蛋白腺

ATP in combination with the muscle proteins actin and myosin lead to the formation of actomyosin, a single phosphate, ADP and the all important energy.

三磷酸腺苷结合肌动蛋白和肌球蛋白导致形成肌动球蛋白，一个单一的磷酸盐， ADP和所有重要的能源。

Objective To identity adenine phosphoribosyltransferases in Schistoma japonicum and analyze their structural features.

目的 鉴定日本血吸虫中存在的腺嘌呤磷酸核糖转移酶，分析其蛋白结构特征。

Objective: To study the relation between EphA2 protein expression and tumorigenesis and development of large intestine adenocarcinoma.

目的 探讨EphA2蛋白的表达与大肠腺癌发生发展的关系。

On the basis of light microscopic observation the immunohistochemical examination of CK,TG,NSE,vimentin and calcitonin was used to observe 9 cases of oxyphilic cell adenoma of the thyroid.

观察9例甲状腺嗜酸细胞腺瘤，在光镜观察的基础上，用免疫组织化学方法检测细胞角蛋白、甲状腺球蛋白、神经元特异性烯醇化酶、波形蛋白和降钙素。

OBJECTIVE: To construct a novel adenoviral eukaryotic expression vector that can co-express mutant hypoxia-inducible factor-1 alpha (HIF-1α) target protein and humanized Renilla reniformis green fluorescent protein reporter molecule under normoxic conditions.

目的：构建能够同时表达突变型低氧诱导因子1α(hypoxia inducible factor 1 alpha，HIF-1α)目的蛋白和人源化绿色荧光蛋白(human renilla reniformis green fluorescent protein，hrGFP)报告分子的新型腺病毒真核细胞表达载体。

Akt,AMPK and ERK proteins were detected by western blot analysis,and the distribution of GLUT4 in these skeletal muscles was observed by immunofluorescence. Results The expression of GLUT4 on the cell membrane was significantly higher in the electrically stimulated groups than in the unstimulated groups.

用Western Blot法测定骨骼肌中蛋白激酶B(protein kinase B，PKB/Akt)、腺苷酸激活的蛋白激酶、细胞外信号调节激酶的表达和活性变化，用免疫荧光方法观察GLUT4在细胞膜及细胞内膜的分布。

One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活，ICAM-1表达升高，与被激活的PMN表面表达的LFA-1相结合，&PMN-EC&相互作用加剧，一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍；另一方面粘附的PMN过度吞噬或激活，当白细胞吞噬的颗粒不能被封闭隔离，连同细胞内的酶被释放出来，其中的PMN-elastase能够降解细胞基质中各种成分，水解多种蛋白，加重胰腺的毛细血管内皮细胞和腺泡的损伤；释放的溶酶体酶使组织蛋白水解，产生的不饱和脂肪酸引发脂质过氧化方应，破坏细胞膜的结构和功能；释放的炎性细胞因子，激发其他的免疫细胞的功能，导致进一步的组织损伤和坏死，加重SAP的病理损伤，最终导致休克、脓毒血症及多器官功能障碍等严重后果。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

METHODS Upon the model of cultured rat FBs, C a2+ influx stimulated with Ang Ⅱ,CaN signaling pathway was blocked with c yclosporine Aand Ca2+ channel with verapamil, detecting the ac tivities of FBs CaN, mitogen activated protein kinase and protein kinas e C,［3H］-leucine(［3H］-Leu) and ［3H］-thymidine(［3H］-Td R) incorporation as the target to evaluate FBs proliferation.

方法以培养的FBs为模型，用Ang Ⅱ剌激FBs外Ca2+入流，环孢素A阻断CaN信号通路，维拉帕米阻断FBs钙通道，检测FBs CaN、丝裂素活化蛋白激酶、蛋白激酶C活性，用［3H］-亮氨酸及［3H］-胸腺嘧啶参入量作为反应FB s增殖的指标。

Studies in neuropsychopharmacology and molecular neurobiology indicate that neurotransmitters and its receptors play important roles in alcohol abuse and addiction, and post-receptor signal transduction pathways, including cyclic adenosine 3', 5'-monophosphate-protein kinase A, phosphoinositide, Ca(superscript 2+)-calmodulin, phospholipase D and tyrosine kinase Fyn signaling cascade.

神经精神药理学和神经分子生物学研究表明，酒精滥用和成瘾不仅与中枢神经系统的神经递质及其相关的受体有关，而且，受体后的信号转导通路参与了酒精依赖的形成过程。其中包括环磷酸腺苷-蛋白激酶A、磷脂酰肌醇、Ca(上标 2+)-钙调蛋白、磷脂酶D以及酪氨酸激酶Fyn等信号途径。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题，你可以随时联系我。