蛋白酶

The effect of heating and ultrasonic pretreatment on duck meat proteolysis were studied by assaying the degree of hydrolysis.

实验对鸭肉浆分别进行热处理和超声波预处理，通过测定鸭肉水解后的水解度，探讨热处理及超声波预处理对鸭肉蛋白酶法水解的影响。

This putative cotton Ghcysp protein showed from 67% to 82% identity to the other plants.

Ghcysp蛋白质与其它植物半胱氨酸蛋白酶的氨基酸同源性为67％--82％。

Pretreatment of the sea cucumber produced freeze-drying ready-to-eat food and the methods of enzyme treatment were also studied.

用复合蛋白酶处理盐渍海参，作为加工冻干即食海参的预处理样品，研究其酶处理最适工艺。

Objective To investigate the expression of RECK and MMP-9 in the progresssion of bladder transitional cell carcinoma.

目的 探讨RECK、基质金属蛋白酶－9(MMP-9)在膀胱移行细胞癌发生、发展中的作用。

The increased expression of MMP-9 may be an important factor in promoting the progress and recrudescence of chronic rhinosinusitis.

基质金属蛋白酶9的表达增高，可能是促进慢性鼻窦炎发展和复发的一个重要因素。

AIM:We have paid more attentions on matrix metalloproteinase that has great roles in bone embryonic development, reestablishment and pathological process.

目的： 基质金属蛋白酶在骨胚胎发育、改建及病理过程中所起的关键作用已引起人们的重视。

To produce the scientific evidence for developing and manufacturing new antitumor drugs.Methods: 1 The inhibitory effect on cell growth of Hela was measured by MTT assay in treated or untreated groups (3.125, 6.25, 12.5, 25, 50μg/ml TAM and control) for three different treatment times (24h, 48h and 72h).2 Apoptosis and cell cycle were measured by FCM in four experimental groups (0, 4, 16, 40μg/ml TAM) for 48h.3 Adopting Wright and Giemse's staining to observe the morphology of Hela cells which treated with 40μg/ml TAM.4 Using invasion experiment to detect the Hela cells'invasive abilities which treated with 40μg/ml TAM.5 The protein expressional levels of P-ERK, ERK, C-myc and Cyclin D1 in Hela cells untreated or treated with 4, 16, 40μg/ml TAM for 24h were measured by Western blot.6 Expression of anti-apoptotic gene bcl-2, apoptotic gene bax and MMP-9 in Hela cells of four experimental groups (0, 4, 16, 40μg/ml TAM for 24h), were observed by revers transcription PCR.7 The protein expression of P-ERK, ERK, Bcl-2 and Bax in Hela cells treated with 40μg/ml TAM for 24h observed by laser scanning microscopes.

1采用四甲基偶氮唑蓝法检测不同浓度北豆根总碱(3.125、6.25、12.5、25、50μg/ml)处理不同时间(24、48和72小时)对Hela细胞增殖反应的抑制作用。2采用流式细胞技术(flow cytometry，FCM)检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用48小时，对Hela细胞凋亡及周期变化的影响。3瑞氏-姬姆萨染色后显微镜观察北豆根总碱(0、40μg/ml)作用24小时后Hela细胞形态学变化。4采用Transwell小室法检测北豆根总碱(0、40μg/ml)作用24小时后对Hela细胞侵袭性的影响。5采用免疫印迹方法检测不同浓度北豆根总碱(0、4、16、40μg/ml)作用24小时后，Hela细胞中磷酸化ERK、ERK、C-myc、CyclinD1的表达变化。6采用逆转录-聚合酶链反应(revers transcription PCR，RT-PCR)半定量检测北豆根总碱(0、4、16、40μg/ml)作用24小时，Hela细胞抗凋亡基因bcl-2、促凋亡基因bax、基质金属蛋白酶-9(MMP-9)的表达变化。7应用激光共聚焦显微镜(laser scanning microscope，LSM)观察北豆根总碱(0、40μg/ml)作用24小时后，Hela细胞内P-ERK、ERK、Bcl-2、Bax蛋白的表达变化。

Different biological and ecological characteristics, virulence, sporulation, conidia bourgeon, Protea-Bse activity leve and colony growth of 6 strains of Beauveria bassiana were measured respectively. The results were calculated by grey relational analysis and the optimum sequence was built from the professional comparison of each strain's characteristics. Two strain's have a potential of appling in mass production, BD-B015 and BD-B038 were abtained. The revisory mortality to Laphygma exigua Hubner were 88.33% and 86.70%.The infected rate were 86.67% and 85.27%.

测定初筛获得的6个球孢白僵菌菌株的毒力、产孢量、萌发率、胞外蛋白酶活性及菌株生长速度等多个生物、生态学指标，从专业角度构建最优序列，经灰色分析和不同菌株毒力效价显著性比较，筛选出了两株具有较大生产潜力的优良菌株BD-B015和BD-B038，对甜菜夜蛾的校正死亡率分别为88.33%和86.70%，僵虫率分别为86.67%和85.27%。

Objective To obtain human rhinovirus 3C protease as a novel tool enzyme for gene engineering.

目的 获得重组3C蛋白酶，开发一种新型的基因工程工具酶。

Hilgenfeld said the researchers found close similarities between the protease enzyme of rhinovirus and the protein in SARS, which is a type of coronavirus.

Hilgenfeld说研究人员已经发现了鼻病毒的蛋白酶与SARS病毒的蛋白质间的一些相似之处。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。