蛋白酶

Reversible inhibitor of aspartic proteases, e.g. pepsin, cathepsin D, chymosin,rennin.

可逆的抑制天冬氨酸蛋白酶，如胃蛋白酶、组织蛋白酶D、凝乳酶

Abstract］ Objective To study the influence of different residual cornea bed of laser in situ keratomileusis on the collagenic construction of cornea through investigating the expression of matrix metalloproteinases and tissue inhibitors of metalloproteinases in cornea cells for different residual stroma bed after LASIK for six months.

目的 研究不同切削厚度的准分子激光原位角膜磨镶术（laser in situ keratomileusis，LASIK）后兔眼基质金属蛋白酶（matrix metalloproteinases，MMPs）及基质金属蛋白酶组织抑制剂(tissue inhibitors of metalloproteinases，TIMPs)的表达，以探讨LASIK手术的不同切削深度对角膜的胶原结构的影响。

The study showed the recombinant wt/mCREG protein depressed the VSMC proliferation depending on dose and the optimal concentration was 400nM;2biologic function of CREG protein and the membrance receptor mechanism:①effect on VSMC migration: the wound healing experiment showed the OB2 cells migration was slower significantly after added wt/mCREG(400Nm) in supernatant. The HITASY cells migration were very slowly and no remarkable change. The gelatinase digestion and Western blot analysis showed the matrix metalloproteinase was decreased and TIMPs was increased;②effect on differentiation: after added wt/mCREG(400nM), the expression of myocardin, SMα-actin, MHC and caldesmin were increased and that of LM-1 and FN were decreased in OB2 cells. These effects were more significant when adding wtCREG.;③effect on VSMC proliferation: Cell cycle assay and BrDU stain showed: after added the wtCREG and mCREG protein, the ratio of cell in G0/G1 phase increased to 0.5773 and 0.5572 from 0.5308 respectively in OB2 group, which increased to 0.7369 and 0.7034 respectively from 0.6297 in HITASY group;3Role of M6P/IGF2R in CREG biologic function:①ELISA and co-immunoprecipitation showed the wt/mCREG binding to M6P/IGF2R directly.②antibody blocking test: when the anti-IGF2R was added to medium at the same time with wt/mCREG at different concentration(2μg/mL、4μg/mL、8μg/mL),the effects of CREG protein which depressing proliferation, migration, secretion and promoting differentiation were blocked, which had the positive correlation to the concentration of added anti body. The studies showed two combinant CREG promoted VSMC switch to differentiation phaenotype, at the same time, depress VSMC proliferation, migration and secreting extracellular matrix.

上述实验结果证实：两种重组CREG蛋白对VSMC增殖均有剂量依赖性的抑制作用，并且相同浓度的糖基化的CREG蛋白对细胞增殖的抑制效应更为显著，最佳效应浓度为400nM；2两种重组CREG蛋白添加后对HITASY和OB2细胞生物学行为的影响：①CREG蛋白对VSMC迁移的影响：刮伤实验发现，加入最佳效应浓度的wtCREG和mCREG蛋白24h后，OB2组迁移能力下降，HITASY组无明显变化；细胞外基质金属蛋白酶-2，9(Matrix metallo-proteinase 2，9，MMP2 ，9)明胶酶电泳检测和Western blot检测结果证实，两种CREG蛋白均可以使OB2细胞合成细胞外基质MMP2，9减少，而组织金属蛋白酶抑制物(Tissue Inhibitors of Metalloproteinases，TIMPs)增加；②CREG蛋白对VSMC分化的影响：加入400nM的wtCREG和mCREG蛋白12h后，OB2细胞myocardin、SMα-actin、MHC、caldesmin表达增加，LM-1、FN表达减少；③流式细胞仪分析细胞周期和BrDU染色分析证实，加入400nM的wtCREG和mCREG蛋白后，OB2组G0/G1期细胞由0.5308分别增加至0.5773和0.5572，HITASY组G0/G1期细胞由0.6297分别增加至0.7369和0.7034；3M6P/IGF2R在重组CREG蛋白的生物学功能中的调控作用：①免疫共沉淀和免疫荧光双染色分析结果显示，CREG蛋白与M6P/IGF2R存在直接结合；②应用抗体阻断实验：将不同浓度的anti- M6P/IGF2R(2、4、8μg /mL)与两种CREG蛋白同时加入培养液中，CREG蛋白抑制VSMC增值、迁移和合成细胞外基质、促进分化的效应减弱，而且与加入anti- M6P/IGF2R浓度正相关。

Response surface analysis(central composite design of uniform precision) was applied to optimize the significant four factors[the ratio of bran and soybean meal,(NH4)2SO4, NaNO3 and KH2PO3] for culture medium of Aspergillus terreus by researching the contributiveness of factors and their interactions to the enzyme activity of acidic proteinase.

用响应面分析法（采用中心组合一致精度的设计）对影响土曲霉产酸性蛋白酶固态发酵培养基的4个主要影响因素（麸皮与豆粕比例、硫酸铵、硝酸钠、磷酸二氢钾）进行了优化，考察了各因素及其交互作用对酸性蛋白酶酶活的影响。

Then histochemical, electron microscopic, enzyme histo cytochemical and confocal laser scanning microscopic methods were employed to study the distribution of vinculin, cathepsin Dand nematolysosomes ...

