蛋白质

By using multi-dimensional nuclear magnetic resonance method we have studied the folding mechanism of staphylococcal nuclease in vitro; the tertiary interactions for folding of SNase fragments into native-like conformation; the interaction between SNase N- and C-terminal subunits; the relationship of enzyme activity with folding and dynamic states of SNase; the structural properties of enzyme protein while exert its function. We have studied the internal motions of thermophilic Archaea protein Ssh10b and mechanism of its heat-resistance using the NMR 1H-15N relaxation and H/D exchange methods. We have determined the 3D solution structure of human translationally controlled tumor protein TCTP and the Ca2+-binding site; determined the 3D crystal structure of human mitoNEET, a novel protein from distinct groups of iron-sulfur proteins; determined the 3D solution structure of a novel chromatin protein Cren7. Determination of SNase-DNA and Archaea protein-DNA complex structures are in progress.

运用异核多维核磁共振方法研究了金黄色葡萄球菌酶体外折叠机制，酶蛋白片段体外折叠成类天然溶液三维构象的三级相互作用力，酶蛋白亚基间的相互作用，酶蛋白的折叠以及内运动状态与酶活力的关系，酶蛋白发挥功能时的结构特性；运用NMR的1H-15N 驰豫和H/D交换方法研究了嗜热古菌蛋白质Ssh10b双体结构内运动特性，热稳定性机制；确定了人翻译控制的肿瘤蛋白TCTP蛋白的溶液三维结构及其钙离子的结合部位；确定了一类新的铁硫蛋白家族蛋白人线粒体膜上mitoNEET蛋白的晶体结构；确定了一个新型的染色质蛋白Cren7的溶液三维结构；正在研究金黄色葡萄球菌酶及嗜热古菌蛋白质与DNA复合体的溶液三维结构。

SUMO has been shown to covalently modify a large number of proteins, reversible modification by SUMO regulates nucleocytoplasmic translocalization, protein-DNA binding activity, protein-protein interaction, transcriptional regulation, DNA repair, and genome organization.

SUMO与靶蛋白之间这种可逆的共价连接，在核质运输、DNA 与蛋白质结合活性、蛋白质之间相互作用、转录调控、DNA 修复以及维持基因组稳定等方面均发挥着重要的调节作用。

In this study, nonionic surfactant sorbitan trioleate (Span 85) was modified with Cibacron Blue F-3GA to become an affinity surfactant (CB-Span 85) and to form affinity-based reversed micelles while dissolved in hexane. Since the CB molecule possesses a specific conformation that mimics nicotinamide adenine dinucleotide, it can bind strongly and specifically to a wide range of nucleotide-dependent enzymes such as dehydrogenases and kinases. Moreover, high protein transfer efficiency in the reverse micelles can be easily achieved by controlling the ionic strength of the aqueous phase.

本实验使用非离子型界面活性剂Span 85与蓝色染料分子Cibacron Blue F-3GA结合，形成具有亲和性的界面活性剂 CB-Span 85，由於CB染料分子具有与NAD+相似的结构，可与含核苷酸部位的蛋白质具有亲和力，将CB-Span 85溶於有机溶剂形成反微胞相，可有效的提高反微胞的选择率，可以只控制离子强度来调节蛋白质进出反微胞的效率。

He protein product of TSC1 is hamartin and that of TSC2 is tuberin.

SC1的蛋白质产物是错构瘤蛋白，而TSC2的蛋白质产物是结节蛋白。

We report a previously uncharacterized alpha-helical module, the BEN domain, in diverse animal proteins such as BANP/SMAR1, NAC1 and the Drosophila mod(mdg4) isoform C, in the chordopoxvirus virosomal protein E5R and in several proteins of polydnaviruses.

我们在多种动物蛋白质，如BANP/SMAR1，NAC1和果蝇mod(mdg4)异构体C，及脊椎动物痘病毒亚科蛋白质E5R和一些多去氧核糖核酸病毒科中，报道了一个以前没有描述的α螺旋结构模块----BEN结构域。

In uninduced HEL cells, GATA-2 transcription factor can specifically bind to the regulatory elements of human β-globin gene, including the proximal regulatory element (theβ-promoter) and the distal regulatory elements (the DNaseⅠ hypersensitive sites in the locus control region, HS2-HS4 core sequences).

在非诱导的HEL细胞的核蛋白中，能够与β—珠蛋白基因的近端启动子调控序列及远侧端LCR调控序列（HS2，HS3和HS4核心DNA序列）相结合的蛋白质主要是GATA—2转录因子，而在羟基脲诱导的HEL细胞核蛋白中，与上述调控序列相结合的蛋白质主要是GATA—1转录因子。

Due to the fact that water molecule acquired energy from magnetic field and the molecule rotated faster after magnetization process, the growth speed of Escherichia-coli cultivated with magnetized water is faster than that cultivated by unmagnetized tri-distilled, which proved that magnetized water possesses stronger penetrability and solvency.

蛋白质水接触的几率也随之增加，所以磁化水对于蛋白质的结构将有一定的影响。由于磁化过的水水分子活动加快，并获得了高能量，使磁化水培养的大肠肝菌生长速率比普通三蒸水培养的大肠肝菌生长速率快，这就进一步证明了磁化水具有较强的渗透性和对可溶性物质的溶解能力。

The changes include a volume-fraction correction to account for unmixable atom types in proteins and DNA in addition to the usage of a low-count correction based on bayesian statistics, residue/base-specific atom types,and a shorter cutoff distance for protein-DNA interactions.

我们在DFIRE的基础上减短了统计距离，使用了更全面的原子类型，并使用了以贝叶斯统计原理为基础的背景统计小数修正和为了区分蛋白质核酸原子类型的体积分数修正，从而得到了一种更先进的蛋白质核酸作用的基于知识的统计势函数。

Mass spectrometry driven BLAST is a sequence similarity searching tool, which has been developed in recent years and successfully applied to the identification of protein from species with unsequenced genomes.

基于同源性搜索的BLAST方法，是近年新发展起来的一种用于未知基因组的蛋白质鉴定的搜索工具，已成功应用于许多未知基因组物种的蛋白质鉴定。

Here, we show that DEG5 and DEG8 form a hexamer in the thylakoid lumen and that recombinant DEG8 is proteolytically active toward both a model substrate and photodamaged D1 protein of photosystem II , producing 16-kD N-terminal and 18-kD C-terminal fragments.

我们研究发现在类囊体膜腔侧的DEG5和DEG8形成6聚体，重组的DEG8对于模式底物和光破坏了的光系统Ⅱ中的D1蛋白质都有蛋白酶解活性，可以将D1蛋白质剪切成N-末端16kD和C-末端18kD的片段。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。