血培养

The results showed as follows: The diploid chromosome number of Yanbian yellow cattle and Liyan hybrid cattle were 2n=60,the karyotype of bulls was 2n=60,XY,while cows were 2n=60,XX. 29 pairs of autosomes were telocentric.

采用外周血淋巴细胞培养技术、常规染色体制片技术和染色体C-分带技术，对延边黄牛(10♀，10♂)和利延杂交牛(14♀，15♂)两品种的染色体核型和C-带进行了研究。

Objective: To study the effect of Shuxuetong injection on cerebral tissue and brain microvascular endothelial cell in secreting tissue-type plasminogen activator.

中文摘要：目的：探讨疏血通注射液对在体和体外培养的脑组织微血管内皮细胞(brain microvascular endothelialcell，BMEC)分泌组织型纤溶酶原激活物(tissue plasminogeon activator，tPA)的影响。

Results HA titer of two Lanzhou strains cultured in chicken embryo was high up to 1∶512.

结果 2株兰州株在鸡胚中培养血凝效价滴度高达1∶512。

Methods:Pigspleen cells were cultured with HBvaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator;the homogenate was diluted with distilled water to 1∶1, then frozen and melt six times at25 ℃ before placed in the dialyzer at the melting state , after that dialyzed with 1∶4 distilled water as the outdialyzate at 4 ℃ for 48 h;the final outdialyzate was collected as the HBVSTF.

采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1∶1稀释，－25 ℃反复冻融6次，自来水融化后装入透析袋中，用1∶4的蒸馏水作透析外液，于4 ℃透析48 h，透析液即为乙肝病毒特异性转移因子。

Methods: Pig-spleen cells were cultured with HB-vaccine and phytohaemagglutinin in which the sensitized cells were collected and homogenized to pieces by a constitution triturator; the homogenate was diluted with distilled water to 1:1, then frozen and melt six times at -25℃ before placed in the dialyzer at the melting state, after that dialyzed with 1:4 distilled water as the out-dialyzate at 4℃ for 48 h; the final out-dialyzate was collected as the HBV-STF.

方法；采用猪脾细胞与植物血凝素、乙肝疫苗共同培养，收集致敏细胞在组织捣碎机中破碎，用蒸馏水1：1稀释，-25℃反复冻融6次，自来水融化后装入透析袋中，用1：4的蒸馏水作透析外液，于4℃透析48h，透析液即为乙肝病毒特异性转移因子。

In vitro,we can harvest a lot of megakaryoctes derived from cord blood in the new two-step method.

在体外，使用种新的两步培养法可获取大量的脐血来源的巨核细胞以供研究使用。

To isolate mononuclear cells from foetus umbilic cord blood, After MNCs were incubated in the presence of APS group (APS 200 mg·L-1) and control group for 24 h,the cells were stimulated with Epo（5 IU/mL） for 0,2,5,30 min; STAT5 was measured by ICC and laser confocal scanning microscope, JAK2、STAT5 in nucleus and cytoplasm were measured by western-blotting-ECL in each group at different groups.

分离纯化胎儿脐带血单个核细胞，在常规体外培养体系加入APS（200 mg·L-1）作用细胞24 h，促红细胞生成素分别刺激0，2，5，30 min，免疫细胞化学与激光共聚焦显微镜观察细胞STAT5的表达；Western-blotting-增强化学发光法检测细胞JAK2与浆和核中STAT5的表达。

OBJECTIVE: To isolate MSCs from human umbilical cord blood, and to detect their biological properties.

目的：拟从人脐带血中分离培养间充质干细胞，并对其生物学特性进行检测。

To explore the optimal condition of isolation and cultivation of mesenchymal stem cells derived from human umbilical cord blood.

中文摘要目的： 1、探讨人脐血间充质干细胞体外分离培养的最佳方法。

So it is concluded that Vibrio parahaemolyticus 1211U can enter the viable but nonculturable state under certain conditions.

本实验证明了副溶血弧菌1211U株在一定的条件下可进入活的非可培养状态。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。