蛋白酶

Analyzing the change of DH, PCL and active calcium, and selecting each of best method. The results:(1) Dealing with the bone under high pressure ①High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;②Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ①High content of calcium: neutral enzyme, 1500u/L, 6h;②High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明：A、高压①骨粉及骨汤蛋白质含量最高：压力为0.05MPa，高压2.0h，骨水比为1：2；②骨粉中氨态氮最低：压力0.13MPa，高压4.0h，骨水比为1：3；骨汤中氨态氮最低：0.10MPa，3.0h，骨水比1：4；B、酶解①游离钙含量最高：中性蛋白酶，酶浓度1500u/L，酶解6h；②水解度最高：胰蛋白酶，酶浓度1：100，酶解6h；③PCL值最低：胰蛋白酶，酶浓度1：125，酶解6h；PCL值最高：碱性蛋白酶，酶浓度1500u/L，酶解8h。

The results:(1) Dealing with the bone under high pressure ① High content of protein in sheep bone or bone soup:0.05MPa, 2h, 1:2;② Low amino acids in bone powder:0.13MPa, 4h, 1:3; Low amino acids in bone soup: 0.10MPa, 3h, 1:4;(2) Hydrolysis the bone soup with enzymes ① High content of calcium: neutral enzyme, 1500u/L, 6h;② High DH: pancreatic enzyme, 1:100, 6h;③ Low PCL: Pancreatic enzyme, 1:125, 6h; High PCL: alkalescency enzyme,

结果表明： A 、高压①骨粉及骨汤蛋白质含量最高：压力为0.05MPa ，高压2.0h ，骨水比为1：2；②骨粉中氨态氮最低：压力0.13MPa ，高压4.0h ，骨水比为1：3；骨汤中氨态氮最低：0.10MPa ，3.0h ，骨水比1：4； B 、酶解①游离钙含量最高：中性蛋白酶，酶浓度1500u/L ，酶解6h ；②水解度最高：胰蛋白酶，酶浓度1：100，酶解6h ；③ PCL 值最低：胰蛋白酶，酶浓度1：125，酶解6h ； PCL 值最高：碱性蛋白酶，酶浓度1500u/L ，酶解8h 。

The 20S catalytic core of the 26S proteasome has the shape of a barrel made of four rings which are composed of seven differentαsubunitsα_1-α_7 at two outer rings and seven differentβsubunitsβ_1-β_7 at two inner rings at which the catalytic sites locate. Three of theβsubunits contain proteolyses sites, which are sequestered in the hollow interior of the 20S particle. Substrates enter the 20S through a narrow channel formed by theβsubunits, whose N-termini, depending on their conformation, can either obstruct or allow substrate entry and thus function as a gate. This entry channel is narrow and only permits passage of unfolded, linearized polypeptides.

哺乳动物26S蛋白酶体是由一个20S催化颗粒(catalytic particle，CP)和两个19S调节颗粒(regulatory particle，RP)组成的ATP(adenosine-triphosphate，ATP)依赖性蛋白水解酶复合体。20S CP是26S蛋白酶体的催化核心，它是由4个圆环堆叠形成的桶状复合物，其中两侧外环每个环是由α_1-α_7亚基组成，两个内环每个环是由β_1-β_7亚基组成，4个环的中央形成一个狭窄的内腔。2个β内环形成了20S CP的催化中心空腔，其内壁为催化活性位点所在地；外侧α环中心的孔道是底物到达催化中心空腔的通道，一般被α亚基上的N末端所封闭，阻止胞内非目的靶蛋白进入20S CP内被降解破坏。

These include lactoferrin ; elastase ; and cathepsin G .

这些措施包括乳铁蛋白；弹性蛋白酶和组织蛋白酶G 。

Objective To study the expression of cathepsin B in gastric carcinoma and its relationship with the prognostic indexes (tumor histologic types,TNM stages) of gastric carcinoma.

目的研究胃癌组织中组织蛋白酶B的表达及探讨组织蛋白酶B表达与胃癌预后指标(肿瘤组织学类型，TNM分期)的关系。

The new model targeted on Cathepsin K was proved to be feasible and effective in the screening for antiosteoporosis drug.

初筛以抑制木瓜蛋白酶的活性为指标，经筛选从1000株真菌中得到9株具有较强抑制效应的真菌，复筛采用对纯化的组织蛋白酶K的特异性抑制效应为指标。

Subsequent experiments with over-expression and RNAi further established that chymotrypsin upregulation was a ...

其后，通过将糜蛋白酶基因过表达和RNA干涉，进一步证实糜蛋白酶基因上调是引起溴氰菊酯抗性的原因之一。

The pancreatic proterses trypsin,chymotrypsin and elastase are all derived from zymogen precursors by proteolytic activation.

胰腺的蛋白水解酶—胰蛋白酶、胰凝乳蛋白酶和弹性蛋白酶都从酶原前体衍生而来，即由蛋白水解而活化的。

The results showed that the optimal pH were 2.0 and 6.5~7.5 for gastric protease and amylase,10.5 and 6.5 for intestinal protease and amylase and 7.5 and 8.0 for intestinal trypsin and chymotrypsin,respectively.

结果表明：胃蛋白酶和淀粉酶的最适pH分别为2.0和6.5~7.5，肠蛋白酶和淀粉酶的最适pH分别为10.5和6.5，肠胰蛋白酶、糜蛋白酶的最适pH分别为8.5和8.0。

Highly active inhibitor of fibrinolysin and chymotrypsin.

溶纤维蛋白酶和胰凝乳蛋白酶的高活性抑制剂。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。