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血小板

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Methods Whole blood sample (2ml) acquired from 12 volunteers via a large antecubital vein was heparinized with 10U/ml heparin sodium and ice-bathed down to 4℃ and divided into the following groups: control group , PAF group , FMLP group, PAF+FMLP group , FMLP+lidocaine (10-4 M) group and lidocaine (10-4M,10-5M,10-6M )groups . the expression of CD11b in neutrophils was detected by flow cytometry in different groups .

选12名健康的成年志愿者,每位志愿者抽肘前静脉血2ml,肝素抗凝,置冰浴中降温至4℃分组如下:对照组,血小板活化因子组,趋化寡肽组,血小板活化因子+趋化寡肽, FMLP+利多卡因(10-4 M)组和P+F+利多卡因(10-4M,10-5M和10-6M)组,分别测定PMN CD11b的表达水平。

BACKGROUND AND PURPOSE: Antiplatelet medication use and reduced platelet activity may be associated with mortality after intracerebral hemorrhage.

背景和目的:抗血小板药物的使用以及血小板活性降低可能与脑出血后死亡率相关。

54 cases' RA activity group compared with 20 cases' relief group and 25 cases' control group, then analyzed the index of patient's condition of RA patients' platelet parameter (ESR、CRP、IgG、IgA、IgM、C3、C4、RF), and associativity of clinical symptom such as arthralgia, arthrocele, subdermal induration, violet and dark oral lip, scaly skin, ecchymosis of corpus linguuae, frequency of petechia.

比较RA活动组54例,缓解组20例和正常对照组25例的血小板参数变化,分析54例活动期RA患者血小板参数与病情活动指标(ESR、CRP、IgG、IgA、IgM、C3、C4、RF)及临床症状(关节疼痛数、关节肿胀数、皮下硬结、口唇紫黯、肌肤甲错、舌体瘀斑或瘀点出现率)的相关性。

"Anti-platelet drugs are often used to reduce intima thickening, for example, restenosis...after angioplasty. Perhaps a different method of inhibiting platelet activation would be to drink dealcoholized beer," Dr. Bassus says."Conversely, the acute consumption of alcohol beverages causes an increase of thrombin generation, suggesting a thrombotic risk in case of acute alcoholic intoxication."

血小板药物一般被用来降低血管内膜的肥厚症状,如该药物可预防血管修复手术后的血管狭窄现象,Bassus表示,也许抑制血小板活化的另一种方法就是投予去醇啤酒,然而,过度的酗酒反而会造成凝血酵素的增生,也就是说,急性酒精中毒通常会有缺血的风险。

Under the direct current electric field, the expression of PDGFR were increased obviously and distributed asymmetricly on the membrane of VSMC. This receptor were mainly focused on the cathodal side of cellular membrane in electric field. The AT1R were also enhanced in the cells after exposure to the electric field, and the expression of AT2R had showed the changeless.

改进的电场干预装置作用下,血管平滑肌细胞膜血小板源生长因子受体表达增加,部分细胞内血小板源生长因子受体表达上调,呈不对称分布,在电场负极面较集中;细胞中血管紧张素Ⅱ1型受体表达亦增加,但无明显不对称分布现象;血管紧张素Ⅱ2型受体表达改变不显著。

In our study, we also observed the different influence between TTB-USA and RFCA on blood coagulable states (by examining the change ofα-granule membrane protein、Tissue type plasminogen activatior、Plasmingen activator inhibitor-1)、 endothelium function (by examining the change of endothelin、nitric oxide) and myocardial injury (by examining the change of creatine kinase、MB isoenzy of creatine kinase and cardiac troponin T).

为观察超声消融与射频消融对血凝状态、内皮功能及心肌损伤的影响,进一步对超声消融的安全性进行评价,我们在插管前、插管后、消融后即刻、消融后30min及48小时5次抽静脉血,分别测定血小板α-颗粒糖蛋白(GMP-140)阳性表达活化血小板百分率、内皮素、一氧化氮、组织型纤溶酶原激活剂及纤溶酶原激活剂抑制物-1(PAI-1)的变化;于消融前、消融后即刻、2hr、8hr、24hr、48hr、72hr抽取静脉血,检测肌酸激酶、肌酸激酶MB同工酶及心肌肌钙蛋白T的变化。

The platelet count, aggregation functions, adhesive ability, hypotonic shock reaction and CD62p expression of platelets preserved at normal temperature, cryopreserved on same day of collection and after storage for 3 days were detected, and possibility of using platelets cryopreserved after storage for 3 days in clinic was evaluated by comparison of contractable clinical cases.

对当天冷冻和保存3天后再冷冻血小板进行了计数,并检测聚集力、黏附力以及CD62p的表达,并通过可对比性病例观察保存3天后再冷冻与当天冷冻血小板临床应用的可能性。

PDGF-BB is the most effective subtype in the PDGF family on the metabolism of osseous tissue or the treatment of wound healing, and its clinical application was approved firstly among the growth factors although it was used to treat the decubital ulcer of diabetes.

本研究通过分子克隆方法获取人血小板衍生生长因子B链成熟肽基因,构建原核表达载体;利用大肠杆菌体系原核表达人血小板衍生生长因子B链前体蛋白,并纯化、复性进而获得具有功能活性的PDGF-BB;并对获得的重组rhPDGF-BB蛋白进行体外功能活性鉴定,同时,初步探讨PDGF-BB在毕赤酵母中的表达,为寻找更优化的表达纯化方法提供实验学依据。

Objective:To study Effects of dehydrocorydaline on cAMP and cGMP concentration in rabbit platelets in vitro.

目的:研究脱氢紫堇碱对兔血小板中cAMP 和cGMP含量的影响以探讨其抑制血小板聚集的作用机制。

Results A large quantity of floccose blocks were observed in the blood samples in which platelets were removed by natural sedimentation.

方法应用自然沉降和离心方法去除血液标本中的血小板,用凝聚胺方法进行血小板去除前后抗体筛检和交叉配血试验。

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