血培养

AIM: Peripheral blood mononuclear cells were cultured in vitro to study the effect of gossypol, a polyphenolic antifertility agent, on the activation of normal human T cells.

目的：以体外培养的人外周血单个核细胞为材料，研究多酚类抗生育药物棉酚对正常人外周血T淋巴细胞体外活化作用的影响。

Once the cells reached fluences of FBS coating on adhesive rats of MSCs: Some of the purified HUCB MSCs at logarithmic phase were growth curve of MSCs: The growth of cells were observed by phase contrast microscope (OLYMPUS CK40), and were detected by EPICS-ALTRA flow cytometry.

免疫表型：取扩增第5代的脐血间充质干细胞，用EPICS-ALTRA流式细胞仪进行检测。主要观察指标：①观察高糖组和低糖组细胞的生长状态。②观察不同时间点胎牛血清包被组与未包被组细胞的贴壁情况并分别计算贴壁率。结果：本实验总共培养和分析了70例脐血间充质干细胞样本。

Although antiHPCA-2 -FITC-positive cells per microliter cord blood correlated with that of Tuk3-positive cells (n=8, r=0.875, P＜0.05), the former correlated with CFU-GM, BFU-E, CFU-Mix and CFUs, and the later only with CFU-GM and CFUs.

分析结果进一步证实外周血含有一定数量的祖细胞，体外培养集落及CD34阳性细胞均低于脐血和骨髓，在比较两种CD34单抗(抗HPCA-2-FITC和Tuk3)中，前者更适合临床和实验研究使用。

The pilus revealed a good growth in Minca medium at 37℃ with a special blood coagulation spectrum formed by agglutinating RBC of human and racoon dog.

该菌毛在Minca培养基上37℃培养能良好表达，具有能稳定地凝集人及貂红细胞的特定血凝谱，血凝作用可被相应抗血清所抑制。

Methods Four hundred samples of rhinopharynx secretion from children 3～5 years old were cultured with special chocolate and blood medium plate. Sp and Hi isolated were tested for antibiotic susceptibility.

方法采用专用巧克力和血平板培养基对采集的400名3~5岁儿童鼻咽分泌物培养并鉴定，对鉴定明确的肺炎链球菌和流感嗜血菌进行不同抗生素检测。

Objective: To confirm the hypolipidemic therapy effect of XIEZHIPING whichcomposed of Alisma Rhizoma the principal drug and probe the mechanismof hypolipidemic action from the view of animal model experiment in vivoto cell culture in vitro, and finally establish the base of new naturalhypolipidemic product by the way of experiment on the golden hamsterhyperlipoidemia animal model and HepG2 cell line culture.

目的： 通过对金黄地鼠高脂血症动物模型及HepG2离体细胞培养的实验研究，证实以建泽泻为君药的纯中药组方—&泻脂平&的可靠降脂作用，并进一步从细胞分子水平初步探讨其降脂作用机理，为研发治疗高脂血症的有效天然药物奠定基础。

To confirm the hypolipidemic therapy effect of XIEZHIPING which composed of Alisma Rhizoma the principal drug and probe the mechanism of hypolipidemic action from the view of animal model experiment in vivo to cell culture in vitro,and finally establish the base of new natural hypolipidemic product by the way of experiment on the golden hamster hyperlipoidemia animal model and HepG2 cell line culture.

通过对金黄地鼠高脂血症动物模型及HepG2离体细胞培养的实验研究，证实以建泽泻为君药的纯中药组方—&泻脂平&的可靠降脂作用，并进一步从细胞分子水平初步探讨其降脂作用机理，为研发治疗高脂血症的有效天然药物奠定基础。

The result showed that the HA titer of virus in allantoic fluid reached its maximum 1:256~1:512 in the condition that the 9-day chick embryo received 50 times dilution of 1:64 GPMV and cultured for 48~72 h in 37℃.

结果表明将血凝价为1：64的鹅副粘病毒稀释50倍后，接种9日龄鸡胚，37℃培养48~72 h获得的尿囊液血凝价可达1：256~1：512，平均尿囊液量可达9.4 mL/胚。

In this study, different nano-hydroxyapatite particles,HAP_1(25-60nm), HAP_4(the additives is heparin, 15-50nm), HAP_5(the additives is bovine serum albumin BSA, 20-80nm) were prepared by homogeneous precipitation and used heavy-gauge hydroxyapatite as comparison,we determined amount of heparin, sialic acid ,BSA adsorbed on HAP_1,HAP_2,HAP_3 by Crystal Violet assay, Bialsche method,Bradford method respectively and analyzed the binding mechanism by infrared spectrum;After taking HAP_1,HAP_2,HAP_4,HAP_5 and RBC to co-culture in vitro,we studied RBC hemolysis test and detected RBC hematolysis rate by erythrocyte osmotic fragility test;observing the changes of morphous and locomotion of cell after coacting HAP_1,HAP_2,HAP_4,HAP_5 and RBC by light microscope and inverted phase contrast microscope;observing HAP_1,HAP_2,HAP_4,HAP_5 effecting on Ultrastructure of RBC.

本文用均匀沉淀法制备了HAP_1(25-60nm)，HAP_4(添加剂肝素，15-50nm)，HAP_5(添加剂牛血清白蛋白BSA，20-80nm)等纳米粒子，并用大尺寸的羟基磷灰石HAP_2(470-520nm)，HAP_3(1906nm)作对照，分别利用结晶紫法、Bialsche法、Bradford法研究了肝素、唾液酸、血清白蛋白在HAP_1，HAP_2，HAP_3上的吸附量，用红外光谱分析其中的结合机理；在体外将HAP_1，HAP_2，HAP4_，HAP_5与红细胞共培养，进行了红细胞溶血试验的研究，并借助红细胞渗透脆性试验检测红细胞溶血率；运用普通光学显微镜和倒置相差显微镜观察了HAP_1，HAP_2，HAP_4，HAP_5与红细胞作用后细胞形态及运动的变化：透射电镜观察了HAP_1，HAP_2，HAP_4，HAP_5对红细胞超微结构的影响。

Methods From Feb 2006 to Jun 2006,188 hospitalized children in Shenzhen children s hospital, were collected deep tracheal aspirate at the time of hospitalization. The respiratory tract secretions were immediately sent for bacterial culture with 3 kinds of medium:ordinary medium, Hemophilus influenzae selective medium, Streptococcus penumoniae selective medium. Then we extracted the total nucleic acids from secretions, and detected Mycoplasma pneumoniae by single fluorescent quantitation PCR. Simultaneously, 14 respiratory tract pathogenic bacterium and Mycoplasma pneumoniae were detected by Target Enriched multiplex PCR. Amplification products were identified by the Luminex100 suspension array.

确诊为社区获得性肺炎的患儿188例，在入院当天采集深部呼吸道吸引物，用普通培养基和肺炎链球菌、流感嗜血杆菌选择性培养基进行细菌培养，然后提取深部呼吸道吸引物中病原体的DNA，采用荧光定量单PCR的方法检测肺炎支原体，并对同一标本采用靶序列富集多重PCR技术同时扩增肺炎链球菌、流感嗜血杆菌、金黄色葡萄球菌、肺炎克雷伯菌、大肠杆菌、嗜肺军团菌、铜绿假单胞菌、鲍曼氏不动杆菌、脑膜炎奈瑟氏菌、阴沟肠杆菌、奇异变形杆菌、化脓链球菌、粪肠球菌及屎肠球菌14种呼吸道病原菌和肺炎支原体的靶基因，扩增产物用Luminex100多功能悬浮点阵仪检测。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。