蛋白质

I found that the major distinction for gene duplicability in protein complexity is between hetero-complexes, each of which includes at least two different types of subunits, and homo-complexes, which include monomers and complexes that consist of only subunits of one polypeptide type.

接下来，我利用功能性基因体及蛋白质结构资料，探讨蛋白质复杂性（蛋白质次单元种类的数目，protein complexity）对基因的可移除性及可复制性的影响。

The model of Protein Secondary Structure based on the multi-modal neural network This paper proposed a multi-modal neural network predictable model that synthesizes by 7 BP neural network group in protein secondary structure prediction, this model use "profile" encoding method and "the distance" between the amino acid for the excitory input information, has carried on the prediction to the protein secondary structure prediction.

3基于多神经网络的蛋白质二级结构预测模型论文提出了一个由 7 个 BP 神经网络组合成的多神经网络的蛋白质二级结构预测模型，该模型采用&profile&编码方式和氨基酸间的&距离&为激励输入信息，对蛋白质二级结构进行了预测。

Simultaneously, the total extractible myoglobin decreased and the proportion of metmyoglobin increased significantly when the pressure above 400MPa. Beef discoloration after pressure processing may result from oxidation of ferrous myoglobin to ferric metmyoglobin, globin denaturation and structural changes. At 40℃, the changes of beef color was similar to that of room temperature's. When the temperature rise to 60℃, most of muscle proteins denatured, a small quantity of native myoglobin could not affect the color of beef significantly. Therefore, protein denaturation was attributed to the main cause of discoloration in beef muscle after pressurization at 60℃.

压力处理导致色泽变化的直接原因可能是由于二价铁的肌红蛋白氧化成三价铁的高铁肌红蛋白、球蛋白的变性及结构的变化所致。40℃时的压力处理，色泽的变化规律与室温下相似，只是色泽变化的程度更为剧烈，蛋白质变性在色泽变化中起到的作用逐渐加强。60℃下的压力处理，绝大部分蛋白质发生变性，少量未变性的肌红蛋白对色泽不会产生明显的影响，此温度的压力处理，蛋白质变性是造成肌肉失去红色的主要原因。

The bacterium Flavobacterium columnare is the pathogen causing columnaris disease, a world-wide bacterial fish disease causing heavy economical losses in aquaculture industry, and is a pathogen in China causing bacterial gill-rot disease, a serious disease in several major cultured fish species including the grass carp Ctenopharyngodon idellus .

本研究以柱状黄杆菌和草鱼为对象，以蛋白质组学技术为手段，用比较蛋白质组学的方法研究了柱状黄杆菌强毒株和弱毒株差异表达的胞外蛋白，研究了柱状黄杆菌外膜蛋白和草鱼鳃粘液的蛋白质组。

The high pressure effects on the structure properties of glycinin and β-conglycinin are analyzed and the mechanism of high pressure denaturation protein is clarified; By simulating the real food systems, high pressure effects on the interaction of protein and polysaccharide are studied; The relationship between the change of protein structure and function are studied in the presence of polysaccharide.

研究超高压处理对大豆豆球蛋白和β—伴球蛋白分子结构特性的影响，了解超高压促使蛋白质变性的机理；模拟食品真实体系，研究超高压对蛋白质及多糖相互作用的影响；分析在多糖存在的条件下，超高压处理对蛋白质分子结构变化及功能特性的影响。

Quality forage has much higher content of protein and lysine than conventional one. Generally, the content of protein in the gramineous is ranged from 13 percent to 15 percent and from 18 percent to 24 percent in the legume.

牧草蛋白质含量和赖氨酸含量都比秸秆等传统饲料高，一般禾本科干草的蛋白质含量为13%～15%，豆科牧草为18%～24%，并且牧草中所含的蛋白质更容易被家畜消化吸收。

Quality forage has much higher content of protein and lysine than conventional one. Generally the content of protein in the gramineous is ranged from 13 percent to 15 percent and from 18 percent to 24 percent in the legume.

牧草蛋白质含量和赖氨酸含量都比秸秆等传统饲料高，一般禾本科千草的蛋白质含量为13%～15%，豆科牧草为18%～24%，并且牧草中所含的蛋白质更容易被家畜消化吸收。

In this study, isobaric tags for relative and absolute quantification labeling coupled with two-dimensional liquid chromatography-tandem mass spectrometry (2D-LC-MS/MS) was employed to compare the differential protein expressions in MCF7 cells upon 17β-oestradiol (E2) stimulation or serum deprivation versus in normal conditions. A total of 576 proteins with the confidential level of ≥95% were identified, 26 proteins among the 576 proteins were found to be differentially expressed after E2 stimulation or serum deprivation, and among them 10 proteins were up-regulated and 16 proteins were down-regulated by more than two folds.

利用四重用于相对和绝对定量的等量异位标签(isobaric tags for relative and absolute quantification， iTRAQ)标记结合二维液相色谱-串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry， 2D-LC-MS/MS)对雌二醇(17β-oestradiol， E2)和血清各自以及共同作用对MCF7乳腺癌细胞内蛋白质表达的影响进行了比较，共鉴定到置信度在95%以上的蛋白质576种，其中各组相对于正常培养组的细胞共找到26种差异在1倍以上的蛋白质，其中10种上调，16种下调。

Methods:rThe protein from persons'ovarial carcinoma tissue and normal ovary was drawn,and the spots of protein were identified with the Swiss-Prot database.

取人卵巢正常及恶性肿瘤组织，提取蛋白质，进行双向电泳分离，银染获得其蛋白质电泳分离图谱对蛋白质斑点进行初步鉴定。

It was reported an cDNA encoding HMG protein was cloned from Pharbitis nil.

HMG蛋白质含有一非常保守的区域HMG-box，为确定裂叶牵牛中是否有其它含HMG-box的蛋白质以及与HMG1的关系，我们根据已知的大豆、裂叶牵牛HMG设计出引物，利用RT-PCR扩增技术、Southern、Northern杂交技术，从裂叶牵牛中克隆出编码HMG蛋白质的另一个cDNA-HMG2，它与HMG-1同源性很高(氨基酸序列同源性〉80％)，并对HMG1／2与裂叶牵牛开花功能的关系进行了研究。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。