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蛋白质

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Some parameters that affect the properties and yield of hydrolysate, such as the amount of the prote...

研究结果表明:采用此方案 Cr2 O3在水解蛋白质中的含量仅为 10 -6 级;水解蛋白质的回收率为 4 5 %左右,蛋白质总回收率达到 85 %左右

Protein degradation in the rumen is the result of microbial activity and affects by physical and chemical characteristics of protein and the characteristics of rumen and ruminal digesta.

蛋白质在瘤胃内的降解由瘤胃微生物来完成,蛋白质的物理和化学特性、瘤胃及其内容物的特性均影响蛋白质降解。

The sulfamate moiety in the structure of topiramate is chemically related to known CA inhibitors, and in fact, topiramate inhibits CA activity. To further explore the pharmacological action of topiramate, and clarify the possibility of anti-tumor growth and metastasis of CA inhibitors and its relation with AQP1, we studied the effect of topiramate on adhesion of high metastatic prostate carcinoma to laminin, and established the spontaneous metastatic model of Lewis lung carcinoma to studied the influences of topiramate on tumor growth and metastasis and AQP1 expression in lungs and primary tumors of mice.

为了深入研究托吡酯的药理作用,阐明CA抑制剂和水通道蛋白AQP1相互作用影响肿瘤生长和转移的可能性及其机制,我们研究了托吡酯对高转移的前列腺癌细胞粘附于基质成分层粘连蛋白的影响;以Lewis肺癌自发性转移为模型,考察了托吡酯对肿瘤生长和转移的影响;同时观察了托吡酯对荷瘤小鼠肺组织及原发瘤组织AQP1表达的影响;检测了其对血管新生的作用;利用体外AQP1表达模型,研究了CA抑制剂对AQP1功能的影响;此外,用蛋白质组学的关键技术,通过对模型组与托吡酯治疗组血清蛋白质组的比较分析,来寻找与肿瘤转移相关的蛋白质,探索托吡酯的可能作用靶点。

In this research ex- tensive varieties of plant seed samples were collected and screened by analysis of amino acid co- mposition.

植物种子蛋白质中氨基酸含量不均衡[2],在食用这类蛋白质时,人体以含量最少的一种必需氨基酸为基点,按比例吸收其它各种氨基酸来组成机体蛋白质

Then main methods and algorithms involved in side-chain constructing and loop-region constructing of homologous protein are discussed,and major algorithms that are concerned in conformation search of unhomologous protein are analyzed and compared.

首先介绍了蛋白质结构预测中的三种理论方法,然后对同源蛋白质结构预测中侧链构造和环区构建中涉及到的主要方法进行了探讨,对非同源蛋白质结构预测中空间构象搜寻涉及到的主要算法进行了分析比较。

However,it is already possible to combine the advantages of high-through put proteomics in studying endoge-nous,untagged proteins in cell populations with the advantages of studying FP-tagged proteins at the single-cell level using fluorescencemi cros-copy.

然而,在研究内源性方面,已经尽可能结合高通量蛋白质的优势,细胞群体中未被标记的蛋白质利用在单细胞水平研究FP标记的蛋白质的优势使用了显微荧光交叉拷贝。

The experimental result showed that 1086 and 848 proteins were identified by MALDI-TOF-TOF and ESI-Q-TOF respectively,wherein 633 proteins were homolog;38 and 33 pro...

结果表明,用MALDI-TOF-TOF和ESI-Q-TOF方法分别鉴定出1086种和848种蛋白质,其中相同的蛋白质为633种;MALDI-TOF-TOF和ESI-Q-TOF方法分别找到38种和33种表达差异在0.5倍以上的蛋白质,其中3种为相同的蛋白质

The experimental result showed that 1086 and 848 proteins were identified by MALDI-TOF-TOF and ESI-Q-TOF respectively, wherein 633 proteins were homolog; 38 and 33 proteins were found to be differentially expressed by more than 0.5 fold by MALDI-TOF-TOF and ESI-Q-TOF respectively, wherein 3 proteins were the same proteins.

结果表明,用MALDI-TOF-TOF和ESI-Q-TOF方法分别鉴定出1086种和848种蛋白质,其中相同的蛋白质为633种;MALDI-TOF-TOF和ESI-Q-TOF方法分别找到38种和33种表达差异在0.5倍以上的蛋白质,其中3种为相同的蛋白质

The PrP of bastard halibut consists of 472 amino acid residues, weighing about 49.6kD. The deduced amino acid sequence is 71.9% similar to that of Japanese pufferfish and 66.4% similar to that of Atlantic salmon, and bears the structural signatures of PrPs including a signal sequence, tandem repeats, a hydrophobic region, two glycosylation sites, a disulphide bridge and a glycosyl phosphatidylinositol anchor site.

推测的牙鲆蛋白质感染因子蛋白由472个氨基酸组成,分子量约49.6kD,具有信号肽、短肽顺接重复、疏水区、糖基化位点、二硫键、糖基缩醛磷肌醇锚定位点等蛋白质感染因子蛋白特征结构域,与红鳍东方鲀和大西洋鲑蛋白质感染因子蛋白的相似性分别为71.9%和66.4%。

Methods: Serum samples were collected from 146 cancer patients who were treated in our studying group from April to December, 2004. Serum samples were added to a weak cation exchange 2 (WCX2) protein chip and analysed on the Protein Biology System surface enhanced laser desorption ionisation time of flight mass spectrometer (SELDI-TOF-MS) manufactured by Ciphergen Biosystems Inc.

人类肿瘤蛋白质组的研究是目前恶性肿瘤研究的重要方法之一,利用蛋白质飞行质谱技术(SELDI-TOF-MS)检测某种肿瘤蛋白质组的质谱变化,用于肿瘤的早期发现,已有不少报道[1]。

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