血培养

The PCR based assay was tested on 19 strains isolated in throats from patients with guttate psoriasis. The results demonstrated that 6 strains were positive in 15 streptococus strains among which there were 3 streptococus pyogenes, 1.S.agalactiae, 1 S. eguisimilis and 1 S. uberis, and 4 non- streptococus strains were negative. Emm6 was found not only in suppurative hemolytic streptococus, but also in S.uberis.

应用聚合酶链式反应方法对点滴型银屑病患者咽部培养出的19株细菌中的M6蛋白基因进行检测，结果，15株链球菌中6株为阳性，其中化脓链球菌3株，无乳链球菌、似马链球菌和乳房链球菌各1株，4株非链球菌均为阴性。M6蛋白基因不仅存在化脓性溶血链球菌中，在其它链球菌如乳房链球菌中也存在。

The juvenile hormones structure, synthesis site and physiological functions of Haemaphysalis longicornis were investigated using tissue culture, biochemistry, RCA, LSS, GC and GC/MS.

运用组织培养、生物化学、放射化学分析、液闪和气相色谱/质谱技术对长角血蜱保幼激素化学结构、合成位点和生理功能进行了研究。

For Streptococcus penumoniae、Hemophilus in0fluenzae、Staphylococcus aureus、Acinetobacter baumannii、Pseudomonas aeruginosa,the sensitivety of Tem-PCR was 100%、41.7 %、11.1%、50.0 %、66.7 % respectively; the specificity was 84.0%、81.7%、98.9%、95.2%、98.9% respectively. Tem-PCR was more sensitive than culture in the detection of Streptococcus penumoniae 36/19.1% vs.

3以细菌培养作为参考标准，Tem-PCR对14种病原菌总的敏感性为51.0%，特异性为68.0%，符合率为58.3%，对肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、鲍曼氏不动杆菌、铜绿假单胞菌的敏感性分别为100%、41.7%、11.1%、50.0%、66.7%，特异性分别为84.0%、81.7%、98.9%、95.2%、98.9%。

Methods Under aseptic condition, mechanically blocking of the hepatoduodenal ligement was adopted in rabbit model, both endotoxin level of the portal blood and positive bacterial cultures in the mensenteric lymph nodes were observed at 15, 20, 30 and 60 min respectively after the blocking.

采用兔动物模型，在无菌条件下机械性阻断肝十二指肠韧带，观察阻断后15，20，30min和60min时门静脉血中内毒素值和肠系膜淋巴结细菌培养阳性数。

We aim to screen and identify the key potential trans-factors during hemoglobin switch. We firstly analyzed differential expression of mRNAs in erythroid induction cultures of CD34+ cells derived from normal umbilical cord blood, adult bone marrow, and bone marrow of a heterocellular hereditary persistence of fetal hemoglobin patient. We identified ZF (HCF-binding transcription factor, Zhangfei) and SH3GLB1(SH3-domain GRB2-like endophilin B1) that had differential expression in the above three cultures. Furthermore, we confirmed the different expression of the above two genes by quantitative real-time PCR.

为鉴别影响珠蛋白基因表达和开关的新的重要调节因子，我们首先分析了人脐带血、正常成人骨髓和异细胞型胎儿血红蛋白持续存在综合症患者骨髓细胞红系诱导培养物中基因表达的差异，发现ZF(HCF-binding transcription factor，Zhangfei)和SH3GLB1（SH3-domain GRB2-like endophilin B1）在这三种不同来源的红细胞内具有显著的表达差异，并通过实时定量PCR方法验证了差异的真实性。

In this study, Mono-nuclear cells containing hematopoietic stem/progenitor cells were isolated from umbilical cord blood of normal full-term deliveries, healthy adult and heterocellular HPFH patient bone marrow by density gradient centrifugation. After simple purification and expansion in vitro, MNCs were induced to erythroid cell differentiation using different culture systems and cytokines including Epo, IL-3 and GM-CSF.

本研究首先利用密度梯度离心方法，从正常成人和异细胞型遗传性胎儿血红蛋白持续存在综合症家系患者的骨髓组织、健康足月正常分娩胎儿脐带血中分离得到了含有造血干／祖细胞的单个核细胞(Mono-Nuclear Cell，MNC)，进行初步纯化及体外扩增后，组合使用Epo、IL-3、GM-CSF等细胞因子，将造血干／祖细胞向红系方向进行诱导培养。

Methods Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of hepatocellular carcinoma patients.Tumor associated antigen extracted from human liver cancer HepG2 cell line was used to activate the DC,which will initiate homogenic T lymphocyte to form cytotoxic T lymphocyte.HepG2 cells were transplanted to the nude mice and were treated with CTL.Expression of PCNA in tumor cells was evaluated.

联合应用粒/巨细胞集落刺激因子及白介素-4(IL-4)直接从肝癌患者外周血中培养出DC；以源于人肝癌细胞系HepG2细胞的肿瘤抗原粗提物刺激DC；DC激活同源的T淋巴细胞产生细胞毒性T淋巴细胞；建立人肝癌细胞系HepG2裸鼠移植瘤模型；CTL治疗人肝癌细胞系HepG2裸鼠移植瘤；检测移植瘤标本肿瘤细胞PCNA表达水平。

Methods: Mice were inoculated with tumor cells. Inhibitory effect of transfection with pCH510 on murine tumor origined from different inoculative dose and inhibiting effect of immediate transfection pCH510 after chemotherapy on tumor were observed, respectively. By cell culture technique, the influence of chemotherapeutic drug to activation of marcrophages and lymphocytes after i.p. injection of drug was observed. By cell counting method, the kinetics of the change of number of peripheral blood immunocytes induced by administration of chemotherapeutic drug was observed. The inhibitory effect of transfection with pCH510 five days after chemotherapy on murine tumor was observed.

采用瘤细胞接种建立小鼠肿瘤模型：通过基因转染，观察pCH510对不同接种量所形成的小鼠肿瘤的抑制作用以及小鼠肿瘤化疗后立即进行pCH510转染的抑瘤效果；采用细胞培养技术，观察小鼠体内注射化疗药物后，其对小鼠腹腔巨噬细胞和脾淋巴细胞激活功能的影响；采用细胞计数的方法，观察化疗药物所致小鼠腹腔巨噬细胞和外周血免疫细胞数量变化的动力学；另外小鼠肿瘤化疗后第5天进行pCH510转染，观察抑瘤效果。

VLDL can downregulate the expression of the hOAT1gene possibly induced by lipodystrophy easily acting with hyperuricemia.

VLDL可下调hOAT1mRNA的表达，可能与脂代谢紊乱易合并高尿酸血症有关。脂蛋白类，VLDL；尿酸；肾小管；细胞，培养的；基因表达

NO.1 chromosome was metacentric, while that of quail was submetacentric.

采用外周血淋巴细胞培养法和胰酶处理法，对家鸡和鹌鹑染色体的核型和G带进行了研究。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。