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蛋白质

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Methods Proteins and peptides in CSF were separated by two-dimensional gel electrophoresis(2DE) and ultra-performance liquid chromatographer and identified by matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF-MS) and electrospray ionization to study differential proteomic expression in the cerebrospinal fluid between patients with Demy- elinative disease and with other neurological diseases.

方法以中枢神经系统脱髓鞘疾病患者的脑脊液为研究对象,分别采用固相pH梯度双向凝胶电泳分离总蛋白质,凝胶经银染显色后,用图像分析软件进行比较分析、识别差异表达的蛋白质,离线应用基质辅助激光解析电离飞行时间质谱得到相应的肽指纹图谱;同时用超高压液相色谱分离酶解肽段,在线采用电喷雾四极杆飞行时间质谱鉴定肽段氨基酸序列;搜索数据库鉴定部分蛋白质

And then the two fragments were spliced in vitro at the temperature of 16℃,25℃,37℃.The reaction can promote the formation of terminal dimerization so that we can reason out the answer to the specific binding between IN and IC.

通过本论文的研究,在蛋白质内含子高级结构未知的情况下,筛选获得高剪接活性的蛋白质内含子,完成了对蛋白质内含子功能的进化。

OBJECTIVE: To introduce proteomics, to summarize the research of proteomics in directional differentiation of BMSCs, and to forecast the development of proteomics research methods.

目的:介绍蛋白质组学的概况,总结蛋白质组学在骨髓间充质干细胞向心肌、成骨及脂肪细胞定向分化方面的研究进展,讨论其面临的问题,并对蛋白质组学研究方法进行展望。

In the second part, I studied the effects of protein complexity (here defined as the number of subunit types in a protein) on gene dispensability and gene duplicability using functional genomic and protein structural data.

接下来,我利用功能性基因体及蛋白质结构资料,探讨蛋白质复杂性(蛋白质次单元种类的数目,protein complexity)对基因的可移除性及可复制性的影响。

Also in the 3-D space, we assign the 20 amino acids to 20 vertices of the dodecahedron. By the symmetry of the dodecahedron we obtain 3-D representation of 20 amino acids, and 3-D graphical representation and the corresponding numerical sequence of protein sequences. And similarity and dissimilarity analysis based on the invariants of graphs and characteristics of numerical sequences are given for nine RNA secondary structures of RNA-3 of virus. We construct sequence phylogenetic tree of a group of cytochromes C protein.

在DNA三联体密码子表示的基础上,在半复平面上给出了蛋白质序列的非退化的2-D图形表示,同时利用复向量的主要特征—模和相位,给出了蛋白质序列的一种数值刻划,进一步在3-D空间里,把20种氨基酸分别分配给正12面体的20个顶点,根据正12面体的对称性得到了20种氨基酸的3-D表示,进而得到了蛋白质序列的3-D图形表示和对应的数字序列,并利用图的不变量和数字序列的特征比较了9种动物的神经元

Durum is the hardest of all wheat and typically contains higher average protein content (12%~ 14%)than wheat of other classes. With a general shortage of protein in the world, breeders focus on improving the total amount of protein in wheat.

多伦小麦是小麦中最坚硬的,含有典型的与其它品种的小麦相比较高的平均蛋白质含量(12%~14%),由于在世界上的通常缺少蛋白质,因此种植者把重点放在改善提高小麦中的蛋白质总含量。

This desulfurization carries out floatation desulphurization experiments by using egg white and Bovine serum albumin as contrasted with microbial. The results make clear that the protein in bacteria operate mainly to the desulfurization.

在对微生物预处理浮选脱硫机理的探讨研究中,我们采用蛋清蛋白质和化合蛋白质与T.f菌进行浮选脱硫的对比试验,结果发现T.f菌中蛋白质在脱除煤中硫的过程中起主要作用。

To study the surface treatment of the quartz glass, and the coupling of the albumin protein, we used with the fictionalization of the copolymers, and experimental conditions for protein immobilization.

蛋白质固定化之制作程序系将白蛋白固定於涂有光阻剂共聚物之石英玻璃表面,然后检测蛋白质於280 nm波长附近的吸光特性,以评估蛋白质固定化的效果。

In this study, we utilized the protein technology to compare the varieties of the sarcoplasmic protein, myofibrillar protein and Urea, SDS extraction of total protein profile in the 5 species of fresh filefish, including Cantherhines dumerilii, Aluterus scriptus, Aluterus monoceros, Monacanthus chinensis and Stephanolepis cirrhifer. Then, these protein profiles were employed to identify the fish species.

本篇论文乃利用SDS-PAGE蛋白质电泳技术分析台湾产主要五种新鲜单棘魨鱼种包括杜氏刺鼻单棘魨、长尾革单棘魨、单角革单棘魨、单棘魨及冠鳞单棘魨之水溶性、盐溶性蛋白质及SDS、Urea萃取之总蛋白质图谱,比较其应用於鱼种鉴别之适切性。

In order to develop the cultivation techniques for high quality of foxtail millet , the ex-periments were accomplished in red soil, loess soil, red-loess soil and different elevations.

为了提高人民生活质量,开发优质蛋白质是优质栽培的重要内容川,谷子蛋白质是禾谷类作物中的优质蛋白质川。

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