蛋白质

This proposed method has a potential advantage to design other protein probe with linear aptamer with a complex structure and can be used as a simple and general tool for protein detection.

采用该方法检测凝血酶的线性范围为0.5~8 nmol/L，检测下限为0.5 nmol/L，为蛋白质检测提供了一种简便快速的非直接标记的荧光分析方法，有望在蛋白质组学的研究中得到广泛的应用。

Studies on longans specific proteins in the embryo development stagesIEF-SDS-PAGE technique was applied to comparing and analyzing the changes of protein component during longan embryos differentiation and development.

福建农林大学硕士学位论文 3龙眼胚胎发育时期的特异性蛋白质应用双向电泳技术分析研究了龙眼胚胎发育时期蛋白质组分的变化。

The results presented in this paper indicated that sodium salt of 3,7-bishemis uccinate of 7-hydroxycholesterol (RS 634) at the dose of 45mg/kg/day (×7,ip) could increase DNA, RNA and protein syntheses in bone marrow cells and protein synthesis in liver,spleen and serum, but no markedly effects on DNA and RNA synthesis in liver and spleen of mice bearing EAC were observed, In the subacute test on rats RS 034 (400mg/kg/day, x30 ip) showed no evident effects on the number of RBC, WBC and the percentage of lym...

本文报告的结果表明7β-羟基胆固醇双琥珀酸单酯钠(RS 024)在有效抗肿瘤剂量下(45mg/kg/day，×7ip)对荷瘤小鼠骨髓细胞DNA、RNA和蛋白质合成有促进作用，对肝、脾的DNA、RNA合成无显著影响，但能促进肝，脾中及血清蛋白质合成。按400mg/kg/day连续给药30天，对大鼠红细胞、白细胞数及淋巴细胞百分率无明显影响。提示该药在有效抗癌剂量下毒性较小，并在一定程度上对免疫功能有增强作用。

Proteins localized to the inside of the lysosome, for example, often have roles in protein degradation.

例如，位于溶酶体中的蛋白质通常具有降解蛋白质的功能。

There are differences in the expression of Adrenal glands proteins in the hyperthyroidism rats with hyperactivity of liver-YANG after treatment with PGQY, and the cure mechanism of PGQY may associate with antioxygen, accommodation the functions of peripheral sympathetic nerve and adrenal medullae ,regulation of enzyme concerned with energy metabolism through these 10 identified protein spots.

平肝潜阳药物治疗后的甲亢肝阳上亢证大鼠肾上腺蛋白质的表达上具有差异，平肝潜阳药物的作用机制可能与所鉴定的这10个蛋白质的抗氧化、调节交感－肾上腺髓质功能及影响能量代谢相关酶等作用有关。

Among the present methods for protein detection using aptamers, some of them are with high sensitivity, some with high sensitivity but require expensive instrument. This study takes use of aptamer modified by Au naloparticle to construct a new method for protein detection. This methos can fastly detect proteins with high sensitivity compairing to other methods, and requires no expensive instruments. It has wide application prospect and large market value.

当前利用Aptamer的蛋白质检测方法，有的灵敏度不是很高，有的虽有较高灵敏度，但需要昂贵的仪器，本研究利用金纳米颗粒(Au nanoparticle，Au NP)修饰的Aptamer来建立一个蛋白质检测的新方法，与其他方法相比，本方法有较高灵敏度、不需昂贵仪器，简单快捷，具有广泛的应用前景和巨大的市场价值。

The binding reaction of methyl blue with proteins enhances remarkably theresonance Rayleigh light-scattering signal at maximum wavelength of 350 nm and forms complexes.

在pH2.36的BR介质中，甲基蓝与蛋白质相互作用生成复合物，使最大波长350nm的共振光散射光谱得到增强，增强程度与蛋白质浓度成正比。

In the second chapter , we studied the resonance light scattering spectral characteristic of tichromine-protein system, and the elementary reaction mechanism with absorption spectra method and a lot of condition experiments. Another new determination method of protein at nanogram level was established.

第二章研究了钛色敏-蛋白质体系的共振散射光谱特征，并结合吸收光谱法和一系列条件实验初步探讨了它们的作用机理，建立了一种测定纳克级蛋白质的新方法。

A uricase gene was cloned and sequenced from Bacillus subtilis strain CCRC 14199. The cloned uricase gene contained an open reading frame of 1491 nucleotides encoding a protein of about 55 kDa. A uricase-MBP fusion protein of about 98 kDa was expressed and purified.

尿酸氧化酵素基因是来自枯草杆菌CCRC14199的重组DNA，其基因序列包含1491 核甘酸约55 kDa蛋白质，及表现在麦芽糖结合蛋白大约98 kDa之融合蛋白质，并呈现高尿酸氧化酵素的活性(9.1 U/mg)。

In the protein of barley, the contents of Glu, Pro, Phe, Arg are high, and the Limitation amino acids are Met and Trp. The content of Lys in barley is higher than that in wheat, which showed it had a good nutrition quality. The reason for barley protein unforms gluten net tissue was the content less of prolamine and glutein in the protein of barley. The one of main factors for barley showing high biological activity functions might lie in its high β-glucan and polyphenol chemical compound content.

大麦蛋白质中谷氨酸、脯氨酸、苯丙氨酸和精氨酸含量较高，限制性氨基酸为蛋氨酸和色氨酸，赖氨酸的含量高于小麦的，表现出较好的营养品质；大麦蛋白质组分中醇溶蛋白和麦谷蛋白含量较低，是其不能形成面筋网络组织的主要原因；大麦中含有β-葡聚糖和多酚类化合物是其生理活性功能较强的主要因素之一。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。