螠

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我们还用RT-PCR方法检测了Centrin同源基因在单环刺螠精子分化过程和早期胚胎发育过程的表达，结果显示：在单环刺螠精子发生过程中的精细胞、精子团、精子中都有表达，而在成体的体壁组织、消化道组织、体腔球中则没有表达；在未受精卵中没有表达，而在受精后释放极体的受精卵和经A23187孤雌激活的卵中则有表达；在单环刺螠的早期发育过程——二细胞胚胎、多细胞胚胎、原肠胚、早期担轮幼虫——中都有表达。

The results show that 154 species were found in the area,including 60 species of Polychaeta,30 of Crustacea,28 of Mollusca and 24 of Echinodermata;the dominant species are not very distinct,the relative important or common species are Listriolobus sp.,Xenophthalmus pinnotheroides White and Cossurella dimorpha Hartman.

本次调查共获得长江口附近海域大型底栖动物154种，其中多毛类环节动物60种，甲壳动物30种，软体动物28种，棘皮动物25种，其它类群动物11种。群落中优势种的地位都不明显，只有螠虫(Listriolobus sp。)、豆形短眼蟹(Xenophthalmus pinnotheroides White)、拟单指虫为相对重要的种类。

Futhermore, using indirect immunofluorescence to determine the centrin localization in trochophore cell and we found that many cluster centrin spots locate at cytoplasm.

我们用间接免疫荧光还检测了单环刺螠担轮幼虫分散的单细胞的Centrin表达，结果显示，除在中心体中有定位外，在纤毛基体中有Centrin定位。

We think that centrin play an important role in meiosis during Urechis unicinctus sperm metamorphosis difference and we also think that centrin may be related to trochophore cilia and sperm tail formation,and reconstraction of centrosome after fertilization.

我们推测Centrin在单环刺螠精子发生过程中减数分裂或精子细胞变态分化过程中发挥作用；在单环刺螠早期胚胎发育中，Centrin可能与受精后中心体的重建及担轮幼虫的纤毛形成有关。

A23187 and OA could parthenogenetically activate Urechis unicinctus oocytes and activate MAPK, indicating that the effect of fertilization on GVBD and activation of MAPK was mediated by rising in intracellular free calcium, and phosphorylation of some proteins was enough to activate the oocytes.

钙离子载体A23187和OA可以孤雌活化单环刺螠卵母细胞并激活MAPK。说明：①受精是通过钙离子的内流来激活卵母细胞并使MAPK活化的。②某些蛋白质的磷酸化状态对减数分裂是必要而且在一定程度上是充分的。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。