatic activity. Alterations in the dynamic balance can result in marked vascular pathophysiology such as bleeding diatheses or hypercoagulability.

本次研究针对的是先心病患者血小板的活化状态，血小板的活化状态常用来表示在各种实验和临床条件下血小板的性质和功能变化特征，我们研究先心病患儿血小板的活化状态是为了证明一种假设，即血小板的活化可能在这些患儿的止血功能异常中起作用。

Methods The lyophilized platelets were rehydrated after being preserved 30 days. The change of morphology and ultrastructure of platelets was observed under the light microscope and transmission electron microscope. The survival rate and apoptosis rate of platelets were analyzed by flow cytometry. The aggregation rates of rehydrated lyophilized platelets were measured by using 1 U/mL thrombin.

以新鲜血小板为对照，以保存30d后再水化的冻干血小板为实验对象，分别在倒置相差显微镜和透射电镜下观察血小板的细胞形态和超微结构，用流式细胞仪检测血小板凋亡率，并以1×10^3U/L凝血酶为诱导剂检测再水化血小板的聚集活性。

The primary cause of the acquired platelet defect is thought to be activation and release of alpha granules during extracorporeal circulation. Before CPB PRP was prepared by obtaining the required amount of patient's whole blood by autologous plateletpheresis. PRP could be reinfused after operation in order to save the function and quantities of the platelets. On the other hand PRP could be made into autologous platelet gel. APG contains supraphysiologic amounts of growth factors and has adequate tensile strength and adhesive ability.

CPB前，利用自体血小板分离技术可将部分血小板从患者全血中分离出来制成富血小板血浆（Platelet-rich plasma PRP），PRP可在术后回输，以达到血小板数量和功能的双重保护，另一方面可将PRP制备成自体血小板胶，APG中含有丰富的生长因子，并且具有足够的抗张强度和粘性，因此可起到术中止血，封闭伤口，促进胸骨及伤口愈合的作用。

The primary cause of acquired platelet defect is thought to be activation and release of alpha granules during CPB. Before CPB, platelet-rich plasma was prepared by obtaining the required amount of patient's whole blood by autologous plateletpheresis. PRP could be reinfused after operation in order to protect the function and quantities of the platelets. On the other hand, PRP could be made into autologous platelet gel. APG contains supraphysiologic amounts of growth factors, and has adequate tensile strength and adhesive ability. Therefore, it can be used for hemostasis in operation, sealing wound and enhancing incision or dehiscent sternal wounds healing.

CPB前，利用自体血小板分离技术可将部分血小板从患者全血中分离出来制成富血小板血浆(platelet-richplasma，PRP)，PRP可在术后回输，以达到血小板数量和功能的双重保护，另一方面可将PRP制备成自体血小板浆(autologousplateletgel，APG)， APG中含有丰富的生长因子，并且具有足够的抗张强度和粘性，因此可起到术中止血、封闭伤口、促进胸骨及伤口愈合的作用。

Liu. The factors affect the quality of platelets during the course of preparation, two cryobiology parameters of platelets and the new characteristics of new subpopulations in cryopreservated platelets were studied based on methods selected or set up in our laboratory.

在遴选和建立血小板新质量检测新方法的基础上研究冰冻血小板的制作过程中血小板质量影响因素和冰冻血小板的部分低温生物学参数以及冰冻血小板的新生物学特性。

John Robbins lives with his family in Santa Cruz, California.

约翰罗宾和家人居住在加利福尼亚州的圣克鲁斯县。

The international background is mainly the economic ties among different nations tented to be widen and deepen at the peace and development times.

国际背景主要是和平与发展时代条件下的世界各国经济贸易联系的日益加深。