血培养

Species animalcula were isolated from the wounds of Dracaena cochinchinenis stems. During culture period, the culture mediums or the mycelia of four species among the animalcula turned red, which is in some degree similar to the colour of dragon's blood. They were identified as Fusarium graminearum, and another unknown three species of Fusarium.

从剑叶龙血树茎干损伤部位及叶子斑块部位共分离得到24种微生物，其中有4种在培养时候，培养基或菌体本身不同程度的呈现红色，在一定程度上与龙血竭的颜色相类似，经鉴定，它们是Fusarium graminearm和三种未知镰刀菌属真菌Fusarium sp。。

Methods Retrospective analysis of the clinical data of 116 cases with hospital acquired respiratory tract infection by Haemophilus parainfluenzae during the period from Aug. 2002 to Jan. 2005 had been done. Used VITEK-60 microorganism autoanalysis system to appraise bacteria and its resistance against various antibiotics was carried out. Nirtocefin test was used to detect β-lactamase.

对2002年8月至2005年1月温州医学院附属第一医院住院的116例医院获得性副流感嗜血杆菌呼吸道感染的临床资料进行统计分析，对送检的痰或咽拭子标本用嗜血杆菌专用巧克力培养基进行分离培养，VITEK-60微生物自动分析系统进行鉴定以及药敏试验，用Nitrocefin纸片法测定被试菌的β-内酰胺酶。

Methods: The total polysaccharide was extracted with distilled water from mycelia of paecilomyces cicadae. The colorimetric MTT was used to analyze the proliferative activity of PBMC, U937 and K562 which were treated with PCPS of 7 concentrations respectively in vitro. ELISA was used to detect the content of hTNF-α, hIFN-γ from supernatant of culturing PBMC that was treated with PCPS for 44 h.

用水提取蝉拟青霉菌丝体总多糖，以MTT法测定不同浓度总多糖对人外周血单个核细胞及白血病细胞株U937、K562增殖的影响，ELISA法测定多糖作用44h后，外周血单个核细胞培养上清液中hTNF-α、hIFN-γ的含量。

Pathogen detection was performed, including hemoculture, urine culture, sputum culture, nose swabs culture, throat swab culture, checking clinically important cytomegalovirus, EB-DNA and mycoplasma in blood, acid-fast bacilli and eumycete culture in sputum.

行病原学检测，包括血、尿、痰、鼻拭子、咽拭子培养，痰涂片+培养查真菌，抽血查CMV、EB-DNA及支原体，痰查抗酸杆菌。

P.01, and there is no significant difference between Tem-PCR and culture for the detection of Hemophilus influenzae 40/21.3% vs.

Tem-PCR对肺炎链球菌的检出率36/188(19.1%)明显高于培养7/188(3.7%)，P＜0.01；对流感嗜血杆菌的检出率40/188(21.3%)与培养24/188(12.8%)之间无明显差异，P＞0.05。

Mononucleated cells were isolated by means of density gradient centrifugation from 20ml peripheral blood which collected from healthy normal donor. The mononucleated cells were planted on the glass coverships or bone slices and cocultured whith UMR 106 cell line, DMEM which contented 1,25 dihydroxyvitamin D3, M-CSF and dexamethasone was used as the culture medium.

从健康志愿者抽取外周静脉血，以密度梯度离心方法获得单个核细胞，在预先种植了UMR106细胞的盖玻片及骨片上，用含1，25_2D_3、地塞米松和M-CSF的DMEM培养液进行培养，而在未种植UMR106细胞的盖玻片上进行培养者作为对照组。

Methods Mononuclear cells of peripheral blood were isolated by Ficoll Hypaque centrifugation from 32 breast cancer patients and plated on 6-well culture plates(106 /ml, 2 ml/well) in RPMI1640 medium supplemented with 10％ heat-inactivated fetal bovine serum, GM-CSF, IL-4, and/or TNF-α. Two h later, nonadherent cells were gently removed and fresh medium was added.

方法采用密度梯度离心的方法，分离32例乳腺癌患者外周血中的单个核细胞，在6孔培养板上(106/ml，2ml/孔)，用含10％热灭火的小牛血清和GM-CSF、IL-4、TNF-α细胞因子的RPMI 1640培养基培养。

Serum containing SSTG was obtained at different time after perorally administrated with different dose of SSTG.The cardiomyocytes were deprived of oxygen and glucose to mimic hypoxia reoxygenation injury and were treated by serum containing SSTG when reoxygenation.LDH content in supernatant was detected after reoxygenation.LDH release suppression rate was used to study the time-effect and dose-effect of serum containing SSTG.

以中药有效组分配伍方剂双参通冠方不同灌胃剂量、药后不同取血时间所得的药物血清为受试药物；培养心肌细胞，进行缺氧复氧实验，复氧同时给予不同双参通冠方药物血清处理，实验结束后取培养上清检测LDH值，以LDH释放抑制率为指标，观察含药血清药理作用强度与体内给药的量效、时效关系。

The third-stage larvae were developed to fourth-stage larvae (the most optimal develop rate was 41%) when cultured in defined complete medium, further, cultured in defined incomplete medium, was examined no develop and a poorly survival rate. When the third-stage larvae were cultured in the defined complete medium under 37℃ and 5% CO2 in air, the larvae were began to develop to the fourth-stage larvae in cultured for 30 days, being enclosed within the sheaths of the third molts of life cycle.

广东住血线虫的第三期幼虫以MEM medium添加胺基酸、脂肪酸及碳水化合物的培养基在37℃含有5％ CO2的无菌培养箱中，培养至第21天已发育至第三期幼虫中期的阶段，第24天已发育至第三期幼虫末期的阶段，第30天开始出现带鞘的第四期幼虫，第36天则观察到虫体明显变长并蜕去外鞘及第三期幼虫特有的眼点消失的第四期幼虫。

The sputum, urine, blood and other drainages were collected to perform the fungal examination after three days of admission every three days.

患者于入院3d后即进行痰、尿、血和能采集的其他体液、引流物及分泌物的培养，每3d采集、培养一次。

I am accused of being overreligious," she said in her quiet, frank manner,"but that does not prevent me thinking the children very cruel who obstinately commit such suicide.""

客人们在卡罗利娜·埃凯家里，举止就文雅一些，因为卡罗利娜的母亲治家很严厉。

Designed by French fashion house Herm è s, this elegant uniform was manufactured in our home, Hong Kong, and was the first without a hat.

由著名品牌 Herm è s 设计，这件高贵的制服是香港本土制造，是我们第一套不配帽子的制服。