血小板

When the results show platelet aggregating mega platelet platelet barograph shifting right or the tail of curve lifting,in order to avoid false decreasing of platelet count caused by sample laid in short time and anticoagulant mixed incompletely, platelet count shooed be tested with hand-method.If necessary platelet shape should be observed under the oil immersion lens.

末梢血计数血小板要在60秒钟以后测方可以获得较准确的参考数据，同时要结合血小板直方图，当提示有血小板聚集，大血小板，直方图右移尾部上翘者，要进行油镜下观察血小板形态，同时进行手工法计数血小板。

The aim of this study was to explore the potential relationship between the enhancement of instant hemostatic function in vivo of cryopreserved platelets and its procoagulative related molecule activities. The ability of platelet binding factor Ⅴ, density of GPIb-Ⅸ-Ⅴ(CD42a) at platelet member surface were detected by flow cytometry, the clotting time induced by activated platelets were evaluated by coagulometer and platelet count, MPⅤ and PDW were measured by hemocytometer before and after fresh platelets were cryopreserved.

为了探索血小板冰冻保存后膜表面促凝血活性相关分子变化与冰冻血小板体内即刻止凝血功能增强之间的可能联系，用流式细胞术检测了新鲜血小板冰冻保存前后Ⅴ因子结合能力、血小板膜表面GPIb-Ⅸ-Ⅴ分子(CD42a)密度，用血凝仪测定激活血小板诱导血浆凝固时间变化，用血细胞计数仪测定血小板计数、MPⅤ和PDW。

The platelet count,platelet aggregation rate,lactic acid content and CD62p expression level of platelet were detected on 0,1,2,3,4,5 days of platelet preservation.

分别在血小板保存0、1、2、3、4、5天检测血小板量数量、血小板聚集反应功能、血小板液乳酸含量和血小板CD62p表达量。

Objective To explore the effect of-epigallocatechin gallate, main component of green tea, on platelet aggregation and activation, to establish the foundation of development of new anti-platelet drug.

目的 对绿茶的主要成分表没食子儿茶素没食子酸酯对血小板聚集、人富含血小板血浆产生TXB2、P-selectin以及血小板膜糖蛋白P-selectin表达水平的影响进行研究，探讨EGCG对血小板聚集、活化的影响，为进一步开发抗血小板药物提供依据。

The diagnosis of ITP is made clinically by exclusion of other causes of thrombocytopenia, the absence of splenomegaly, and the finding of normal or increased megakaryopoiesis on bone marrow examination. The nonimmune thrombocytopenia is caused by some nonimmune factors, such as acute leukemia, hypersplenism and post-chemotherapic bone marrow suppression.

特发性血小板减少性紫癜的诊断仍只是一种排除性的临床诊断，其国内诊断标准为多次化验检查血小板计数减少，脾脏不增大或仅轻度增大，骨髓检查巨核细胞数增多或正常，有成熟障碍，并且具备以下五点中的任何一点：泼尼松治疗有效，切脾治疗有效，血小板相关抗体增多，血小板相关补体增多，血小板寿命测定缩短，同时应排除继发性血小板减少症。

The loading process of trehalose has no effect on apoptosis of platelets. The efficacy of trehalose plus phosphate in suppressing apoptosis of platelets during cryopreservation is superior to that of only trehalose. Compared to 6% DMSO and 2% DMSO plus TS, trehalose plus phosphate can retain the same platelet count, superior hypotonic shock response and platelet aggregatory reaction to ADP, and lower apoptosis rate of platelets. Platelets cryopreserved with trehalose plus phosphate can retain intact ultrastructure.

结论血小板负载海藻糖的过程不会促进血小板的凋亡；海藻糖保存血小板时，联合应用磷酸盐在抑制血小板凋亡方面的效果优于单独应用海藻糖；海藻糖联合磷酸盐冻存血小板的长期效果较稳定，其维持血小板回收率的效果与6%DMSO及2% DMSO联合TS相同，维持血小板低渗休克反应、聚集功能及抑制血小板凋亡的效果优于6%DMSO及2%DMSO联合TS，并能较好地维持血小板的超微结构。

Expression rate induced by thrombin was 50.88% and PAC?1 re?expression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activation?inhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activation?inhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freeze?drying procedure.

添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

CD62p reexpression rate induced by thrombin was 50.88% and PAC1 reexpression was 54.55%. It is concluded that the ability of recovered lyophilized platelets added with reversible activationinhibitors, DMSO and trehalose to aggregate and coagulate has showed no significant difference as compared with FPRP. The reversible activationinhibitors can decrease CD62p expression of lyophilized platelets, and may enhance their survival ability and prolongate survival time. Therefore the efficiency of lyophilizing platelets can be improved based on this freezedrying procedure.

添加血小板可逆性激活抑制剂，海藻糖和DMSO后的冻干血小板，其聚集活性和促凝血功能与新鲜血小板无明显差异，血小板可逆性激活抑制剂降低了冻干血小板的CD62p表达，增强了冻干血小板的生存能力，因而延长了其生存时间，因此可以该冻干方法为基础进一步提高冻干保存血小板的效率。

It is concluded that the positive rate of platelet antibody screening is very high in patients with hematologic malignancies, the coincidence rate of platelet antibody crossmatch in 249 blood samples is between 40% and 48%, and the effeciency of using crossmatched platelets in clinic is enhanced significantly.

微柱凝胶免疫分析技术在血小板抗体筛选中的应用大部分免疫性血小板减少性紫癜患者血小板膜上可测出血小板相关抗体，包括IgG、PA IgM、PA IgA，其中一项出现阳性，即为血小板抗体检测阳性，而且其量往往与外周血的血小板数量呈负相关，因此检测这种血小板相关抗体有助于免疫性血小板减少性紫癜的诊断［1］。

The identification of thrombopoietin, and the demonstration that administration to normal experimental animals and pre-and post-chemotherapy patients with advanced cancer with recombinant TPO results in a several-fold increases in platelet count, has opened the possibility of using TPO to rescue patients from lifethreatening thrombocytopenia with above mentioned situations, while the cloning of TPO cDNA also has made it possible to treat thrombocytopenia using TPO gene.

各种原因所致的血小板减少症，常因血小板严重减低而出现致命性出血合并症。而目前唯一有效的治疗措施是输注血小板。但由于目前血小板输注这一治疗手段尚存在许多弊端，人们迫切需要早日解决这一难题。血小板生成素(thrombopoietin， TPO)基因的克隆为采用基因治疗手段治疗血小板减少症带来了希望。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。