蛋白质

Some previous experiments have shown that areca ingredients can cause damages of tissue and oral cells and the changes in expression of some proteins. But there is no research using high throughput strategies to establish the proteomic data.

在先前的研究中虽已发现槟榔对细胞及组织的伤害和诱导某些蛋白质表现量上的差异，但并没有以高产能的方式探讨其对细胞中蛋白质表现的直接影响。

We find out that TCA / acetone method suitable for extraction of total protein upon Areca leaves through compare to different methods of protein extraction. It is the base to get good 2-DE map.

通过优化双向电泳的条件（包括电泳过程中电压的选择、第一向和第二向温度的控制、准确的定量蛋白质、优化蛋白质的上样量、裂解液的选择等），获得了较高质量的2-DE图谱。

The proteomics research of Artemia sinica has gradually become one important post-genome research focus.

本研究在建立了高质量的蛋白质组学研究平台的同时，还对卤虫胚胎在不同发育组织时期的蛋白质构成进行了调查研究。

Part one: Previous results indicated that the abnormal development of haploid goldfish embryos is due to the expression obstruction of some proteins, which are correlative with eyes development, nerve development, development regulation, cell differentiation and maintenance, arthromere formation and signal transduction.

第一部分金鱼雌核发育过程中胚胎蛋白质组比较研究前期已发表的工作表明在金鱼雌核发育眼睛形成期(受精后26个小时)单倍体上一些与眼睛发育、神经发育、发育调控等相关的重要蛋白质未能被选择表达或表达受阻，从而导致单倍体发育畸形。

Additionally, as to the comparison of protein structures,we propose a "symbol assignment" approach,which can translate protein structures into symbol sequences.

另外，对于蛋白质结构的比较，我们提出了一种"符号化指派"的方法，可将蛋白质结构的比较转换为符号序列的比较。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明：猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同，均属不稳定类酶蛋白；保守结构域氨基酸序列具有高度的同源性，均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序；均有信号肽，属分泌型蛋白质；属于跨膜的疏水性蛋白，均有5个跨膜结构域；无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件；三维结构预测都呈"V"字形；猪和牛HMGR基因的进化关系最近。

Proteins were prepared by the combination of various extraction methods based on the method of inducer plus autolysis from waste brewer yeast .

本研究在采用诱导剂+自溶法的基础上，结合多种提取方法，通过对比和正交试验确定啤酒废酵母中蛋白质的最佳提取工艺及参数，并用胃蛋白酶-胰蛋白酶联合消化法测定提取蛋白质的消化率。

Two general reasons are most commonly given to support the reduction of dietary protein in animals with renal disease. First, reduced protein may result in reduced azotemia, which limits the nausea of renal failure and allows animals to continue eating.

对於有肾脏疾病的动物，有两个普遍的理由用来支持减少蛋白质饮食摄取：第一、减少蛋白质可减少氮血症发生，可限制因肾衰竭所造成的恶心感，使动物可继续进食。

After being sieved, the cottonseed meal was processed by density fractionation with K 2CO 3 solution for those ones the diameter in 105～220,220～355,355～450 μm and bigge...

采用筛分和液选相结合的方法可以得到蛋白质含量为 6 6 %的棉籽浓缩蛋白，蛋白质收率 75 。2 %。

In this study, we show that myotonia congenita-related mutations influence the biogenesis and the subcellular localization of hClC-1 channels.

本研究中证实与先天性肌肉强直症相关之突变会影响hClC-1蛋白质的表现总量与蛋白质分布型态，为先天性肌肉强直症的致病机转提供一个新的方向。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。