螺旋体

Objective To clone the flagellar biosynthesis genes fihA, flhB2 and fliR of Leptospira interrogans and construct their prokaryotic expression system Methods Extract genomic DNA from Leptospira interrogans serogroup Icterohaemorrhagiae serovar Lai strain 56601 by phenol-chloroform method and amplify fihA, flhB2 and fliR gene fragments by high fidelity PCR.

目的 克隆问号钩端螺旋体鞭毛相关基因flhA、flhB2和fliR，并构建其原核表达载体。

Because it has high flexibility, it makes operator can arrange all kinds of equipment conveniently. The unique motion part of flectional beating in the transmitting pipe. Therefore the property of machine is reliable. It does not need to maintain nearly and cannot take place the problem of pollution caused by lubricating grease of beating.

挠性螺旋体是该输送机唯一的运动部件，无论做多远距离输送，输送管中没有轴承等其它运动部件，因此该输送机性能可靠，几乎不需要维修，不会发生如周朝呢感润滑油污染输送物料这类问题。

Spirochetes were observed in midguts of 12.0% of 50 Haemaphysalis bispinosa in this area by Direct immunofluorescence test.

当地的蜱类以二棘血蜱为优势种，占85%，该蜱中肠携带莱姆病螺旋体率为12.0%(650)。

The results indicated that GLP was a linear (1→3)β-D-Glcp main chain linkage. Its monosaccharide component was predominantly composed of D-Glc, and small amount of galactose, mannose, xylose and idose, residues of branches terminated with substituted at 1→6 by a small number of singleunit β-D-Glcp side-chains, it's also observed that the (1→3)-linked β-D-glucan contained a triple-helical conformation, which was composed of a repeating unit with a structure as below:.

结果表明：灵芝多糖含有三螺旋体构型，GC-MS分析灵芝多糖的主要单糖组分为葡萄糖，还有少量的半乳糖、甘露糖、木糖和艾杜糖，IR及1HNMR分析多糖为β-构型，HIO4氧化、Smith降解和甲基化分析表明：多糖主要为(1→3)糖苷键连接构型，并伴有少量的1→6位支链键连接的结构，灵芝多糖是由D-葡萄糖单元通过β-(1→3)糖苷键连接葡聚多糖，其主要构型特征为(1→3)β-D-线性连接的骨架结构。

There are many different types of leptospira bacteria, each tends to be associated with a specific animal species.

有许多不同类型的钩端螺旋体菌，每个往往会联想到特定的动物物种。

The data suggest that Leptospira prefers the excitement of the big city.

数据暗示钩端螺旋体更喜欢呆在喧闹的大城市。

This process is optimal for preserving the nutrient factors critical to the applications of cell culture, leptospira and microbiological growth.

这个过程是保存细胞培养，钩端螺旋体和微生物生长中营养因素的最佳方法。

ABSTRACT Aim To rapidly detect Leptospira in ditch and jungle by nested PCR.

摘 要 目的用套式 PCR 快速检测水沟和丛林等地的钩端螺旋体。

To our viewpoint,this method could be applied to rapidly identify and preliminary classify Leptospira interrogans and one of effective molecular epidemiological means.

用此法对钩端螺旋体进行快速鉴定，初步分类，不失为分子流行病学调查的一种有效手段。

Additionally , The presence of other infections such as PRRS and leptospira may increase the severity of disease.

此外，如果猪群中存在繁殖呼吸综合症或钩端螺旋体病，则本病病情会加重。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。