血培养

OBJECTIVE: To explore the method to isolate and culture EPCs from porcine peripheral blood in vitro.

目的：拟在体外建立猪外周血内皮祖细胞的分离培养方法。

Eventually we might be able to take T regs out of cord blood in order to have a source from which to grow more.

最终，我们可能会把T调节细胞从脐带血中提取出来作为培养源以便生成更多的T调节细胞。

The "regulable osteoinduction microcirculation/microenvironment system in vivo" has characteristics as follows: the culture conditions are controlled. For example, the release of a variety of growth factors can be controlled stably and quantitatively at bone defect site. Early nutrition and revascularization in the center of tissue-engineered bone can be effectively solved; some shortcomings resulted from construction of tissue-engineered bone in vivo, including secondary injury, high level technical requirements, and complex operation, can be avoided; vascularization and osteogenesis can be constructed simultaneously; simple safe and efficient operation.

可调控的体内灌注诱导成骨微循环系统具有以下特点：可以控制诱导培养条件，如调控各种生长因子微量稳定的缓控释放；能有效解决大块组织工程骨中心部位的早期营养和血运重建难题；避免了组织工程骨体内构建存在的二次损伤，需要较高的技术条件、操作较为复杂等缺陷；实现血管化与成骨的同步构建；操作简便，安全高效。

SCAP easy occurred in the aged people. The pathogen of sputum included 76.0% of gram- negative bacteria and 24.0% of gram- positive bacteria.

1.3.3 细菌分离和鉴定：合格的痰标本接种在血平板上，35℃培养后，按常规方法分离，并采用法国生物梅里埃公司VITEK- AMS鉴定。

In fact, rechallenge of T cells with CB CD34+ cells, or immature monocyte-derived dendritic cells in secondary MLC induced potent T cell proliferative responses.

事实上，再次激活的T细胞与脐带血CD34+细胞，或不成熟的单核细胞来源的树突状细胞在次级混合淋巴细胞培养下可以诱导有潜能的T细胞增殖反应。

Methods The lymphocytes of rats were isolated from the peripheral blood and cultured with sodium arsenite under 37 ℃.The ROS content was detected by DCFH-DA;The content of LPO was detected by fluoresce method.

体外分离大鼠外周血淋巴细胞后，施加处理因素，在37℃条件下恒温培养，用2'7'-二乙酰二氯荧光素染色法检测细胞内的ROS水平，用硫代巴比妥酸荧光法测定细胞内LPO含量。

The counsel for the defence had spoken tolerably well, in that provincial tongue which has long constituted the eloquence of the bar, which was formerly employed by all advocates, at Paris as well as at Romorantin or at Montbrison, which to-day, having become classic, is no longer spoken except by the official orators of magistracy, to whom it is suited on account of its grave sonorousness its majestic stride; a tongue in which a husband is called a consort, a woman a spouse; Paris, the centre of art civilization; the king, the monarch; Monseigneur the Bishop, a sainted pontiff; the district-attorney, the eloquent interpreter of public prosecution; the arguments, the accents which we have my.ssbbww.com listened to; the age of Louis XIV., the grand age; a theatre, the temple of Melpomene; the reigning family, the august blood of our kings; a concert, a musical solemnity; the General Commandant of the province, the illustrious warrior, who, etc.; the pupils in the seminary, these tender levities; errors imputed to newspapers, the imposture which distills its venom through the columns of those organs; etc.

那个辩护士谈得相当好，他那种外省的语句，从前无论在巴黎也好，在罗莫朗坦或蒙勃里松也好，凡是律师都习惯采用，早已成为律师们的词藻，但今天这种语句已成古典的了，它那种持重的声调、庄严的气派，正适合公堂上的那些公家发言人，所以8ttt8现在 dd dtt。 com 只有他们还偶然用用；譬如称丈夫为"良人"，妻子为"内助"，巴黎为"艺术和文化的中心"，国王为"元首"，主教先生为"元圣"，检察官为"辩才无碍的锄奸大士"，律师的辩词称"刚才洗耳恭听过的高论"，路易十四的世纪为"大世纪"，剧场为"墨尔波墨涅殿"，在朝的王室为"我先王的圣血"，音乐会为"雍和大典"，统辖一省的将军为"驰名的壮士某"，教士培养所里的小徒弟为"娇僧"，责令某报该负责的错误为"在刊物篇幅中散布毒素的花言巧语"等等。

Methods: The lymphocyte,marrow and spermary which are cultivated outside the body of Guenther are used as the experiment material.

以离体培养的外周血淋巴细胞、骨髓和精巢为材料，按常规的秋水仙素-低渗-空气干燥制片法制片和组型分析。

Results After induction by VEGF or PDGF for 1 week,most of adherent mononuclear cells became round and small, while few grew into spindle-shaped cells.

结果新鲜脐血分离单个核细胞培养1周后贴壁细胞的EPCs特异的Dil标记乙酰化低密度脂蛋白鉴定为80％阳性。

Junshan pharmaceutical adhering to the "carry forward the Sinopharm culture, relying on 800 years of natural medicine Funiushan resources to do the modern Chinese medicine, committed to human health" business purposes, the use of modern production technology and modern management mode, using the world's leading low-temperature supercritical CO2 technology extraction, macroporous resin adsorption separation and cell culture techniques of traditional Chinese medicine active ingredients Road enrichment extraction, continuous extension of drug efficacy, has successfully developed "Wuzi Yanzong pill","Tim Mei Bushen Yangxue day pill,"" Shuanghuanglian tablet "," Xiaoshi stomachic movie "and many other national brands and more than 30 OTC products, the formation of a unique chain-type product line.

君山制药秉承"弘扬国药文化，依托八百里伏牛山天然中药资源，做现代中药，致力于人类健康"的企业宗旨，运用现代化的生产工艺和现代管理模式，采用国际领先工艺CO2超临界低温萃取、大孔树脂吸附分离及细胞培养等技术，对传统地道中药的有效成分进行浓缩提取，不断延伸药品疗效，先后成功研发"五子衍宗丸"、"天添美补肾养血丸"、"双黄连含片"、"消食健胃片"等多个全国名牌产品及30多个OTC产品，形成了独具特色的链条式产品系列。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。