血培养

Autologus CIK cells were expanded in 1000 ml cluture-bag and reinfused back. The MTT method was used to test the cytoto xicactivity of CIK cells before and after reinfusion.

采用1 000 m l培养袋大量扩增患者自体CIK细胞，用MTT法检测CIK细胞杀伤活性，比较回输前后患者外周血单个核细胞对靶细胞的杀伤活性及其毒副作用。

METHODS: Female BALB/c mice were treated with CCK-8 and keyhole limpet haemocyanin, splenocytes were acquired and incubated in vitro with KLH restimulation, the productions of Th1 cytokines, interferon-γ, interleukin-2 and Th2 cytokines, IL-4, IL-5 in cell culture supernatants were detected by enzyme-linked immunosorbnent assay. The mRNA expressions of IFN-γ, IL-2, IL-4 and IL-5 in splenocytes were detected by reverse transcription-polymerase chain reaction. The levels of Th1 antibody IgG2a and Th2 antibodies IgG1 in serum were also detected by ELISA.

给予BALB/c小鼠钥孔戚血蓝蛋白免疫同时体内给予不同剂量的CCK-8，酶联免疫吸附试验检测其脾细胞培养上清中Th1型细胞因子γ-干扰素、白细胞介素-2（IL-2）和Th2型细胞因子白细胞介素-4（IL-4）、白细胞介素-5（IL-5）水平，逆转录聚合酶链式反应法检测脾细胞中IFN-γ、IL-2、IL-4、IL-5 mRNA表达；ELISA法检测血清中Th1型抗KLH抗体IgG2a和Th2型抗KLH抗体IgG1水平。

Methods Pregnant rats were gavaged castor oil-diet in 18 and 19 days of gestation. At the time of death, blood of portal and peripheral veins, intestinal mucosa, amnion, amniotic cells and placenta were collected, and the tissues were cultured in the presences of ricinoleic acid or indomethacin.

将妊娠18天的Wistar大鼠分实验组和对照组各8只，实验组大鼠每天两次灌服引产餐，灌服4次后剖杀大鼠，收集门静脉、末梢血，胎盘、羊膜组织及制备羊膜细胞进行体外培养。

Methods Hepatoma carcinoma cells and pulmonary carcinoma cells were cultured in vitro, Mononuclear cells in healthy person's peripheral blood were collected. PBMCs were mixed with liver cancer and lung cancer cells by proportion, and enriched tumor cells by superparamagnetic nanoparticle antibody complex, lung cancer specificness was identified by fluorescence nanocrystals bioprobe. We smeared tumor cells on glass slide and identified pulmonary carcinoma cells specfficity by fluorescence nanocrystals bioprobe.

体外培养人肝癌细胞和肺癌细胞，采集健康人外周血单个核细胞，将PBMC与肝癌细胞和肺癌细胞按比例混合，通过磁粒抗体复合物富集癌细胞，用荧光纳米晶探针进行肺癌特异性鉴定。

Cultivation results of blood,sputum,dejecta,uria,abdominal draining juice and clinic data were collected from 129 systemic fungal infection patients.

方法收集129例ICU深部真菌患者的血、痰、粪便、尿、腹腔引流液培养结果及其临床资料。

In conclusion, our results showed urinous supernatants should be used as candidate to diagnose candidemia by PCR.

综上所述，我们证实在血浆中存在游离DNA，同时发现在尿上清中也存在游离DNA。以前的研究证实尿培养可能对念珠菌血症无诊断意义。

Among them group A dendritic cell surface processes were less and cytoplasm vacuolated obviously. 2. Compared the expression of cell surface molecules of 5d, 7d ,10d,14d, we found the CD1a, CD83, CD86, HLA-DR and CD80 expression was significantly increased P.

口腔癌患者外周血单个核细胞诱导培养第5d、第7d、第10d、第14d时，细胞表面DC分子的表达情况：第7d时的DC与第5d相比，CD1a、CD83、CD86、CD80和HLA-DR表达率明显上升P 0。

The cells were then maintained in culture flasks and further identified by immunofluorescence,immunocytochemistry,and vasculogenesis experiment.

结果兔外周血单个核细胞体外培养可成功获得内皮祖细胞，稳定表达内皮祖细胞相关抗原，并能在matrigel凝胶上形成稳定的血管腔样结构。

The molecular approach to detecting peptide hormones using cDNA probes should also be much faster than the immunological method because it can take years of tedious purifications to isolate peptide hormones and then develop antiserums to them.

采用cDNA探子来测定肽激素的这一分子生物学方法同时也应该比免疫学的方法速度来得快，因为对于免疫的方法来说，需耗费好几年枯燥的提纯进程，方能将肽素分离了出来，然后再培养出针对它们的抗血难句类型：复杂修饰、易混指代

objective to investigate the methods of culturing the dendritic cells from human peripheral blood mononclear cellsand its influencing factors.

目的 探讨人外周血树突状细胞（dendritic cells，dcs）的体外培养方法及其影响因素。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。