血培养

In order to detect the expression of N5, N10-methylene-tetra hydrofolate reductase gene in Epstein-Barr virus transformed human B lymphoblast, as well as the cDNA sequence, human peripheral blood was transformed by EBV to build B lymphoblast cell line. Total RNA was isolated from the cell culture. Variant segments of MTHFR cDNA were amplified using RT－PCR protocol and analysed by polyacrylamide gel electrophoresis.

为检测用EB病毒转化建立的永生性人B淋巴母细胞株中N5，N10－亚甲基四氢叶酸还原酶基因的表达及其cDNA序列，用EB病毒转化人外周血B淋巴细胞，建立永生性细胞株后，从培养细胞中提取总RNA，用RT-PCR法扩增MTHFR基因cDNA的不同片段，进行PAGE电泳分析和cDNA序列测定。

Objective:To compare and observe the effects of mixed cord blood plasma and adult AB serum on CD3AK cells culture.

目的：观察比较混合脐血血浆和成人AB血清在CD3AK细胞培养中的作用效果。

Suggested that the effects of mixed cord blood plasma on CD3AK cells culture were similar to that of adult AB serum.

混合脐血血浆在CD3AK等免疫效应细胞的培养中可能和成人AB血清具有相似的效果。

Objective To find a way by which dendritic cells from healthy human monocyte in peripheral blood might be induced to be matured.

目的 建立在体外从健康成人外周血单核细胞诱导培养成熟的树突状细胞的方法。

Objective To study the method for generation largescale mature dendritic cells from nonproliferation progenitors in patient's blood.

目的 探讨从患者外周血树突状细胞前体培养足够数量的树突状细胞及调控其成熟的方法。

In contrast, DBA/2 mice from re-infected with PbANKA showed the high level of parasitemia and the same as that of primary infection; the level of IFN-γ in spleen cell super natants significantly increased on day 4 pi compared with control group (P.01); however, the level of specific IgG in serum didn't obviously elevated (P.01), and all mice died.

相比，PbANKA再感染小鼠出现高水平的原虫血症，与其初次感染水平柯近，再感染后第4d脾细胞培养上清中IFN-γ水平出现明显升高(P.01)，而血清中特异性的IgG抗体水平却无明显变化，与正常小鼠水平接近，小鼠全部死亡。

Results In the 128 specimens, anaerobic bacteria were the main pathogene and mined bacterial infection was the most common feature of spreading odontogenic infection. The predominant bacteria were Prevotella, Porphyromomas, Fusobacterium species, Capnocytophaga, Actionomyces and Eubacterium. Oral strepeococcus group was the most common facultatively anaerobic bacteria.

结果 128例牙源性间隙感染可培养的优势厌氧菌有普氏菌、牙龈卟啉单胞菌、梭杆菌、二氧化碳噬纤维菌、消化链球菌和放线菌；需氧菌和兼性厌氧菌有口腔链球菌、嗜血菌、金黄色葡萄球菌和奈瑟菌。

UCB mononuclear cells with Petri dish culture were served as control.

以同条件平面培养的脐血单个核细胞作为对照。

Methods Core b1od mononuclear cells were isolated by Ficoll, Cd34(superscript +) cells were purified from mononuclear cells by MACS and then cultured in a complete medium containing rhIL-4, GM-CSF, rhSCF, rhFlt-3 Ligand, TGF-β1 and TNF-α. After 14d of incubation, the morphology of the cells was observed, the phonotype was examined and the IL-12 level in the medium was measured.

Ficoll分离脐血单个核细胞，免疫微磁珠法分离纯化CD34细胞，以rhIL-4、GM-CSF、rhSCF、rhFlt-3 Ligand、TGF-β1，及TNF-α体外诱导培养14d，进行形态学观察、表型检测及上清液IL-12测定。

Methods The subjects were divided into two groups: PIH group (n= 15) and normotensive control group (n=15). Peripheral lymphocytes of the subjucts were separated by Ficoll-Hypaque density centrifugation. The lymphocytes were cultured in RPMI-1640 medium with or without pokeweed mitogen for ten days.Then the supernatant was collected and the IgG and IgM were measured by enzyme linked immunosorbent assay.

采用密度梯度离心法分离妊高征组(15例)和正常妊娠组(15例)的外周血B淋巴细胞，并在有或无美洲商陆有丝分裂原存在的情况下培养10天后，收集上清液，采用酶联免疫吸附试验测定每1×106个B淋巴细胞所分泌的IgG和IgM的量。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。