融合的

Interaction between fusion peptide of influenza virus A and its related antisense peptides was studied. A peptide was screened out with high binding ability to fusion peptide and the dissociation constant was shown at the level of umol/L. In order to quantitatively characterizing the affinity interaction, a system consisted of quartz crystal microbalance biosensor and flow injection analysis was designed and established.

据此初步结果，研究了甲型流感病毒融合肽与其反义肽的相互作用；提出了基于反义肽的组合化学法筛选目的多肽或蛋白质的亲和配基的全新的研究思路，筛选到了与融合肽具有强相互作用的多肽，其与融合肽的解离常数达到umol/L。

Taking the scientific verified method, argumentation explicative method, Marxism materialist dialectics and intuitive method of experience as the main frame, beginning with the stereoscopic vision of fusion and creation, this paper makes a macroscopic and systematic research on the academic methodology during Minguo period according to the internal line of reasoning of society change, academic debug and method creation.

本文尝试以科学实证方法、义理阐释方法、马克思主义唯物辩证方法、直觉体悟方法为主体框架，从融合与创新的立体视眼切入，按照社会转型、学术调适、方法创新的内在理路，对民国时期的学术方法论作一宏观、系统的研究，以此来凸现民国时期学术方法论的整体面貌和价值，并从历史和逻辑的层面揭示人类文化在不断接触中融合、在不断融合中创新、在不断创新中发展的客观规律。

The diachronical change of language and culture is the main reason of this historicity in translation classics.

蕴涵在典籍翻译中的两次视域融合分别是源文本的历时视域与译者的现在视域的融合以及译文的历史视域与译文读者的现在视域的融合。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

At last, ILIF in segment L3-4 and L4-5 is feasible. 2. There are enough operation space for ILIF in L3-4 and L4-5 with the root being retracted gently. And it is feasible to insert a couple transpedicle screws homolaterally. The intertransverse area was too narrow to perform ILIF on L5-S1.3. ILIF provide greater biomechanical stability than TLIF. while there was no significantly different stability between ILIF with homolateral or bilateral pedicle screw fixation. Adequate postoperative stability can be achieved by intertransverse lumbar interbody fusion with homolateral pedicle screw fixation.4. ILIF with transpedicle screws fixation homolaterally was a safe, convenient and effective operation, and less intraspinal complications were occurred of ILIF than TLIF.5. The fusion condition was satisfaction in ILIF group, which was better than TLIF group.

横突间入路腰椎椎体间融合术在L3-4、L4-5节段具有可行性。2、在L3-4、L4-5节段可提供足够的空间行ILIF手术，并可通过同一切口附加椎弓根钉固定，操作安全、简便。L5-S1节段横突间区域较小，操作困难，不适合行ILIF手术。3、ILIF手术术后生物力学稳定性有所下降但优于TLIF手术；ILIF附加同侧椎弓根钉固定与附加双侧椎弓根钉固定生物力学稳定性相当，使用ILIF术式附加侧同椎弓根螺钉固定，可提供较好的即刻稳定性。4、ILIF手术及附加同侧椎弓根钉固定是一种安全、简便、有效的手术方式，在减少椎管内并发症方面具有优势。5、ILIF手术可获得较好的融合率，其融合情况从组织学观察稍好于TLIF手术。

For the multisensor systems with uncorrelcted noises,and with unkonw noise varaies.substituting the information fusion noise variance setimators based on the correlct method into the centralized fusion optimal information filter,a self-turning centralized fusion information filter is presenced compared with the self-turning centralized fusion Kalman filter based on the Riccati equation,it can be avoid to compute zhe inverse matrix with high dimension,so that the calculation hurden can be reduced significantly.

对于带未知噪声系统和不相关噪声的多传感器随机系统，将基于相关方法得到的噪声方差带入到集中式融合最有信息滤波器，提出自校正集中式融合信息滤波器。同基于Riccatia方程的集中式融合Kalman滤波器相比它避免了计算高维矩阵的逆，从而减少了计算负担。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

According to the deficiency of Image Fusion applications in entertainment equipments, such as no special research that combines Virtual Reality, Self-control Photographing, Computer Vision and Image Fusion technologies together, we propose an Image Fusion algorithm base on Character of Apperceiving Contrast, which take system elements into account, e.g. integrity image quality balance and costs.

鉴于目前对图像融合技术在娱乐领域的应用缺乏研究，尤其缺乏将虚拟现实技术、自助照相技术、机器视觉技术和图像融合技术综合运用的分析研究，因此我们从分析人类视觉特征入手，运用系统的观念综合平衡图像质量、系统成本等技术指标，在对虚拟实时自助照相系统具体技术指标深入分析的基础上，提出了基于对比度感知特征（Character of Apperceiving Contrast）的图像融合算法。

By the modem time series analysis method, based on the ARMA innovation model, under the linear minimum variance optimal information fusion criterion, three distributed fusion steady-state optimal Kalman filters, predictors and smoothers weighted by matrices, scalars, and diagonal matrices are presented for multisensor systems with correlated input and observation noises, and with correlated observation noises. The Lyapunov equations and formulas of computing local filtering, predicting and smoothing error variances and covariances are given, which are applied to compute optimal weights. The corresponding three distributed fusion Wiener state estimators are also presented.

应用现代时间序列分析方法，基于自回归滑动平均新息模型，在线性最小方差最优信息融合准则下，对于带相关输入噪声和观测噪声和带相关的观测噪声的多传感器系统，提出了按矩阵加权、按标量加权和按对角阵加权的三种分布式融合稳态Kalman滤波器、预报器和平滑器，其中提出了局部滤波、预报和平滑估值误差方差阵和协方差阵的Lyapunov方程和计算公式，它们被用于计算最优加权，也提出了相应的三种分布式融合Wiener状念估值器。

We found that neither the fusioned nor the non-fusioned forms of Hu-P68 cDNA open reading frame could express efficiently in the E. coli cells; in different prokaryotic expression vectors and under various promotors resulted in no distinctive variations. Deletion of 5'UTR by synthesis adaptors, deletion of 3'UTR or signal peptide sequence by PCR gained no gross improvement. Only the truncated API-I proteins were observed to be highly expressed when the 3'ORF fragment of the Hu-P68 cDNA was fusioned at the 3'end of PAI-I gene.

结果发现无论是以融合形式存在，还是以非融合形式存在，人的P68 cDNA基因在E.coli细胞中都未能获得高效表达：在不同表达载体中，不同启动子对P68 cDNA基因的表达没有显著差异；利用合成Adapter的方法删除5'UTR，及利用PCR技术删除3'UTR和信号肽序列对表达没有明显改善；分别表达被分割的P68 cDNA5'端编码区、中央编码区和3'端编码区均未见有明显表达；当P68 3'端编码区与高效表达蛋白PAI-Ⅰ基因3'末端融合时，仅见有PAI-Ⅰ以截断形式高效表达出来。

I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时，滚道或滚动元件褪色是很常见的特征。