蛋白酶

Objective To express the N-terminal fragment of human mast cell chymase in E.coli and prepare its polyclonal antibody from mouse.

目的：在大肠杆菌中表达人肥大细胞类糜蛋白酶N端片段，并制备其鼠源性多克隆抗体。

The selective inhibition of enzymatic activities of tryptase and chymase by natural and synthetic protease inhibitors added novel information in the drug development process in mast cell research area.

天然和非天然性类胰蛋白酶和类糜蛋白酶抑制剂所具有的抑制上述酶水解活性的作用为与此类抑制剂相关的药物开发提供了新的信息。

Concludes: Tryptase and/or chymase contents and subtypes of mast cell are related to intracellular F-actin ring in the tissue of human skin.

人皮肤组织中肥大细胞内类胰蛋白酶和/或类糜蛋白酶的含量及其肥大细胞亚型与丝状肌动蛋白环相关联。

Immunohistochemical stains were used to show the tryptase + mast cell and the chymase + mast cell distribution in order to observe the change of the mast cell in the model group, the control group and the treatment group.

取右下肺组织，用抗类胰蛋白酶单克隆抗体和抗类糜蛋白酶单克隆抗体免疫组织化学染色观察实验组与正常组之间肥大细胞数量的变化，HE染色观察肺组织一般病理变化。

The results suggested that the chymase could convert Ang I to Angin vivo,and the radioimmunoassay method could detect the activities of ACE and chymase simultaneously with high sensitivity and credibility.

表明糜酶转基因小鼠心脏中可表达糜酶且糜酶能催化Ang II 的生成，而蛋白酶抑制剂结合放免分析是一种快速、灵敏的检测ACE及糜酶样活性的方法。

Results: HE stain showed the obvious thicken of the artery, RV/ and mPAP elevated obviously (P.05) in model group in comparison with the control group, they were also elevated obviously (P.05) in the model group compared with the treatment group. The tryptase + mast cell and the chymase + mast cell obviously increased in the model group compared with the control group, also increased in the model group compared with the treatment group.

平均肺动脉压力模型组动物较对照组明显升高(P.05)，模型组较治疗组也明显升高(P.05)；右心室／模型组较对照组明显增加(P.05)，模型组较治疗组也明显增加(P.05)，肥大细胞总数（类胰蛋白酶阳性的肥大细胞）及类糜蛋白酶阳性的肥大细胞模型组较对照组明显增多(P.05)，肥大细胞在治疗组较模型组明显减少(P.05)。

To study the function of chymase,the protease inhibitors including Lisinopril,Aprotinin and EDTA,and radioimmunuoassy were used to measure the activities of angiotensin converting enzyme and chymase in the heart tissues of transgenic mice harboring the human heart chymase gene and the controls.

为研究糜酶的功能，本文利用蛋白酶抑制剂Lisinopril、Aprotinin及EDTA结合放射免疫分析的方法同时测定了糜酶转基因小鼠心脏组织中血管紧张素转换酶（angiotensin converting enzyme，ACE）及糜酶样活性。

Keywords: mast cells;tryptase;chymase;carboxypeptidase;osteoarthritis

中文关键词：类胰蛋白酶；类糜蛋白酶；羧基肽酶；骨关节病

To study the expression of matrix metalloproteinase-2 (MMP-2) in human ciliary muscle cells after administration of travoprost.

研究曲伏前列腺素作用下，基质金属蛋白酶－2(MMP-2)在人睫状肌细胞的表达。

Proteases may be used at various pH values, and they may be highly specific in their choice of cleavable peptide links or quite non-specific.

蛋白酶需要在特定的pH中发挥效力并且对于断裂肽键具有或不具有特异性。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。