蛋白酶

Results:(1) The optimum conditions for protease induction were: 26℃, initial pH 6.0 and inoculative quantity of fungal hyphae=1g/100ml, using chitin as the inducer;(2) Pr1 was purified and its molecular weight was determined as 33kDa.

结果：(1)以几丁质为唯一碳氮源，在26℃，初始pH值6.0，1%的菌丝接种量为最佳诱导条件；(2)分离得到的类枯草杆菌蛋白酶，并测定其分子量约为33kDa。

METHODS: Except normal control group, the remaining 5 groups of rats were exposed to tobacco smoke and received a single intratracheally instillation of porcine pancreatic elastase to induce emphysema models.

除正常对照组外，其余5组大鼠均采用烟熏加气管内一次性滴注猪胰弹性蛋白酶法建立肺气肿模型。

Methods HAb18Fab was prepared by papain digesting HAb18, and it was radioiodinated with iodine-131 using high efficiency iodination method. The experiment consisted of two parts: ten nud mice bearing human hepatocellular carcinoma xenograft were injected intravenously with 131Ⅰ-HAb18Fab 3 700kBq/mouse, and six patients with hepatocellular carcinoma were injected intravenously with 131Ⅰ-HAb18Fab 111-166.5 mBq/body.

以木瓜蛋白酶切割肝癌单克隆抗体HAb18制备HAb18 Fab，应用高效碘标记法将131Ⅰ标记于HAb18 Fab，再以131Ⅰ-HAb18 Fab偶合物进行荷肝癌裸鼠及肝癌病人体内放射免疫显像。

Pinl is a unique peptidyl-prolyl cis/trans isomerases and can specifically isomerize the phosphorylated Ser/Thr-Pro bonds in certain proteins, thereby modulating their functions.

肤基脯氨酞顺反异构酶Pinl，是由酵母双杂交筛选出的一种功能蛋白，主要调节有丝分裂相关蛋白酶，对细胞周期运行起着非常重要的调节作用赶'〕。

The application of such solvent effects to the kinetically controlled peptide synthesis, however, has only recently been exploited.

试验结果表明，在所研究的几种单相共溶剂体系中，木瓜蛋白酶的酰胺酶活性都得到了不同程度的抑制，有些情况下甚至完全被抑制，而酯酶活性则保留了单水相中的全部或部分。

The results showed that Lactarius camphorates, which could inhibit HIV-1 PR by 80.9%, was the only sample exhibited over 80% inhibition on PR at 0.5mg/ml.

在已测的22种食用菌多糖复合物对HIV-1 PR的抑制活性实验中，反应浓度为0.5mg／ml下对蛋白酶抑制活性最高的是香乳菇，其抑制率达80.9％。

Nineteen spots were changed significantly after FA treatment.Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依靠磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Thirteen proteins were identified by peptide mass fingerprinting or peptide sequence analysis,including putative nucleoside diphosphate kinase,elongation factor 1-gamma,triosephosphate isomerase,60S acidic ribosomal protein P0,heat shock protein 75 kDa,similar to heat shock 70kD protein binding protein,annexin I,hypothetical protein FLJ34423,microtubule-actin crosslinking factor 1,lamin B2,ATP synthase alpha chain,mitochondrial precursor,proteasome subunit alpha type 6.These identified proteins involved in energy metabolism,translation and RNA processing,protein folding,redox regulation,cell structure and cell signaling.

双向凝胶电泳结果显示，甲醛刺激后19个蛋白斑点发生变化，肽指纹图谱及肽序列标签鉴定了其中13个蛋白斑点，已鉴定的蛋白包括二磷酸核苷酸激酶、延长因子1-γ，磷酸丙糖异构酶、60S酸性核糖体蛋白P0、75kDa热休克蛋白、70kD热休克蛋白样结合蛋白、钙依赖磷脂结合蛋白I、假想蛋白FLJ34423、微管-肌动蛋白交叉连接因子1、核纤层蛋白B2、ATP合成酶α链、蛋白酶体α亚基6，这些蛋白功能涉及转录调节、蛋白折叠、信号传导、能量代谢、细胞骨架等各个方面。

Leucocytic differentiation antigen 40 sign-al access participates in regulation of inflammatory reaction accommodation of major cell component (vascular endothelial cell、vascular smooth muscle cell and macrophage), it is playing critical role in AS process, participates ather-osclerotic occurrence and development ,reside in atherosclerotic plaque cell thro-ugh CD40-CD40L interaction, induce activate by itself , express and secrete profit to happen immune response、inflammatory reaction、hemagglutination t-hrombogenic proteo-cytokine.

白细胞分化抗原40 (CD40)信号通路参与了动脉粥样硬化斑块内主要细胞成分(血管内皮细胞、血管平滑肌细胞以及巨噬细胞)炎症反应的调节，其在AS进程中扮演着关键性的角色，参与了动脉粥样硬化的发生和发展；存在于AS斑块内的细胞通过CD40-CD40L相互作用，导致自身活化，表达和分泌了有利于免疫应答发生、炎症反应、血凝和血栓形成的蛋白质细胞因子，如激活在AS斑块中的关键性细胞成分如黏附分子、炎症因子、基质金属蛋白酶等的产生，导致斑块的不稳定和破裂，而最终导致粥样硬化斑块病变的恶化。

The locational expression of MMP-9 of abdominal aortic aneurismal tissue of 20 patients was detected with immunohistochemical technique.

主要观察指标：基质金属蛋白酶9在3种不同动脉血管条件下的组织定位表达结果及表达水平。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。