蛋白酶

Several inducers cicada exuviae, colloidal chitin, horsefly cuticle, shrimp, maggot and silkworm chrysalis were compared with their effects on production of cuticle-degrading protease of entomopathogenic fungus Metarhizium anisopliae.

研究了不同碳氮源对金龟子绿僵菌产生与分解昆虫外壳相关的蛋白酶活性和同工酶谱带的影响。结果表明：蝉蜕、虻虫壳、蝇蛆壳、蚕蛹壳、虾壳、胶体几丁质均可使金龟子绿僵菌产生蛋白酶，虾壳和胶体几丁质所产生的蛋白酶比活性最高，蝉蜕次之。

Furfur was inoculated to the mycelial culture liquid medium and the rapeseed oil culture liquid medium, and then incubated in vibrator to induce the production of mycelial phase and yeast phase of M.

用全脂牛奶平板法、Folin-酚法、QuantiCleave蛋白酶试剂盒测定菌丝相及酵母相糠秕马拉色菌胞外蛋白酶的活力，并检测温度、pH值对蛋白酶活性的影响。

The fermentation of mucor protease in condition of liquid submerged culture of AS3.2778 and the characteristics of the protease were studied in this research.The Application of mucor protease in the novel ways of sufu fermentation were also studied in this paper.

本文主要研究了在液体培养条件下雅致放射毛霉产蛋白酶特征和蛋白酶的酶学性质，并对雅致放射毛霉蛋白酶在新型腐乳生产中的应用进行了探讨。

In order to find out the proteinase suitable for soybeen peptide preparation,studies were conducted on the properties of mucor producing protease,the preparation method of mucor protease,the decomposition of soybeen protein isolated by mucor protease,and the molecular weight of the enzymatic decomposition products.

为探寻适合于制备大豆多肽的蛋白酶，对毛霉产蛋白酶的特性、毛霉蛋白酶的制备方法及其对大豆分离蛋白的降解进行了研究，并测定了酶解产物的相对分子质量。

It shows us that the sufu can also treated as functional food. The biochemical process keeps unknown during sufu making. My study focuses on preparation, purification and hydrolysis properties of protease of Mucor piriformis Fischer which is a common used strain for process sufu. The main results are followed.

因此本论文选用用于腐乳生产的菌种梨形毛霉作为蛋白酶的生产菌种，从蛋白酶酶制剂的制备方法、性质的测定以及蛋白酶的分离纯化到该酶对大豆分离蛋白的降解作用逐一进行了研究，获得了以下主要结果。

The additional supplements including glucose,ascorbic acid and insulin had the enhancement effects on C.andersoni development,while folic acid had no significantly effect on C. andersoni development and calcium pantothenate had inhibited effect.When added 50 mmol/L glucose,50μg/ml ascorbic acid,0.3 U/ml insulin into 10%FCS RPMI-1640 medium,the proliferation of C.andersoni had a great significantly increase,and the gene copies of C.andersoni were 8-fold compared with that in 10%FCS RPMI-1640 medium.

冻融法+蛋白酶K法+声裂法提纯隐孢子虫卵囊DNA效果最佳，其次为冻融法+声裂法和蛋白酶K法+声裂法，冻融法+蛋白酶K法效果最差。10%FCS是培养安氏隐孢子虫的最佳FCS浓度，葡萄糖、维生素C和胰岛素都具有促安氏隐孢子虫增殖作用，泛酸钙无促虫体增殖效果，而叶酸则抑制虫体增殖。10%FCS RPMI-1640培养液中加入50 mmol/L葡萄糖，50μg/ml维生素C和0.3 U/ml胰岛素促安氏隐孢子虫增殖效果最显著，虫体增殖8倍。

Proteinase inhibitors play an important role in the research of enzyme studies and the structure function relationship of proteins.

蛋白酶抑制剂在酶学及蛋白质的结构与功能关系研究中有重要意义，Kunitz型丝氨酸蛋白酶抑制剂是其中最重要的，也是研究最广泛的蛋白酶抑制剂之一。

The effect of temperature, pH and proteinase inhibitor on this assay were also evaluated.

furfur正常皮肤分离株蛋白酶活性，并检测温度、pH值和蛋白酶抑制剂对蛋白酶活性的影响。

Objective:①To study the extracellular proteinase activity of M.

目的：①检测糠秕马拉色菌胞外蛋白酶活性；②研究菌丝相及酵母相蛋白酶活性的差异；③蛋白酶活性的影响因素。

Acidity protease, neutral pro-tease and basicity protease was used to obtain active biological peptides from fruiting body in Armillaria luteo-virens Sacc.

实验采用酸性蛋白酶、中性蛋白酶和碱性蛋白酶水解黄绿蜜环菌子实体蛋白制备生物活性肤。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。