蛋白质

The EBA of protein with the anion exchanger Streamline DEAE was examined by investigating the effect of liquid flow rate, liquid viscosity and protein concentration. A modified mathematical model was established to describe protein adsorption behavior in the expanded bed chromatography by taking into account the axial size classification of the adsorbent within the bed.

研究了阴离子交换吸附剂 Streamline DEAE 的蛋白质吸附特性及其膨胀床的蛋白质动态吸附行为，考察了液相粘度、流速和蛋白质浓度对色谱穿透曲线的影响。

The results showed that:(1) The relative weight gain, special growth rate, feed conversion rate, feed protein efficiency and protein deposition rate were improved significantly (P.05) by supplementing compound acidifier in common carp diet.(2) The protease activities in the liver pancreas, fore and middle intestines increased significantly (P.05) by supplementing 0.3% compound acidifier. The activities of amylase and lipase were improved after the supplementation, but not at significant level.(3) Supplementation of compound acidifier in common carp diet did not significantly affect the fish body protein, lipid, ash, moisture contents, coefficient of fatness and viscerosomatic index.

结果表明：1添加复合酸化剂显著(P.05)提高鲤鱼的相对增重率、特定生长率、饵料转化效率、饲料蛋白质效率以及饲料蛋白质沉积率等生长性能指标；2添加质量分数0.3%的复合酸化剂时，鲤鱼肝胰脏、前肠和中肠的蛋白酶活性显著提高(P.05)，酸化剂组的淀粉酶和脂肪酶活性有升高的趋势，但与对照组差异不显著；3鱼体的粗蛋白质、粗脂肪、粗灰分、水分、肥满度及内脏比等品质指标在各组间差异不显著。

During the processes of conditioning and pelleting, decrease of particle...

调质和制粒过程中，小粒度有利于蛋白质变性和淀粉糊化，使蛋白质体外消化率和淀粉糊化度提高；增加水分有利于淀粉糊化，而对蛋白质变性、VC活性减小和有害微生物灭活的影响较小；升高温度有利于蛋白质变性和淀粉糊化，使VC活性减小和有害微生物灭活；在高温调质和制粒过程中，饲料中的绝大部分致病菌[0]可被杀灭。

The objectives of this study are:(1) To amplify Tb wbbL gene encoding rhamnosyl transferase bypolymerase chain reaction from the genomic DNA ofM.tuberculosis H37RV strain;(2) To clone PCR product of Tb wbbLgene into a cloning vector pMD18-T for sequencing;(3) To subcloneTb wbbL gene to an expression vector pET16b to construct pET16b-Tb wbbL; and to overexpress Tb WbbL protein in E.coli BL21(DE3)under different induction conditions;(4) To establish theco-expression system for expressing chaperons of pKJE7 plasmidand soluble Tb WbbL protein in E.coli BL21(DE3) under differentinduction conditions;(5) To test expressed WbbL protein by SDS-PAGEand Western blot methods.

本论文的目的是：(1)利用 PCR 方法从结核分枝杆菌 H37Rv 菌株的基因组DNA 中扩增出 Tb wbbL 基因；(2)将 Tb wbbL 基因克隆到pMD18-T 克隆载体中，经 DNA 序列测定证实为正确的基因；(3)将 Tb wbbL 基因亚克隆到 pET16b 表达载体中并通过改变不同的诱导条件在大肠杆菌 BL21(DE3)中表达 Tb WbbL 蛋白质；(4)建立在 BL21(DE3)大肠杆菌中共表达 Tb WbbL 蛋白质与分子伴侣的体系，优化表达条件以高效表达出可溶性 Tb WbbL蛋白质；(5)用 Western blot 方法鉴定所表达的 Tb WbbL 蛋白质为 Tb wbbL 基因产物。

By using lysozyme, RNase A and protein disulfide isomerase as model proteins, we present a hypothesis that protein cross-linking can be accomplished in three concerted steps:(1) a change in protein conformation;(2) formation of interchain disulfide bonds; and (3) formation of interchain isopeptide cross-links.

我们以溶菌酶、核糖核酸酶A和蛋白质二硫键异构酶为模型对异肽键进行了体外详细的研究，提出了解释异肽键形成机理的假说：1蛋白质发生构象变化包括二级结构的改变；2蛋白质形成分子间二硫键：3二硫键的形成帮助蛋白质形成了分子间异肽键。

Transport signal in a soluble cargo protein (for example, a secreted protein that passes through the lumen) is a region that binds to the lumenal domain of a transmembrane cargo receptor, which in turn has an cytoplasmic domain that binds an adaptor protein. Interaction between the cargo and the coat

我们已经有了几种类型的信号的细节：要求一种构像用于蛋白质通过胞吞作用成为细胞内的一部分；一种氨基酸序列使得蛋白质到达内质网；和一种修饰使得蛋白质到达溶酶体（小的膜体，在那里蛋白质降解，见后面）。

Prediction of protein subnuclear locations becomes an important research hot point in protein science.

随着蛋白质数据库中蛋白质序列数量的急速增加，采用计算方法来预测蛋白质亚核定位已经成为蛋白质科学领域研究的热点。

The prediction equations of nutritional requirements about digestible energy, metabolizable energy, digestible crude protein, and protein deposited for stall-feeding growing crossbred Boer goats were:DE, kJ/d=588.31W0.75 9.25W 879.9 r=0.5712, p=0.013ME, kJ/d=482.42W0.75 7.57AW 721.57 r=0.5713, p=0.013DCP, g/d=(0.03AW 1.27) W0.75 r=0.9869, p=0.0131 PD, g/d=(0.03W 0.03) W0.75 r=0.9993, p=0.0007 When the average daily gain stage is from 0 to 200g/d,for the stall-feeding growing crossbred Boer goats, aged at 3~6 months, Metabolic rate of gross energy of the diets is 0.55; digestibi I ity of crude protein is 0.56; The protein requirement of per live weight gain for meat goats is 0.31 g PD/g gain .

舍饲波杂肉羊生长期消化能、代谢能、可消化粗蛋白质、沉积蛋白质需要量的估测模型分别为： DE，kJ／d=588.31W~(0.75) 9.25△W 879.9 r=0.5712，p=0.013 ME，kJ／d=482.42W~(0.75) 7.57△W 721.57 r=0.5713，p=0.013 DCP，g／d=(0.03△W 1.27)W~(0.75) r=0.9869，p=0.0131 PD，g／d=(0.03△W 0.03)W~(0.75) r=0.9993，p=0.0007 3～6月龄舍饲生长期波杂肉羊增重范围为0～200g／d时，日粮总能代谢率平均为0.55，粗蛋白质的消化率平均为0.56；每增重1g体沉积蛋白质需要量为0.31g。

Methods Conjugated with protein precipitation with trichloroacetic acid, BCA method was used. According to the positive relationship of protein content with the 570 nm absorbance, protein content was calculated in powdered milk.

使用三氯乙酸沉淀蛋白质后，运用BCA法，在570nm波长处，分别测定标准蛋白质应用液与样品稀释液的吸光度值，基于测定液中蛋白质含量与其吸光度值呈正比关系，计算出样品中蛋白质的含量。

The aim of the USN network is to clarify the function and structure of certain protein complexes, while the remit of the SMP Protein project is to investigate disease-related protein-protein interactions on a molecular basis.

超微结构网研究计划的目标就是彻底了解确定蛋白质复合体的功能和结构，而系统方法平台蛋白质计划的目标则是在分子基础上对与疾病相关的蛋白质与蛋白质相互作用进行研究。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。