蛋白质

Nonfouling mechanism of polymer materials and recent research progress of protein-resistant polymer preparation and surface modification are reviewed.

本文对聚合物材料表面阻抗蛋白质吸附的机理进行了归纳，介绍了阻抗蛋白质吸附的材料制备及表面修饰的相关研究进展。

Then they examined PTEN in nonhereditary breast tumors from 297 patients and tumors from 34 women who had inherited the BRCA1 mutation. In most cases, the protein that PTEN produces was undetectable in tumor cells, but clearly present in nearby normal cells.

然后他们检查了297名患非遗传性乳房肿瘤的妇女和34名遗传了BRCA1变异并患肿瘤的妇女，发现在大多数情况下，肿瘤细胞里检测不到由PTEN产生的蛋白质，但在附近正常细胞的周围明显存在由PTEN产生的蛋白质。

The results were as follows the change of vitamin C content was a "trial apex"-shape curve, higher in young fruit,incrustation and maturation stages, lower in other periods; the total acid content was the highest in young fruit, after that, it decreased rapidly, maintained lower level, and reached lowest when the fruit matured; the total content of soluble sugar inosculated with the fruit development, it was high when the fruit development was quick, and low when the fruit development was slow; before 59 d after anthesis, the reducing sugar content was high in the total content of soluble sugar and after that ,the nonreducing sugar content was high; before 45 d after anthesis, free amino acid content was obviously correlated with protein content, and they inosculated with the fruit development;4559 d after anthesis, free amino acid content increased quickly, and protein content decreased gradually, this was also the most shaping period of the embryo and endosperm.

结果表明，Vc含量呈&三峰&型曲线，幼果时、硬核后和成熟时较高，其他时期较低；有机酸含量以幼果期最高，以后迅速下降并维持较低水平，果实成熟时含量最低；可溶性总糖含量与果实发育状况基本相吻合，果实发育速度快时含量较高，慢时较低；在谢花后59 d以前，可溶性糖以还原糖为主，之后以非还原糖为主；游离氨基酸和蛋白质含量，在谢花后45 d以前两者呈高度相关，且与果实生长速度相吻合，谢花后4559 d是胚和胚乳的主要形成期，此期游离氨基酸含量迅速增加，蛋白质含量持续下降。西北林学院学报21卷第5期张传来等金光杏梅果实发育期间主要营养成分动态变化研究

The fiber components determined included nonstarch polysaccharides, lignin and ploy-phenols, cell wall protein, and minerals. On average, in comparision to Canola meal, Chinese double-low rapeseed meals contained the similar or more amounts of sucrose,CP and lysine,but the much different amounts of DF,in particular,the cell wall protein,which depended on the processing methods. In addition.the content of glucosinalates varied amoug the sample. Comparing with the corresponding de-fatted seed samples, the amounts of sucrose,lysine and dietary fiber varied obviously in terms of processing technolngy.

饲粮总纤维由非淀粉多糖、木质素和多酚、细胞壁蛋白质和矿物质组成。3个中国双低菜粕的粗蛋白、蔗糖及必需氨基酸含量均接近或高于卡诺拉帕，但饲粮总纤维含量显著不同，尤其是蛋白质含量由于加工方式不同变异极大，在试验室条件下加工的脱脂种籽样品与相应的饼粕样品相比，蔗糖、赖氨酸和饲粮纤维含量变化显著。

These observations strongly indicate that Lyar is a critical nucleolar protein in regulation of ES cell proliferation, cell cycle, and survival.

为研究Lyar对ES细胞增殖作用的机制，我们利用蛋白质亲和层析方法寻找与Lyar相互作用的蛋白质。

Objective:To study the difference between the normal tissue and carcinoma of ovary,set up the proteome atlas of ovarial carcinoma then lay a foundation of early diagnosis in clinic.

目的：研究卵巢正常、恶性肿瘤组织蛋白质表达的差异，构建卵巢恶性肿瘤组织的蛋白质表达谱，为临床早期诊断奠定基础。

The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect.

通过比较发现3种蜚蠊的蛋白质区带数目和位置有差别；德国小蠊主要蛋白质区带的位置与两种大蠊相差明显；♀♂之间也有差别。

The structure and form of peritrophic membraneof Helicoverpa armigera were observed by electron microscopy.The type of protein,the content of protein and carbohydrate in PM were analyzed.

应用电子显微技术和生化技术，研究了棉铃虫Helicoverpaarmigera围食膜的超微结构和表面形态，初步分析了围食膜蛋白质的种类，测定了蛋白质和糖的含量。

To avoid the permutable model of protein, the 43 Table of Triplet Codes Given by F.H.C.Crick in 1969 is in fact that one group of permutations versus the permutation elements in another group of permutations , which is a typical wrong application and a wrong calculation.

Crick 43 遗传密码表回避蛋白质分子的排列组合模型问题，根据氨基酸种类数20 和 DNA 上碱基种类数 4进行对四元素选三元素的排列组合计算建立& DNA ～蛋白质&间的一一对应，本质上是排列组合应用中一组排列组合对应另一组排列组合的具体元素。

After the cell growth curves was recorded, RPE cells of the 3-5th passages were utilized. 2、Three different siRNA (siRNAl,siRNA2,siRNA3) targeting against human cx43 gene and one negative control siRNA were designed and transfected into cultured human RPE cells via liposome reagent. The most effective siRNA can be determined by semi-quantitative reverse transcription PCRRT-PCR. 3、To the most effective siRNA, after transfected into human RPEs with different concentration, the cellular proliferate activities were messured by MTT colorimetry ; the percentages of RPE in different cell circle phase was assayed by FCM; the changes of phenotypical properities were observed with SCM; the protein expression of cx43 was studied through immunocytochemistry stain and Weston blot; the communication intercellular was calculated with FRAP; and the ability of recovery was assessed by using an in vitro wound healing model.4、The total proteins of siRNA1 and RPE were seperated by two-dimensional gel electrophoresis and visualized by silver staining. Proteins with significant expression alterations were selected and their peptide mass fingerprints (PMFs were obtained by matrix-assisted laser desorption/ionization time of flying mass spectrometry (MALDI-TOF-MS).The PMFs were used to search NCBInr database by Auto MS-Fit software.

实验方法：1、培养原代的人RPE细胞，经过细胞角蛋白、S-100和神经胶质原纤维酸性蛋白免疫细胞化学鉴定后，通过AO/PI染色技术确定培养细胞的存活率，描记其生长曲线，第3-5代用于以下细胞实验2、生物合成针对人cx43基因的三条小干扰RNA和一条阴性RNA通过脂质体转染RPE细胞后，通过RT-PCR的方法确定抑制效率最高的干扰片断3、将该片段以不同浓度通过阳离子脂质体转染培养的人RPE细胞后，采用MTT法观察其对细胞的增殖力的作用；通过流式细胞仪观察其对细胞周期的影响；通过扫描电镜观察其对细胞形态的影响；通过免疫细胞化学和Weston blot观察其对cx43蛋白表达的作用；采用激光共聚焦和荧光淬灭恢复技术观察荧光恢复速率平均百分率，评价其对细胞间通讯功能的影响；通过制作RPE细胞损伤模型，观察其对损伤修复能力的作用4、分离纯化转染siRNA的RPE组和正常对照组RPE细胞的全部蛋白质，应用等电聚焦电泳和SDS-PAGE双向电泳技术，银染显示分离出的蛋白质斑点，经凝胶图像分析软件对两个样本进行胶图分析，寻找差异蛋白点。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。