血

The female salivary gland proteins were influenced by JH Ⅲ. Treatment on d2 after feeding, 10μg dose increased SG protein level and restrained 136kD protein gene expression. Treatment on d0 after engorgement, 10μg, 50μg and 100μg doses significantly increased the d2 SG protein and 100μg dose made 35kD protein gene expression, 200μg treatment made the 136kD and 192kD proteins absent. This is the first found that JHs regulate arthropoda SG activity.

保幼激素对雌蜱唾液腺蛋白及成分有影响，吸血后2天处理，10 μg剂量使唾液腺蛋白含量明显增加，抑制136kD蛋白的基因表达；饱血当天处理，10μg、50μg和100μg显著提高饱血后2天唾液腺蛋白含量，100μg使35kD蛋白表达，200μg使136kD和192kD的蛋白缺矢；饱血当天处理，1μg和10μg剂量均能显著提高饱血后4天雌蜱唾液腺蛋白含量，高剂量（100μg）抑制136kD蛋白的表达，该结果首次报道了保幼激素对节肢动物唾液腺的调控作用。

A numerical method to estimate hemolysis level in blood pump was presented and hemolysis indices were calculated.

给出了一种利用数值方法计算溶血指标的方法，并通过溶血指标的计算评价了血泵内的溶血程度，发现雷诺切应力是导致溶血的主要原因。

Fig 1 Negative control Female umbilical cord sample has no Y-specific signal after in situ hybridization with the biotinylated Y-repeated sequence DNA probe PY3.4 Fig 2 Positive control Flow-sorted male umbilical cord cells hybridized to Y-specific DNA probe PY3.4,Every cell contains a Y-specific signal Fig 3 Fetal cells sorted from maternal blood Flow-sorted cells from a pregnant woman at 20 weeks of gestation hybridized to Y-specific DNA probe PY3.4,containing a Y-specific signal Fig 4 The result of polymerase chain reaction Lane 1:male umbilical cord NRBCs sorted by FITC-conjugated anti-monoclonal glycophorin A;Lane 2:sample of 2;Lane 3:male umbilical cord NRBCs sorted by FITC-conjugated anti-CD36;Lanes 4-5:samples of 1 and 3;Lane 6:50 cells of male;Lane 7:5 cells of male;Lane 8:200ng male DNA(positive+control);Lane 9:nonpregnant female DNA(negative+control);Lane 10:ΦX174 HaeⅢ Maker,271bp:amplified band of Y-specific gene SRY;383bp:amplified band of human β-globin gene

图1 阴性对照女性脐带血标本，经与生物素标记的Y-染色体重复序列DNA探针原位杂交后未见Y-染色体特异信号图2 阳性对照分选的男性脐血细胞经与Y-特异DNA探针杂交后，每一细胞都含有Y-染色体特异信号图3 母体外周血中分选的胎儿细胞从一位妊娠20周的孕妇外周血中分选出的细胞经与Y-特异DNA探针杂交细胞中含有Y-染色体特异信号图4 聚合酶链反应结果 1：GPA-FITC单抗分选男胎脐血NRBCs；2：2号标本；3：CD36-FITC单抗分选男胎脐血NRBCs；4、5：1、3号标本；6：50个男性细胞标本；7：5个男性细胞标本；8：200ng男性DNA；9：未孕女性DNA；10：ΦX174 HaeⅢ标准，271bp：为SRY基因扩增带；383bp：为人β-珠蛋白基因扩增带内参照

1hyperuricemia subjects have greater risk for high lipemia, high blood sugar,hypertension, artherosclerosis, obesity,and adomen-obesity. 2Beer was a risk factor, but hyperuricemia has not relation with education degree and smoking. 3To prevent and to therapia hyperuricemia need control weight,below blood pressure and lipemia., sufficient movement, blank diet, control drinking.

1高尿酸血症常伴发高血压病、动脉粥样硬化、高脂血症、高血糖、腹型肥胖。2啤酒是促进高尿酸血症发病的重要的危险因素，但文化程度、吸烟与血尿酸水平的相关性无统计学意义。3预防和治疗高尿酸血症必需控制体重、降低血压和血脂，适量运动，清淡饮食，控制饮酒。

To evaluate the interaction effects of the three chemical components, Cochinchinenin A, Cochinchinenin B and Joureirin B, of dragon's blood resin, a new method of isobologram on the basis of the Loewe Additivity was established and could analyze the interaction effect of the three drugs with variable potency rations. By using this new method we assessed the interaction characteristic of the combined inhibition effects of three chemical components of dragon's blood resin on the evoked discharges of wide dynamic range neurons of spinal cord in rats.

为了评估龙血竭的3种化学成分剑叶龙血素A、剑叶龙血素B和龙血素B在活体动物实验中的相互作用特性，从经典的Loewe Additivity模型中推导出了在效价强度比变化的情况下判断3种药物的相互作用性质的等效图分析方法，以此分析了龙血竭的上述3种化学成分联合抑制大鼠脊髓背角广动力范围神经元诱发放电频率时的相互作用类型。

Le die EM, life-saving care should not be too anxious to force all the blood for more than two thirds in the last rescue, like this one hit the last of blood, half blood to be careful when Le, BOSS will be skills increases blood return to a half-blood, is basically about one seconds SHUNFA luminescence time, then明教, monks, have a good red BOSS immediately points, eight array Xiaoyao also prepared, ready to attack, scattered, EM moonset blind but the blind can also be under the BOSS End or back to Canada and points to the post.