结果在正常对照组神经元，组织蛋白酶D与纽蛋白分布于胞质及突起内；在CD及PMA处理神经元，纽蛋白及组织蛋白酶D的分布呈向心性移动，但集聚的部位不同；电镜酶细胞化学方法显示CD组及PMA组神经元内线状溶酶体均增多。

Since 1962, when Gross and Lapiere discovered the first MMPs-collagenase, man had discovered various of MMPs in succession, where MMP-2 has a strong degradational effect on extracellular matrix, it is secreted bymacrophage, smooth muscle cells and endothelial cells and so on.

自从 1962年 Gross和 Lapiere发现第一种基质金属蛋白酶一胶原酶以来，人类陆续发现了很多种基质金属蛋白酶，其中的MMP—2对细胞外基质具有极强的降解作用，它由巨噬细胞、平滑肌细胞以及内皮细胞等分泌，在动脉粥样硬化斑块中憎多时降解纤维帽及基底膜的胶原等基质成分，是使纤维帽变薄及加速动脉粥样硬化的重要因素之一。

Objective To study the relationship of endangium proliferation and the expression of metalloproteinases and tissue inhibitors of metalloproteinases after angioplasty of iliac artery in rabbits to investigate the probable mechanism of composite Danshen pill in prevention and treatment of restenosisMethods 30 white male rabbits of Japan （the average body weight was 25～30 kg）were randomly divided into 3 groups:normal control group,model group (intima destroyed by balloon and given hypercholesterol diet),CDP group (intima destroyed by balloon and given hypercholesterol diet plus drug CDP 150 mg/d),10 ones each groupResults The results of iliac artery angiogram and the analysis of pathology:there were lumens stenosis,thinner intima,smaller intima area,in CDP group compared with those in model group,and ratio of intima and tunica media thickness and area among each group (P＜001)Immunohistochemistry analysis:the MMP2,MMP9,TIMP1,TIMP2 in model group significantly increased than those in control group (P＜001)The CDP group had lower MMP2 and MMP9 expression,higher TIMP1 and TIMP2 expression,and increased MMP2/TIMP1,MMP9/TIMP2 than model group (P＜001)The thickening of vascular neointima and stenosis degree of vascular lumen were relevant to MMP2 and MMP9 (r=0896,P＜001)Conclusions MMP and TIMP play the very important role in the restenosis process of arteryCDP could inhibit the thickness of vascular neointima after balloon injury,the probable mechanism of which may be inhibiting collagen formation,smooth muscle immigration and decreasing hyperplasia of intima by interfering expression of MMPs and TIMPs

目的 研究兔髂动脉成形术后血管内膜增生和基质金属蛋白酶及其抑制物表达之间的关系，探讨复方丹参滴丸预防再狭窄的可能机制。方法健康成年二级雄性日本大耳白兔30只，平均体重25～30 kg。随机分为3组：正常对照组10只，模型组10只（球囊内膜剥脱加高胆固醇饮食），治疗组10只（球囊内膜剥脱加高胆固醇饮食以及复方丹参滴丸150 mg/d）。结果兔髂动脉造影、血管病理图像分析检测结果：①复方丹参滴丸组较模型组血管造影示管腔直径狭窄、内膜厚度减少、内膜面积减少、内膜厚度和中膜厚度比、面积比，各组之间有显著性差异(P＜001)。②免疫组化分析：模型组MMP2、MMP9、TIMP1、TIMP2表达均高于对照组（P＜001）；复方丹参滴丸组MMP2、MMP9的表达低于模型组，而TIMP1、TIMP2的表达高于模型组；TIMP1/MMP2、TIMP2/MMP9明显增加，差异有显著性（P＜001）。③内膜的增生以及管腔的狭窄程度与MMP2、MMP9有很好的相关性(r=0896，P＜001)。结论 MMP和TIMP在再狭窄形成中起重要作用；复方丹参滴丸能够明显抑制血管损伤后的内膜增生，可能的机制是通过影响金属蛋白酶MMP2、MMP9及其抑制物TIMP1、TIMP2的表达从而抑制胶原的生成、平滑肌的迁移、增生，而减少内膜的增生。

This sequence compare to the sequence of PI from ShanXi ShouYang buckwheat once be reported by Zhang Zheng and others,the homology is 64%, From this we can conclude that this gene is a new member of PI from Fagopyrum.

该序列与张政等人报道的山西寿阳甜荞蛋白酶抑制剂基因同源性为64％，由此表明该基因可能为荞麦属蛋白酶抑制剂家族的一个新成员。

The experimental results revealed that papain, calcium chloride, compound phosphate, and glucide keeping humidity reagents intensify the tenderness of pork jerky, but the addition should be proper. The best proportion was papain0.4‰, compound phosphate 0.4%, calcium chloride 0.1%, and sorbitol 1.5%.

实验结果表明：木瓜蛋白酶、复合多聚磷酸盐、氯化钙和糖类保湿剂都可增加肉脯的嫩度，但用量应适当，添加量过大，反而使肉脯的感官品质变差；肉脯嫩化的最佳配比为木瓜蛋白酶0.4‰、复合多聚磷酸盐0.4%、氯化钙0.1%、山梨糖醇1.5%。

Objective: To investigate the effects of Hovenia dulcis extract on mRNA expression of MMP-13 and TIMP-1 mRNA in hepatic tissue in experimental rats.

中文摘要：目的：探讨枳子提取物对实验大鼠肝组织基质金属蛋白酶-13(MMP-13)与金属蛋白酶组织抑制因子-1(TIMP-1)表达的影响。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。