蛋白质

In the second part of the thesis, we developed a very effective assay for the sulfotransferase that catalyzes the sulfation of serine and threonine. It utilized PAPS regenerated from 3-phosphoadenosine 5-phosphate by a recombinant PST using 4-methylumbelliferyl sulfate as the sulfuryl group donor. The change in the fluorescence intensity of 4-methylumbelliferone corresponded directly to the amount of active protein sulfotransferase.

第二部份则是探讨蛋白质亚硫酸转移酵素的活性检测方法，检测系统的主旨是用重组的PST催化受质4-methylumbelliferyl sulfate做为亚酸基提供者并转移至3'-phosphoadenosine 5'-phosphate再生PAPS ，此核苷酸的亚酸基再由蛋白质亚硫酸转移酵素催化，反应后所产生的4-methylumbelliferone之萤光变化可用来定量蛋白质亚硫酸转移酵素的活性。

Thus,the identification of the protein superfamily becomes increasingly important for protein function study.

因此，蛋白质超家族的模体特征分析及蛋白质超家族的识别对研究蛋白质结构和功能具有重要意义。

Objective: Extracting the total protein from scoipii tegument, investigating its effect on immune system by transformation of T/B cell. Method: Water-soluble protein was extracted by distilled water method and salting-out method, while keratin(ST2) by deoxi-dization method.

中文摘要：目的：为了证明蝎皮对免疫功能的影响并探讨蝎皮中含有的活性成分，通过提取分离蝎皮中的水溶蛋白质和角蛋白质组分，经过体外药理活性筛选，观察其对细胞或体液免疫系统的影响，以期获得具有生物活性的蛋白质组分。

In total, 22 differential proteins were identified from 9 fractions.

研究结果表明，利用基于蛋白质水平的在线二维液相色谱分离技术找寻差异蛋白质，具有重现性好、自动化程度高等优点，可用于开展比较蛋白质组学的研究。

For the development of protein drugs and the research of biochemistry, it is significant to probe the interaction between chitosan and protein, and to observe changes of adsorption when both of them contact at interface. Total internal reflection fluorescence spectroscopy is an effective technique to investigate protein adsorption at interface.

壳聚糖对蛋白质有良好的亲和能力，研究蛋白质与壳聚糖在界面上的相互作用、理解蛋白质与壳聚糖分子在界面接触时的吸附过程变化对蛋白药物的开发和生物化学研究具有非常重要的意义。

This suggested there might have cis-acting element in 3'-UTR. In order to search the possible trans-acting factor, we found RNA-protein band in H〓 cells and no band in 95D cells using 3'-UTR probe by RNA EMSA assay. At the same time, this band could be competed with the unlabled probe. There was also no band obsearved using 5'-UTR probe in both cells.

为了寻找可能与3'-UTR结合的蛋白质反式作用因子，用RNA凝胶阻滞(electrophoresis mobility shift assay，EMSA)的方法，我们在H〓细胞的胞浆蛋白质提取物与3'-UTR探针的结合反应中，发现了一条RNA-蛋白质的结合带，而在95D细胞没有结合带出现；而且这种结合带能被未标记探针竞争性抑制；5'-UTR的结合反应也没有观测到结合带的形成。

AOX is a member of di-iron carboxylate proteins. Two structural models exist for AOX and the latest one is a monotopic protein instead of span membrane. Relatively little is known about its 3-dimensional structure. Main reason is that the sufficient quantities of AOX suitable fur crystallogram structural studies is unavailable because of its unstableness.

AOX是首次发现的双铁羧酸蛋白质成员中的膜蛋白质，AOX与膜分离后容易失活，至今尚未有三级结构的报导，只有二级结构的2种假设模式，最新的模式AOX为膜界面蛋白质而不是跨膜蛋白。

In addition, chloroform is venomousness which can descend activity of TPS and enhance deposition of impregnant So, taking into account of cost and entironment, Sevag deprotein is carried through 3 tunes.

利用Sevag脱蛋白，加入1/4体积的氯仿和1/16体积的正丁醇，氯仿使蛋白质变性，变性的蛋白质溶于正丁醇中，而多糖不溶于有机层，则可将蛋白质除去。

These chemical substances have great poison to the body,and easy to suffer belly expanding and stuffy,seeing stars,four limbs of the body acratia,exanimation and this kind of symptom.

一般情况下，蛋白质供应量每人每天每公斤体重1.0～1.5克，占总热量的15%左右，优质蛋白质应占总蛋白质的40%～50%。

In this research, the spider silk from the major ampullate gland of native Nephila pilipes was collected via the forced-silking apparatus at the reeling speed of 3 m/min. The obtained silk was dissolved in hexafluoro-isopropanol. The influence of pH and temperature over the secondary structure of the silk protein in solution state was examined via Circular Dichroism. The effect of silk concentration and electrical field strength on the structure conformation of silk protein during film casting process was investigated via FT-IR. During the drying process, the increase of silk concentration and the presence of electric field tend to form β-sheet and β-turn. Finally, the dried film of silk protein was treated with different temperatures and cation solutions to study the post-treatment effects.

本研究所采用之蜘蛛丝蛋白质系取自本土品种人面蜘蛛之大囊壶腺体，以3 m/min之卷丝速度强迫取丝(forced-silking)收集蜘蛛丝，再将纤维溶於六氟异丙醇溶剂中配制成溶液，以圆二色光谱仪分析在不同 pH 值与温度条件下丝蛋白质在溶液中之二级结构变化，再将蜘蛛丝溶液以不同浓度及电场强度下乾燥成薄膜后，以傅立叶红外线光谱仪检测分析丝蛋白质在固体状态下之二级结构，最后以不同温度加温、离子溶液浸泡等条件对乾燥所得之薄膜进行后处理，并分析其二级结构之变化。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。