螺旋菌

Largely unstructured in solution, linear peptides fold into an amphipathic helix upon binding and insert into the target membrane, leading to breakdown of the transmembranal potential and of ion gradients, thus causing leakage of cell contents, resulting finally in cell death.

大多数的抗菌胜肽在水溶液中是呈现松散的结构，但和目标微生物作用时会褶叠形成双性螺旋的结构并插入其膜内，导致破坏膜内外的电位及离子梯度，造成细胞的泄漏，最后导致细胞死亡。

H. pylori is usually spiral-shaped, it can turn from rod form to coccoid shape after prolonged in vitro culture or antibiotic treatment.

幽门杆菌的菌体形态以螺旋杆状为主，但体外培养时间过长或是在抗生素处置的情况下，会造成菌体的形态由杆状变成球状。

Isolates CNR-1 and CNR-2 were from Brassica napus; CNA-1and CRW-1 from Rhododendron simsii and Oxalis corymbosa respectively. All isolates grew well in R-2 liquid medium and exhibited contractive movements. The colonies of all isolates were circinal and grain-like in solid medium. Through electron microscopy, all isolates exhibited helicity during their growth phase. All isolates could pass through a 0.22 μm filtrate membrane and resist to penicillin (2000 U/mL). They must grow in medium with serum.

这4株螺原体在R-2液体培养基中生长良好，都能通过孔径为0.22 mm的微孔滤膜；在R-2固体培养基上呈圆形或颗粒状菌落，菌落直径约50~600 mm；在生长的某个阶段可呈典型的螺旋状，菌体直径为37.04~370.40 nm，长度约0.89~11.88 mm；它们都能利用葡萄糖作为碳源，不能利用尿素；在不含胎牛血清的R-2培养基中，它们都不能生长；菌株CNR-1、CNA-1能强烈代谢精氨酸，而CNR-2和CRW-1不能代谢精氨酸；在氨苄青霉素钠浓度高达2000 U/mL的R-2培养基中，分离菌株生长良好。

The group disposed by Acetylspiramycin doesn"t show parallel results; The strip gray scale of exotoxin A decreased gradually with the increasing dosages of Azithromycin and Roxithromycin, which doesn"t show in group disposed by Acetylspiramycin and group PAO-JP2; The strip gray scale of lasR, detected by RT-PCR, decreased gradually with the increasing dosages of Azithromycin, which doesnt show in group PAO-JP2.Conclusions: There were obvious differences between PAO1 and PAO-JP2 in biology characteristics in vitro.

本研究发现大环内酯类药物在生物被膜培养过程中分别处理PAO1、PAO-JP2，结果提示大环内酯类药物(14、15元环)通过对QS系统的影响可有效抑制铜绿假单胞菌生物被膜的形成；通过QS系统有效抑制铜绿假单胞菌的毒力因子的产生，16元环的大环内酯类药物乙酰螺旋霉未见上述结论；lasR基因的反转录结果提示阿奇霉素可在转录水平抑制QS系统发挥其作用。

Short chain fatty acid, propyl alcohol, methionine and dimethylamide are the possible precursors for spiramycin formation. Addition of 0.6% propyl alcohol into fermentation medium increases the spiramycin productivity by 24%, while addition of short chain fatty acids, methionine, or dimethylamide has a repression effect on spiramycin production.

合成螺旋霉素的前体物质可能有短链脂肪酸、丙醇、蛋氨酸和二甲胺等，在发酵培养基中加入0.6％的丙醇可以使螺旋霉素的发酵单位提高24％，而短链脂肪酸、蛋氨酸和二甲胺对菌体生长和螺旋霉素的生物合成有不同程度的抑制作用。

Background and aims: Helicobacter pylori is a microaerophilic microorganism. Aerophilic environment may lead the death of H. pylori.

幽门螺旋杆菌为一种微嗜氧菌，如果其周遭之氧气浓度过高，便容易产生死亡。

The isolate grew well in R2 liquid medium and could pass through 0.22 μm and 0.45 μm filtrate membranes. The colony was grain-like in solid medium. Through electron microscopy, the isolate YY0801 exhibited helicity during their exponential growth phase. The isolate YY0801 was able to ferment glucose and D-fructose and to catabolize arginine, but did not to hydrolyse urea. The isolate was resistant to ampicillin (2000 U/mL).

分离菌株在R2液体培养基中生长良好，能通过孔径为0.22 μm、0.45 μm的微孔滤膜；在R2固体培养基上呈颗粒状菌落；在对数期呈典型的螺旋状；能利用葡萄糖、D-果糖作为碳源；能强烈代谢精氨酸；不能利用尿素，在含氨苄青霉素钠(2000 U/mL)的R2液体培养基中生长良好。

The plasmid DNA bands were detected clearly by agarose-EtBr gel electrophore sis.Two forms of plasmid DNA molecules,namely covalently closed circular DNA mol e-cules and open circular DNA molecules,were observed under electron microscope.

用琼脂糖-溴化乙锭凝胶电泳分析在两株菌的DNA中鉴别出明显的质粒带，用电镜方法在两株菌的DNA制剂中均观察到共价闭合超螺旋和开放型环状两种构型的DNA分子。

The molecule of antibacterial peptide displays α-helical structure of amphipathicity, which can form the ion-channel to induce the death of pathogen on the protoplasmic membrane of bacterium, eumycete and parasite.

抗菌肽分子呈双亲性的α-螺旋结构可使细菌、真菌、寄生虫的质膜形成离子通道，从而导致这些病原体死亡。

It was seen that the main microflora causing the spoilage of fresh mutton meat consisted of 3 gram-positive bacilli with spore (about 30% of the micro flora), 1 gram-positive coccus (about 2% of the whole microflora), 3 gram-negative spirilla (about 63% of the whole microflora), and 1 gram-negative bacillus (about 5% of the whole microflora). Potassium sorbate, EDTA and nisin were used as preservatives to inhibit the growth of the main strains representative for the spoilage of fresh lamb meat.

对屠宰后羊肉的微生物区系进行了调查，成功地分离出引起冷却羊肉腐败的主要革兰氏阳性菌和阴性菌8种，其中含芽孢的革兰氏阳性杆菌有3种，占整个微生物区系的30％；革兰氏阳性球菌1种，占整个区系的2％；革兰氏阴性螺旋杆菌3种，占整个区系的63％；革兰氏阴性杆菌1种，占整个区系的5％。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。