蛋白质

In the alfalfa dry matter, crude protein content as high as 18%~ 24.8%, protein containing more than 20 kinds of amino acids, including all the essential amino acids in humans and animals, as well as One more rare amino acids (citrulline, ammonia con acid, etc.), in which lysine content of

在苜蓿的干物质中，粗蛋白质含量高达18％～24.8％，蛋白质含有20种以上的氨基酸，包括人和动物的全部必需氨基酸，以及壹些稀有氨基酸，其中赖氨酸的含量为1.06％~ 1.38％。

Results The activation of NF-κB, proliferation response, and expression of IL-4 and I L-5 mRNA and protein in T lymphocytes stimulated by PMA were significantly hig her than those of their blank control (P.01), while those indexes of T l ymphocytes stimulated by PMA and PDTC simultaneously were significantly lower th an those stimulated by PMA alone (P.01). The apoptotic index of T lympho cytes stimulated with PMA were significantly lower than that of their blank cont rol (P.01), and the apoptotic index of asthmatic guinea pig T lymphocytes stimulated with PMA and PDTC simultaneously were significantly higher than that stimulated by PMA alone (P.01). The significant positive correlations w ere found between the activation of NF-κB and the proliferation (r=0.64, P.001), and the expression of IL-4 and IL-5 mRNA and protein of T lymph ocytes, respectively (r=0.55-0.68, P.001). There was also signific ant negative correlation between the activation of NF-κB and apoptosis of T l ymphocytes (r=0.62, P.001). Conclusions NF-κB may participate in the signal conduction of PKC regulated proliferation, apoptosis and expression of IL-4 and IL-5 of T lymphocytes in asthma.

结果加入PMA培养的哮喘组T淋巴细胞NF-κB的活化、细胞增殖反应、IL-4和IL-5的mRNA和蛋白质的表达均显著高于其空白对照(P.01)，而同时加入PMA和PDTC培养的哮喘组T 淋巴细胞以上指标均显著低于只加入PMA培养的哮喘组T淋巴细胞(P.01)；加入PMA 培养的哮喘组T淋巴细胞的凋亡指数显著低于其空白对照(P.01)，而同时加入PMA 和PDTC培养的哮喘组T淋巴细胞凋亡指数显著高于只加入PMA培养的哮喘组T淋巴细胞(P 。01)。T淋巴细胞NF-κB的活化与增殖反应呈显著正相关(r=0.64，P.00 1)，与IL-4和IL-5的mRNA和蛋白质的表达也均呈显著正相关(r=0.55-0.68，P 。001)，而与凋亡指数呈显著负相关(r=-0.62，P.001)。

Again, many dieticians claim that vegetarianism is unbalanced because humans need protein, especially from animals.

要求素食主义是不合理的，因为人类对蛋白质的需求，特别是从动物身上获取的蛋白质。

Successful in-solution digestions of single proteins and complex protein mixtures were achieved in 60 s and then analyzed by reversed phase liquid chromatography-electrospray ionization ion trap-mass spectrometry.

压力循环技术成功在60sec内把单一蛋白质及复杂的蛋白质水解。然后利用反相液相色谱分析。

The classical mechanism of E2 involves estrogen binding to receptors in the nucleus, after which the receptors dimerize and bind to specific response elements known as estrogen response elements located in the promoters of target genes. However, ERs can also regulate gene expression through protein-protein interactions with other DNA-binding transcription factors in the nucleus, or through regulation with production and activity of other genes.

除了与经典的雌激素反应元件结合引起基因转录外，也可通过蛋白质-蛋白质之间的相互作用而调节其他转录因子的转录激活作用，或者对其他转录因子生成或活性的影响而调节基因转录。

Dystrophin is a large protein which is found on the inner side of the membrane surrounding each muscle fibre.

蛋白质d是一个分子量比较大的蛋白质，位于肌纤维细胞膜内侧。

Furthermore, from sodium dodecyl sulfate polyacrylamide gel electrophoresis analysis results, it was found that the protein concentration difference was reduced obviously in the eluant of direct sample loading, and most high abundance proteins were identified in the eluant of NaCl.

此外，凝胶电泳的分析结果显示，在上样流出液中蛋白质的浓度差异明显变小，大量的高丰度蛋白质存在于NaCl洗脱液中。

Dorsalis by salting out, ion exchange chromatography, and gel filtration chromatography. By salting out the egg homogenate, SDS-PAGE analysis revealed that the Vns were mainly precipitated in a 50~70% ammonium sulfate solution. The salted-out proteins were further purified by ion exchange chromatography, and the result showed that Vns were mainly eluted with a 0.3~0.5 M sodium chloride gradient. Finally, the elutes of ion exchange chromatography were refined by running them through gel filtration chromatography to obtain purified Vns. SDS-PAGE separated the 48- and 51-kDa purified Vns, and used them as antigens to raise polyclonal antibodies.

利用硫酸铵盐析法初步分离果实蝇卵中的蛋白质，以SDS-PAGE蛋白质电泳分析得知，卵黄蛋白主要在硫酸铵溶液浓度为50~70%时被沉淀出；盐析沉淀所得之卵黄蛋白，再以离子交换层析法进一步纯化显示，卵黄蛋白主要会在0.3~0.5 M氯化钠的浓度范围间被析出来；再将析出液浓缩后，经凝胶过滤层析法做最后的纯化，即获得纯化之卵黄蛋白。

GenBank data is accessible through NCBI's integrated retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome and protein structure information.

GenBank数据可以通过NCBI的整合检索系统Entrez访问，该系统整合了来自主要的DNA和蛋白质序列数据库，连同分类，基因组和蛋白质结构信息。

Entrez is the integrated, text-based search and retrieval system used at NCBI for the major databases, including Nucleotide and Protein Sequences, Protein Structures, Complete Genomes, Taxonomy, and others.

本网站是一个综合性的、全文搜索系统，使用NCBI为主要数据库，包括核苷和蛋白质次序，蛋白质结构，完全基因组，分类法和其他。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。