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蛋白质晶体

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ABSTRACT A number of protein functions are under exploration based on the determination of their 3D structures.

蛋白质结构分析的经典方法是x射线晶体学和多维核磁共振技术。

A simple protein found in horny and cartilaginous tissues and in the lens of the eye.

角质或软骨组织以及眼球晶体中的简单蛋白质。

Appling proteomics,we can detect various of characteristic proteome,which induce intramolecular or extramolecular aggregation in α-crystallin and reduce its activity and accelerate the cataract formation.

白内障发生时,多种疾病特征性的蛋白质组改变,可导致α-晶体蛋白分子内或分子间的交联,分子伴侣活性下降,加速白内障的形成。

The active site structure of the molybdenum oxotransferase is likely to change during foster the molybdenumenzyme crystal because of the sensitivity and friability of the protein, which can make the result warp.

由于蛋白质极其敏感和脆弱,在进行酶处理培养晶体的过程中可能引起钼酶活性因子的结构变化,从而使测定结果出现偏差。

Humans orientate in 3D by using otoliths, small crystals of calcium carbonate and protein that shift on hairs in the inner ear. Forces acting on these grains as a person moves mean they can sense acceleration and gravitational pull.

人类通过利用耳石、小晶体碳酸盐和内耳毛发中的蛋白质来产生三维立体感,当一个人运动时,力量就会施加在这些微粒上,从而让人感受到加速度和重力牵引。

Using high performance liquid chromatography, X-ray diffraction and Fourier transformation infrared spectra, the authors characterized the sorted proteins from different otoliths. It is found that the three types of proteins in lapillus and asteriscus have different characteristics, and such differences may be responsible for different kinds of crystalline state in calcium carbonate.

通过高效液相色谱、X射线衍射、红外光谱分析和氨基酸成分分析等方法分别对3种基质蛋白进行了表征,发现微耳石和星耳石中3种基质蛋白特征存在差异,并初步推断蛋白质特征的差别与碳酸钙晶体在微耳石和星耳石中的不同晶型有关。

The adsorption of bovine serum albumin, an ellipsoidal model protein, on the surface of the multilayers was studied in detail by means of a quartz crystal microbalance with dissipation monitoring.

应用具有耗散因子功能的石英晶体微天平检测了多糖多层膜的构筑过程以及蛋白质在多层膜上的吸附行为。

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制,酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力,酶蛋白亚基间的相互作用,酶蛋白的折叠以及内运动状态与酶活力的关系,酶蛋白发挥功能时的结构特性;运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性,热稳定性机制;确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位;确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构;确定了一个新型的染色质蛋白Cren7的溶液三维结构;正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

Using the cDNAs from primary spermatocytes and round spermatids, the cDNA subtractive libraries of these cells were constructed by the application of the technology of suppression subtractive hybridization.

我们得到了这个蛋白质的晶体并且对它进行了初步的晶体学分析。人类FcaRI(CD89)分子是黏膜和血清中含量极其丰富的IgA抗体的特异性受体。

For example, they may learn that Protein X and Condition A resulted in a micro crystal, and Protein A and Condition Z resulted in a micro crystal as well.

举例来说,他们发现蛋白质X和条件A导致了微晶体,蛋白质A和条件Z也导致了微晶体。

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Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

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