血培养

Methods The peripheral blood lymphocytes,sera of 55 patients with rheumatic carditis and a specific antigen from group A streptococcal cell membrane were prepared and used as stimulants to test the heart tissue cell procoagulant activity in culture.

用风湿性心脏炎患者的外周血淋巴细胞、血清及A组乙型溶血链球菌膜抗原作为刺激物，测定体外培养的人胚心肌细胞促凝血活性。

In the present experiment, the tender stems of Dracaena cambodiana were used as explants to establish a two-step tissue culture and regeneration system. The effects of types and concentrations of plant hormones on the induction of callus were studied. Based on these, the reactions of callus regeneration to different mutation-induced approaches (60Co irradiation, EMS and colchicines), the regeneration ability of the treated callus as well as the characters diversities (including the appearance traits and cytological observations) of the regeneration plants were investigated.

本试验以龙血树幼嫩茎段为材料，通过对龙血树幼嫩茎段两步培养法再生的研究，建立了龙血树幼嫩茎段离体再生体系，证明了愈伤组织的发生和激素种类与浓度的关系；并在此基础上研究了愈伤组织再生对不同人工诱变方法（60Co辐射、EMS、秋水仙素）的反应、被处理愈伤组织再生能力和再生植株性状多样性、以及再生植株不同生理角度的变异性，得到了2株变异株系。

The apical buds and axillary buds of Dracaena cambodiana Pierre ex Gagnep. were inoculated on MS medium containing 1 mg/L BA, 0.1 mg/L NAA, 100 mg/L PVP and 30 g/L sucrose for inducing shoots. After cultured for 40~50 d, the shoots bourgeoned. They were then transferred onto MS medium supplemented with 2 mg/L BA, 0.5 mg/L KT and 30 g/L sucrose for inducing clustered buds. After 25~10 d, the clustered buds formed. The proliferation rate of the clustered buds was 300%~500% per month.

以海南龙血树的顶芽和侧芽作为外植体，把其接种于MS+BA 1 mg/L+NAA0.1 mg/L+PVP100 mg/L+蔗糖30 g/L培养基上培养40~50 d可诱导其腋芽萌发，再把萌发后所形成的新芽切割下来接种于MS+BA 2mg/L+KT 0.5 mg/L+蔗糖30 g/L的培养基上培养25~30 d可诱导形成丛生芽，丛生芽在继代培养过程中每25~30 d可增殖3~5倍。

The object of this study is to cancerous and normal cells intimacy of hematoporphyrin derivative for diagnosis and therapy cancer, fluorescence spectra of cancerous and normal cells are measured using a ultrashort pulses laser spectral technique and picosecond time-correlated single-photon counting system.

研究用于癌症诊断与治疗的光敏剂血卟啉(hematoporphyrin derivative， HPD)对人体癌细胞与正常细胞的亲和性，采用超短脉冲激光光谱技术和皮秒时间相关单光子计数系统，测量经血卟啉培养的活体癌细胞与正常细胞的荧光光谱，观测到癌细胞样品在创645nm处具有特有的光谱谱峰；测量不同浓度血卟啉荧光光谱，观测到随着血卟啉浓度的逐渐增大，在645nm处出现的光谱峰也随之显著增强。

The cells of 2~ passage were used for proliferation kinetics studying by MTT colorimetric method.The pasagcd chondrocytes' phenotypes were as- sessed by safranine O staining and immunostaining for typeⅠ,Ⅱcollagens and aggrecan.Results For in vitro culturing of AHAC,human AB serum was superior to FBS,and the optimal concentration of human serum was 10%.But for FBS,20% was better,A similar result was got with or without the growth factors contained.

结果对于AHAC，人AB血清培养优于FBS；合适的人AB血为10%；合适的FBS浓度为20%；无血清培养细胞增殖非常缓慢；不加因子用人AB血清培养细胞梭形化明显，加用生长因子后与使用FBS在形态上一致。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

Vibrio parahaemolyticus 1211U was cultured in sterilized sea water by the process of filtration at 4 ℃;50-60 days later, plate count and most probable number showed that there were no culturable bacteria.

将副溶血弧菌菌株1211U培养于低温贫养条件下，以平板菌落计数法和最大近似数法检测可培养细菌数，50～60d后表明可培养数下降为零。

Methods Human umbilical cord blood mononuclear cells were isolated by Ficoll density-gradient centrifugation. EGM-2 culture fluid was added, and then cells were plated on dishes coated with human fibronectin. After 48 h, the nonadherent cells were collected and replated on fibronectin-coated dishes.

采用Ficoll密度梯度离心法从人脐血中分离单个核细胞，加入EGM-2培养液，接种于包被人纤连蛋白的培养皿中贴壁培养，收集48h后的悬浮细胞重新贴壁培养至第7天，利用免疫荧光和流式细胞术鉴定。

Depositing cells were incubated in high-sugar DMEM culture medium containing 15% fetal bovine serum, 100 U/mL penicillin, and 100 U/mL Streptomycin, then cells were seeded in a 25 T culture bottle after cell density was adjusted to 1×109 L-1, and incubated at 37 ℃, 0.05 volume fraction CO2 with saturated humidity. After 72 hours, the culture medium was replaced, and red blood cells and other nonadherent cells were removed. Cells began to passage at 70%-80% confluence.

实验方法：穿刺抽取15 mL脐带血，密度梯度法分离吸取分层界面的白色环形絮状物，其中富含单个核细胞，离心后沉积细胞用含15％胎牛血清、100 U/mL青霉素、100 U/mL链霉素的DMEM高糖培养基悬浮，调整细胞密度为1×109 L-1接种于25 T培养瓶内，放置在37 ℃、体积分数为0.05的CO2饱和湿度培养箱中培养，72 h后更换培养基，去除红细胞及其他未贴壁细胞，至70％~80％融合时传代。

The most pathogenic isolate, VIB 645, contained two closely related hemolysin genes, which were cloned and sequenced. In this study, vhhA was cloned into an expression plasmid pET-24d.

本研究将哈维氏弧菌的溶血素基因vhhA克隆到大肠杆菌的表达载体pET-24d，VHH溶血素作为一种带六个组氨酸的融合蛋白在大肠杆菌表达菌株BL21(DE3)中得到了过量表达，重组大肠杆菌及其培养上清液在鱼血平板上有很强的溶血活性。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。