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In the prediction of protein structural classes, the residue order along the sequences takes an important role; while as in the prediction of protein subcellular locations, the amino acid composition is the most important factor.

在蛋白质的亚细胞定位预测中,氨基酸组成仍然是最重要的特征,蛋白质的亚细胞位置与序列同源性的关系没有结构与序列同源性的关系强。

Common strategies and examples of protein engineering in improving protein solubility, homogeneity, and crystallizability are presented.

近年来分子生物学尤其是蛋白质工程的应用有效地提高蛋白质的溶解度、均一性及可结晶性等内在特性,促进蛋白质的结晶,成为提高蛋白质结晶能力和蛋白质晶体分辨率的有效途径。

In the second part, I studied the effects of protein complexity (here defined as the number of subunit types in a protein) on gene dispensability and gene duplicability using functional genomic and protein structural data.

接下来,我利用功能性基因体及蛋白质结构资料,探讨蛋白质复杂性(蛋白质次单元种类的数目,protein complexity)对基因的可移除性及可复制性的影响。

Except for variation data, gene descriptions, enzyme information and other biological information for each gene locus are also included. Since RefSeq of NCBI http:∥www:. ncbi. nlm. nih. gov/LocusLink/refseq.

另外,VSD有别于现有的其他变异数据库的主要特点是:一是在基因组、mRNAs和蛋白质三个水平上定位了变异;二是分析了可能影响蛋白质功能的非同义单核苷酸多态性变异(nonsynonymous SNP,nsSNPs)。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合,而且结合是饱和性的、可被竞争的,符合配体-受体结合的典型特征,同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性,表明水稻细胞质膜上存在nsLTP的受体;利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜,再进行结合实验,结合活性几乎不受影响;而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失,表明其受体为没有经过糖基化修饰的蛋白质;利用交联剂BS~3交联配体一受体后,再进行SDS-PAGE分离和放射自显影,结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合,可能是其受体;细胞外碱化实验表明,nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应,却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应,表明nsLTP和Cryptogein结合细胞膜上相同的位点,保护了植物细胞免受Cryptogein导致的细胞程序性死亡,并诱导系统获得性抗性的产生;体外Pull-down实验表明,nsLTP144和水稻的OsCaM1具有相互作用,暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

The TVB-N and total bacterial counts of razor clam with the addition of complex biological preservatives consisting of lysozyme and / or nisin increased more slowly during storage than ; hat.

盐溶性蛋白质溶解度的变化与蛋白质变性密切相关,可能也与缢蛏的鲜度存在较大关系。

In total, 22 differential proteins were identified from 9 fractions.

研究结果表明,利用基于蛋白质水平的在线二维液相色谱分离技术找寻差异蛋白质,具有重现性好、自动化程度高等优点,可用于开展比较蛋白质组学的研究。

In this paper, the study status of enhancing the protein emulsification by modification methods such as deamidation, proteolysis, glycosylation, phosphorylation and graft copolymerization were introduced, the development direction of the preparation of new type protein emulsifier was also pointed out,it provided a reference for the future study.

综述了脱酰胺作用、蛋白水解作用、糖基化作用、磷酸化作用、接枝改性等蛋白质改性技术对提升蛋白质乳化性的研究现状,展望了制备新型蛋白质乳化剂的发展方向,为今后蛋白质乳化剂的研究提供参考。

I found that the major distinction for gene duplicability in protein complexity is between hetero-complexes, each of which includes at least two different types of subunits, and homo-complexes, which include monomers and complexes that consist of only subunits of one polypeptide type.

接下来,我利用功能性基因体及蛋白质结构资料,探讨蛋白质复杂性(蛋白质次单元种类的数目,protein complexity)对基因的可移除性及可复制性的影响。

In order to investigate the mechanism of urea gradient enhancing protein refolding, the renaturation kinetics of the two proteins was studied.

为了研究尿素浓度梯度促进蛋白质复性的机理,对两种目标蛋白质的复性动力学方程进行了研究。

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On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面,更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值,分为几个相等的区间作为横轴,并将各区间内所测定值依所出现的次数累积而成的面积,用柱子排起来的图形,也叫做柱状图。

You rap, you know we are not so good at rapping, huh?

你唱吧,你也知道我们并不那么擅长说唱,对吧?