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The aim of this study was to examine caveolin-1 expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualizarjon and hormonal treatment by in situ hybridization and immunohistochemistry.

本实验以小鼠为材料,利用原位杂交和免疫组化方法,首次检测了小窝蛋白-1基因在小鼠早期妊娠子宫中的表达,并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究小窝蛋白-1基因在子宫中的表达与调节。

The aim of this study was to examine cPLA2α gene expression and regulation in mouse uterus during early pregnancy, pseudopregnancy, delayed implantation, artificial decidualization and hormonal treatment by in situ hybridization and immunohistochemistry.cPLA2α immunostaining was detected in the luminal epithelium and glandular epithelium of mouse uterus from days 1 to 5 of pregnancy.

本实验以小鼠为材料,利用原位杂交和免疫组化方法,检测cPLA2α基因在小鼠早期妊娠子宫中的表达,并利用假孕、延迟着床、人工蜕膜化及激素处理等模型研究cPLA2α基因在子宫中的表达与调节。

The aim of this study was to investigate PGT, 15-PGDH and CBR1 expression in themouse uterus during early pregnancy and their regulation in related models of pseudopregnancy,delayed implantation, artificial decidualization, steroid hormone treatment by in situ hybridization,RT-PCR and immunohistochemistry.

本研究利用原位杂交、免疫组织化学和RT-PCR等方法,检测了PG转运载体PGT、代谢酶15-PGDH和CBR1在小鼠早期妊娠子宫中的表达,并利用假孕、延迟着床及激活、人工蜕膜化、性腺类固醇激素处理等模型,研究了它们在小鼠子宫中的表达与调节。

From the early study of the pathogenesis of pre-eclampsia people have found its association with immunological function . In 1986 Ellis et al[1] had found that the extravillous chorionic cytotrophoblast contacting with the maternal tissue expressed high levels of Human leukocyte antigen-G, a major histocompatibility tissue-specific antigen . Extravillous chorionic cytotrophoblast with high levels expression of HLA-G proteins can invade the maternal deciduas and the maternal spiral arteries by displacing the endothelial lining and destroying most of the musculoelastic tissue of these arteries .

早期研究开始,许多学者即发现其与免疫学有密切关系,至1986年Ellis等[1]发现人类白细胞抗原(human leukocyte antigen,HLA) G分子高度表达在绒毛外滋养层细胞( extravillous cytotrophoblast,EVCT)上,即浸润至子宫蜕膜层甚至达子宫浅肌层以及子宫螺旋动脉内皮层,与母血及子宫组织直接接触的滋养细胞,这种独特的组织分布提示HLA-G分子在母胎免疫耐受方面起重要作用。

Objective: To investigate the expression of TRAIL in mouse uterine endometrium during embryo implantation and the significance in the apoptosis of decidual cells.

目的:检测TRAIL在小鼠胚胎着床过程中子宫内膜的表达,探讨它在蜕膜细胞凋亡中的作用。

The decidual NK cells were collected and cell subset change of the two groups was analyzed by flow cytometry.

目的通过检测弓形虫感染后早孕蜕膜自然杀伤细胞亚群的变化,探讨其与弓形虫感染致不良妊娠结局的相关性。

Objective: To investigate messenger RNA expression of vascular endothelial growth factor and its receptors in tubal pregnant decidual tissue and to explore their effects on tubal pregnancy.

目的:检测血管内皮生长因子及其受体(KDR、Flt-1)在输卵管妊娠蜕膜组织的表达,探讨其在输卵管妊娠中的作用。

Objective To study the effect of progesterone and Insulin-like growth factor (IGF-Ⅰand IGF-Ⅱ) on proliferation in human decidual stromal cells of early pregnancy in vitro.

目的 探讨孕激素及胰岛素样生长因子对体外培养的人早孕蜕膜基质细胞增殖活性的影响。

RESULTS: The rates of TNFR1 expressed in decidual glandular epithelia cells, stromal cells and blood vessel endothelial cells of abortion group were 77.4%, 93.5% and 67.7% respectively, and in the control group were 96.7%, 70.0% and 23.3% respectively.

TNFR1在蜕膜间质及血管内皮细胞中表达水平的升高及其在腺体上皮细胞中表达水平的下降,可能与不明原因早期自然流产有关。

In the clinical research, the normal expression of PRmRNA in the deciduas are important in maintaining early pregnancy.

通过研究,发现蜕膜PR低表达与肾虚型早期自然流产的发生有着密切的关系。

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