血培养

Through the analysis of the contents of loureirins of the zymolysis products of fungus with different culture time, it can conclude that dragon's blood is the products of interactions between Dracaena cochinchinenis and the animalcula.

对比部分微生物不同培养时间的发酵产物，可以得出龙血竭的形成是剑叶龙血树和它损伤后感染的微生物相互作用的结果，并且从微生物回接实验的结果来看，某些微生物对剑叶龙血树龙血竭的形成有一定的促进作用。

objective comparison of 120 example cervical wecretion directly smear and culture.methods one sterile swab directly smear;gram stain;bacterioscopy, others swabs inoculate chocolate plate;blood plate;shaborose plate;culturing 24 hours then identification.results ng and bv directly smear isolates rate higher than culture;butfung culture isolate rate higher than direct smear to bacterioscopy.conclusion cervical wecretion directly smear to bacterioscapy has rapidly and simply diagnostic value

摘 要］目的：比较120例妇科宫颈分泌物直接涂片镜检与培养结果的分析。方法：宫颈分泌物直接涂片、革兰染色、显微镜检与宫颈分泌物接种于巧克力板、血平板、沙氏板进行培养和鉴定。结果：对分泌物中的致病菌而言，g-双球菌和阴道加德纳菌的直接涂片镜检阳性率较培养高；霉菌的培养阳性率较直接涂片镜检率高。结论：妇科宫颈分泌物直接涂片镜检具有快速、直观、简便的诊断价值。

Methods: Influenza viruses were isolated with the means of cultured in MDCK cell and embryonated eggs. The cross hemagglutination inhibition test was used for antigenic analysis and determined nucleotide sequence of HA1 genes of the influenza A (H3N3) virus.

采用MDCK细胞培养和鸡胚培养法分离流感病毒，交叉血凝抑制法进行抗原分析，对甲3型流感病毒的HA1血凝素基因作了核苷酸序列测定。

Majority of aderent cells from BM were fibroblast-like cells, and small parts were endothelioid cells. Aderent cells from PB and CB at the fifth generation contained more endothelioid cells and mononuclear and macrophage-like cells besides fibroblast-like cells.

初始培养和传代培养后，骨髓间充质干细胞多为成纤维样细胞，仅有少量内皮样细胞；而传至第5代的外周血、脐带血间充质干细胞除有成纤维样细胞外，还有较多的内皮样细胞和单核－巨噬样细胞。

LPS stimulation can advance the maturation of DCs, which showed more typical morphology, expressed higher cell special phenotyes, has more powerful activation of proliferation of homogenic lymphocyte and secreted higher levels of cytokine. The character had no obvious dose dependence on LPS. It indicated that LPS can

说明在DC诱导培养的后期，LPS可以进一步促进DC的成熟，由此可以进一步推断DC疫苗经体外诱导培养后注入伴有肠源性内毒素血症的肝病患者体内其疗效将会更加明显，这为DC疫苗用于内毒素血症患者的免疫治疗提供了实验依据。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

Cytokine-producing CD4＋ T cells were quantified by 3-color flow cytometry after bring stimulated with phorbol myristate acetate and ionomyccin. After being stimulated with PHA, the levels of IFN-γ and IL-4 in culture supernatants were measured by ELISA.

同时分离外周血单核细胞，经植物血凝素刺激培养后，应用酶联免疫吸附方法检测培养上清液中IFN-γ和IL-4水平。

To compare the growth characteristics of non-hematopoietic adult stem cells derived from rat fetal blood and rat bone marrow in vitro, and to study the differentiation of these stem cells into neuron-like cells in vitro,the fetal blood of pregnant rats and bone marrow of adult rats were sterilely collected; mononuclear cells were isolated by using standard Ficoll-hypague techniques and then cultured in DMEM/LG containing 10% fetal bovine serum.

为了比较大鼠胎血和骨髓中非造血成体干细胞（non-hematopoietic adult stem cells，NASC）体外培养过程中的生长特性及体外诱导两者向神经元样细胞分化的异同，在无菌条件下采集孕大鼠的胎血和成年大鼠的骨髓，用Ficoll-hypague分离液分离出单个核细胞后，种植于含10%胎牛血清的DMEM/LG培养液内。收获的NASC传代培养，用流式细胞仪检测细胞的免疫表型。

CD34+ cells were harvested from G-CSF mobilized adult blood and umbilical cord blood and incubated onto fibronectin/gelatin-coated Petric dishes in the presence of recombinant human vascular endothelial cell growth factorand basic fibroblast growth factor.

毛宁为证明出生后血液循环中存在内皮细胞的前体细胞，从G-CSF动员的成人外周血及脐带血中分离CD34+细胞，将之接种于纤连蛋白与明胶铺板的培养皿上，培养体系中加入重组人内皮细胞生长因子和重组人碱性成纤维细胞生长因子。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

Lugalbanda was a god and shepherd king of Uruk where he was worshipped for over a thousand years.

Lugalbanda 是神和被崇拜了一千年多 Uruk古埃及喜克索王朝国王。

I am coming just now,' and went on perfuming himself with Hunut, then he came and sat.

我来只是现在，'歼灭战perfuming自己与胡努特，那麼，他来到和SAT 。