蟾酥

Further more, toad venom is an expensive medicine, the adulterant can be found in commercial products frequently.

我国蟾酥药源丰富，除药典规定的两个品种外，尚有花背蟾蜍和华西大蟾蜍也用来采集蟾酥。

ObjectiveThe aim is to research the inhibiting function and the mechanism of Chansu to the CEM cells.

目的研究蟾酥对CEM细胞的抑制作用及其机制。

Objective To probe the antineoplastic effect and immunoregulatory effect of senso injecta, and to study the preventing and curing untoward effect caused by chemo-treatment treating on those with advanced non-small cell carcinoma of lung.

目的：研究蟾酥注射液的抗肿瘤作用和免疫调节作用，以及防治晚期非小细胞肺癌患者化疗引起不良反应的作用。

The senso injecta not only does not influence the curing effect of chemo-treatment on those with advanced non-small cell carcinoma of lung, but can enhance organism immunizing power and decrease the side-effect of chemo-treatment and improve the patients' living standard, to some degree, improve the patients' tolerance of chemo-treatment.

蟾酥注射液不影响晚期非小细胞肺癌患者化疗疗效，并且能增强机体免疫力、减轻化疗不良反应、改善患者的生活质量，在一定程度上提高机体对化疗的耐受性。

Depression of myocardial contractibility in rats by free fatty acids during hypoxia.

低温及蟾酥对麻醉家兔心肌缺血再灌注损伤的保护作用。

Methods A two stage pressure homogenizer was used to prepare Venenum Dufonis lipid microsphere for injection. The effects of oil phase,emulsifier,pH and sterilization method on the stability of lipid microsphere were investigated separately.

采用两级高压均质法制备蟾酥脂质微球注射液，分别考察了油相组成、乳化剂用量、pH、灭菌方式对制剂灭菌稳定性的影响。

Ningaloo Marine Park, Western Australia - A banded toadfish hides among the coral on 185-mile-long (298 kilometers) Ningaloo Reef.

宁哥路海洋公园，西澳大利亚-以185中的珊瑚带蟾酥隐藏英里长（298公里）宁哥路珊瑚礁。

To produce medicine serum of Liu-Shen-Wan with different dose, different time of pouring medicine, different time of blood sampling.2. To measure content of arsenic in serum with atomic absorption method, to qualitative analysis toadfish in medicine serum of Liu-Shen-Wan with thin layer chromatography.3. MTT and trypan blue assay detects the growth inhibition effect of Liu-Shen-Wan on HL-60 cells and to make certain the best scheme of making method of medicine serum of Liu-Shen-Wan.4. Gimsa dying assay observes morphology changes of apoptosis .In situ end-labeling method and flow cell machine quantificationally probes apoptosis phenomena.

1 分不同的给药剂量、给药天数和不同的采血时间制备含药血清。2 用原子吸收法测定血清砷含量；薄层色谱法定性检测六神丸含药血清中蟾酥成分。3 用MTT法、胎盘蓝染色法观察六神丸对HL-60细胞的增殖抑制作用，确定最佳血清制备方案。4 用吉姆萨染色法观察细胞凋亡的形态学变化，流式细胞仪、原位末端标记法定量检测细胞凋亡程度。

Results1. Content of arsenic in serum raises with dose adding, time of pouring medicine and time of blood sampling prolonging. Thin layer chromatography indicates that there is toadfish in medicine serum of Liu-Shen-Wan.2. The suppressive rate of medicine serum of Liu-Shen-Wan on HL-60 cell raises with adding of dose and time of pouring medicine and prolonging of blood sampling.3. Gimsa dying assay, in situ end-labeling method and flow cell machine all exhibits: HI-60 cells dealed with Liu-Shen-Wan medicine-serum present typical apoptosis morphology.

结果：1 血清砷含量随着给药剂量、给药天数增加和采血时间的延长而升高；薄层色谱表明六神丸含药血清中有蟾酥成分。2 六神丸含药血清对白血病细胞的抑制率，随给药剂量、天数增加和采血时间的延长而升高3 吉姆萨染色、流式细胞仪检测、原位末端标记检测均证明六神丸含药血清能诱导HL-60白血病细胞凋亡。

Bufotoxin collected from Bufo bufo garigarizans was diluted into 4 different kinds of concentration of 4,6, 8 and 10mg/mL as a factor A, and Enterobacter aerogenes original solutions were diluted with LB liquid medium at 1∶104 and 1∶105 as a factor B.

把采自中华大蟾蜍的蟾酥用蒸馏水稀释成4、6、8、10mg/mL共4种浓度，作为因素A，用LB液体培养基将活化的产气肠杆菌原液按1∶104和1∶105稀释，作为因素B，采用二因子方差分析观察了蟾酥对产气肠杆菌抑菌圈直径的影响，并分析了蟾酥浓度与抑菌圈直径之间的相关关系。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。