死勒EM，救人也要小心不能太着急要在队伍血全部在3分之2以上在去救人，这个样子，打到最后一管血，半血的时候要谨慎起来勒，BOSS会有个技能加血恢复到一条半血的，基本是顺发大概1秒发光时间，那时候明教，和尚，都要看好BOSS一出红光，马上点，逍遥也准备八阵，准备散攻，EM月落失明下也可以不过失明完BOSS还是回加，点到后。

Will nurse now the introduction is as follows. 1 clinical data this group patient of 58 tuberculosis big haemoptysis, male 38, female 20; age 20 years old of ~75 year old; haemoptysis all exceeds 500mL inside quantity 24h, 10 when exceed 800mL among them, 5 of shock of amalgamative and exsanguine sex, 3 when amalgamative haemoptysis chokes. 58 ill average per capita live in the rescue room of my division, rescue is indoor set major to protect personnel especially, 24h is on duty, did not accompany protect, via be treated actively and nursing, all ill average per capita gets controlling in the 1 haemoptysis inside week. 2 nurse 2.1 after doing good rescue to prepare be in hospital of patient of phthisical big haemoptysis, all arrange in rescue room, interior equipment good oxygen, attract implement, appearance of custody of report of bag of tracheal spile, tracheal incision, heart, synchronous lung ventilator, except quiver the rescue equipment such as appearance and relevant rescue medicines and chemical reagents, if appear,choke wait for critical condition, in order to facilitate rescue. Rescue of 2.2 environments requirement is indoor should maintain air fresh, temperature is appropriate, keep quiet and eliminate all sorts of undesirable stimulation, avoid family member visit, reduce air pollution and alternate infection.

现将护理介绍如下。1临床资料本组58例肺结核大咯血病人，男38例，女20例；年龄20岁~75岁；咯血量24h内均超过500mL，其中超过800mL的10例，合并失血性休克的5例，合并咯血窒息的3例。58例病人均住在我科的抢救室，抢救室内设有专业特护人员，24h值班，没有陪护，经积极治疗和护理，全部病人均在1周内咯血得到控制。2护理2.1做好抢救预备肺结核大咯血病人住院后均布置在抢救室，室内备好氧气、吸引器、气管插管、气管切开包、心电监护仪、同步呼吸机、除颤仪等抢救器材及相关抢救药品，若出现窒息等危急情况，以便于抢救。2.2环境要求抢救室内要保持空气新鲜，温度适宜，保持恬静和消除各种不良刺激，避免家属探视，减少空气污染和交叉感染。2.3。。。

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

Methods 104 patients with chronic cerebral circulation insufficiency were randomly divided into two groups.in basis of routine therapy, patients in treatment group (n=56) were treated by yangxue qingnao granules,4 g,three times a day; patients in control group (n＝48) were treated by troxerutin, 3 pills a day.the course was 30 days in two groups.the indexes of hemorrheology and mca,aca,pca, va, ba and pi were observed before and after treatment.results there were significant differences between two groups in blood viscosity, plasma viscosity, mca,aca,pca,mca and pi after treatment (p.05).conclusion yangxue qingnao granules have definite effect on chronic cerebral circulation insufficiency, and decrease and hemorrheology in patients with ccci, relieve angiospasm and improve clinical symptoms.

将慢性脑供血不足患者104例随机分为2组。在常规治疗基础上治疗组56例加服养血清脑颗粒，每次4 g，每日3次口服。对照组48例在常规治疗基础上加服曲克芦丁，每次2片，每日3次口服。2组均30日为1个疗程。观察2组临床疗效，治疗前后血液流变学指标及大脑中动脉、大脑前动脉、大脑后动脉、左右椎动脉和基底动脉的搏动指数及阻力指数的变化。结果 2组治疗后全血黏度、血浆黏度，mca、aca、pca的pi及mca的ri及总有效率比较差异均有统计学意义(p＜0.05)。结论养血清脑颗粒治疗慢性脑供血不足疗效确切，能降低ccci患者的血液黏稠度及血液流变学指标，增加脑供血，缓解血管痉挛，改善临床症状，改善脑供血不足。

Methods: Picric performance rate method is used to measure anhemolytic samples, factitious hemolytic samples, post-factitious hemolytic samples with 0.88mol/L H2O2 37℃ water bath for 20min, post-factitious hemolytic samples with 0.88mol/L H2O2 37℃ water bath for 30min, anhemolytic samples with 0.88mol/L H2O2 37℃ water bath for 20min to deal with five of the numeral value of creatinine in blood samples.

用苦味酸速率法测定未溶血、人为溶血、人为溶血后加0.88mol/L H2O237℃水浴20m in、人为溶血后加0.88mol/LH2O2 37℃水浴30min、未溶血加0.88mol/L H2O2 37℃水浴20m in五种处理的血液样品中肌酐值。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。