蛋白质

And the necessary of off lattice model and simplified energy function in protein folding is get. Then energy computation of protein folding process is designed and implemented. Secondly, with the characteristics of robustness and the ability of searching the better optimization, genetic algorithm, ant colony optimization algorithm and particle swarm algorithm are researched.

基于这个问题，主要在以下几个方面进行研究：首先，分析蛋白质折叠模型和能量优化函数，得出蛋白质非格点模型和简化能量函数在蛋白质折叠研究中的必要性，并设计实现基于非格点模型的蛋白质折叠的能量计算模块。

Coli can overproduce 38 000 antigen of Mycobacterium tuberculosis. Inclusion bodies are easy to purify and can protect the recombinant antigen from protease, on the other hand, it has not biological activity unless the denatured protein is accurately folded.

构建的大肠杆菌重组体能高效表达结核分支杆菌38 000蛋白质抗原，包涵体的表达形式有利于蛋白质纯化和蛋白质稳定，蛋白质必须经过正确折叠才具有生物学活性。

In Panicum repens grown naturally in water marsh, the content of 39.1,68.5 and 130 kD polypeptides was high, and under NaCl stress, the three proteins decreased somewhat, but the 30.6 kD polypeptide was enhanced along with salinity increase. The 30.6 kD protein may be related to salt acclimation of Panicum repens.

移栽后的植株在50 ～400 mmol/L NaCl胁迫下，原生长于海滩的铺地黍中30.6、73.5和96.5 kD蛋白质的含量随盐度的增加而增加；原生长于水湿地的铺地黍中39.1、68.5 和130 kD蛋白质的合成降低，但30.6 kD的蛋白质被诱导合成。30.6 kD的蛋白质可能与铺地黍的盐适应过程有关。

A method for the determination of BSA based on the quenching of BSA fluorescence on adding PS and a method for the determination of PS based on the enhancement of PS on adding BSA were developed. The experimental data were processed by the Stem-Volmer equation and Lineweaver-Burk double reciprocal equation.

在不同的pH条件下，酚藏花红与蛋白质的反应导致了蛋白质荧光猝灭及酚藏花红的荧光增强，其荧光猝灭值与酚藏花红的浓度成正比，可用于酚藏花红的分析测定；而酚藏花红荧光增强值与蛋白质浓度成正比，又可用于蛋白质的分析测定。

Since Anfinsen and his co-workers brought forward the plus of the stability of thermodynamics is the right folding drive in the protein folding process basis on the study of the reversible ribotide-enzyme stretch and refold in 1960s, the investigation on the study of kinetics and thermodynamics of protein folding and binding have been greatly promoted.

自从上世纪60年代Anfinsen和他的合作者根据核糖核酸酶可逆伸展与重折叠的研究提出蛋白质折叠过程在热力学稳定方面获得的增益是控制蛋白质在体外正确折叠的驱动力以来，关于蛋白质折叠的动力学和热力学研究取得了很大的进展，特别是Wolynes所建立的能量形貌图理论对生物物理学的蛋白质折叠问题提供了强有力的理论模型。

As high activities in CS reflecting aerobic nature of the tissue, the present dataset suggests that the swim bladder muscles should involve with sound production. Proteomics analyzes identified 14 proteins from the swim bladder muscle, one of them are related to energy production. Two-dimensional gels show some different spots from these three type muscles. These results help us to find some different proteins in these three types of muscles of this fish.

以蛋白质二维电泳分析鱼鳔附属肌肉，共鉴定出14种蛋白质，其中一种与能量相关的蛋白质与体侧肌的蛋白质表现不同，而此蛋白质表现与尾部红肌相似，此结果可望帮助我们找到鱼鳔附属肌肉与其他不同类型肌肉的相异之处。

Shaw's research agenda that combines major advances in computational algorithms and architecture to provide the requisite thousand-fold increase in processing speed to simulate protein folding and protein-protein interactions in a biologically meaningful timeframe.

这是生物学上崭新的一页。萧研究的课题是综合计算机算法和信息技术学的主要成果，以提供处理蛋白质折叠，加速模拟生物学"蛋白质折叠和蛋白质-蛋白质交互作用"。

The ubiquitin proteolytic system is an important selective proteolytic system of the celler proteolytic systems, a number of them were identified as a ubiquitin chain assembly factor-E4. The U-box protein is a new family ubiquitin-protein ligase(E3), which determine the substrate specificity of ubiquitination. The U-box is a domain of 70 amino acids that is highly conserved among yeast, plant and animals, but more U-box proteins were found in plants than in animals.

泛素系统是选择性降解细胞内蛋白质的重要系统之一，U-box蛋白质是此系统中决定底物特异性识别的一种新型E3蛋白质，部分U-box蛋白质属于泛素链聚集因子-E4.U-box结构域大约由70个氨基酸残基构成，在酵母、植物和动物等真核生物中保守存在，但植物中的数目远多于动物中。

In the process of root cutting, the root accumulated a lot of soluble sugar and soluble protein, which provided material and energy for the cell differentiation. As root differentiating, soluable protein was continuously used up. During 10 to 20 days, the content of soluble sugar and soluble protein in the basipetal top and end of rootkept comparatively stable, then declined as adventitious buds and roots growing. 40 days later, they kept invariable, because the newly formed bud can photosynthesize and the newly formed root can absorb nutrient from outside. The dynamic balances of endogenous hormone regulate adventitious buds and roots of the root. During callus growing and adventitious bud differentiating.

上部根段在细胞分化前期积累了大量的可溶性糖，为后期的分化提供能源物质，同时积累了大量的可溶性蛋白质，为愈伤组织的产生提供能源，而后随着分化，可溶性蛋白质不断被消耗。10～20d根段上部及下部的可溶性糖、可溶性蛋白质含量保持在相对稳定的水平变化不大，后期随着不定芽及不定根的生长下降较快。40d后，新形成的芽已经能够进行光合作用，新形成的根也可从外界吸收营养物质，可溶性糖、可溶性蛋白质含量基本保持不变。

In this review,we introduced the concept of protein turnover and the importance of its measurement.Both the direct and indirect methods were reviewed,and the compartmental analysis method was discussed in detail.

本文对蛋白质周转代谢的概念、术语、动物蛋白质周转代谢的意义做了阐述，介绍了蛋白质周转代谢的测定方法，并重点介绍了蛋白质周转代谢的随机分析与房室分析方法。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。