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One hand mechanical obstruct led to the increase of veinous resistance and the obstacle of microcirculation, the other hand the adhesive PMN was activated in excess, the white blood cells released a lot of enzymes, in which PMN-elastase can decompose the components of cell and many albumens, inclusive of immunoglobulin、alexin and fibrication. These components induced the injury of the pancreatic capillary vessels and cell and lysosome enzy made the tissue protein hydrolyze and produced unsaturated fatty acids, which destroyed the structure and function of cellar membrane. The inflammatory cellar factors activate other immunocytes to produce the injury and necrosis of tissue, which aggravated the pathological injury and led to shock、pyaemia and MODS. So ICAM-1 and LFA-1 played an important role in SAP. Frossard found that the expression of ICAM-1 in the rat model, especially in serum、pancreas and lung. All these showed ICAM-1 is an important factor in AP and concomitant lung injury.

胰腺小叶组织局部血管EC首先被激活,ICAM-1表达升高,与被激活的PMN表面表达的LFA-1相结合,"PMN-EC"相互作用加剧,一方面机械性阻塞毛细血管导致静脉阻力增加、微循环障碍;另一方面粘附的PMN过度吞噬或激活,当白细胞吞噬的颗粒不能被封闭隔离,连同细胞内的酶被释放出来,其中的PMN-elastase能够降解细胞基质中各种成分,水解多种蛋白,加重胰腺的毛细血管内皮细胞和腺泡的损伤;释放的溶酶体酶使组织蛋白水解,产生的不饱和脂肪酸引发脂质过氧化方应,破坏细胞膜的结构和功能;释放的炎性细胞因子,激发其他的免疫细胞的功能,导致进一步的组织损伤和坏死,加重SAP的病理损伤,最终导致休克、脓毒血症及多器官功能障碍等严重后果。

The results show that all the hmgr genes from pigs, cattle and chickens are astable enzyme proteins with the same gene sequence characteristics and encoding protein and physical and chemical properties; all the amino acid sequences have a high degree of homology, containing two conservative HMG-CoA binding motifs and two NADPH binding motifs; all the amino acid sequences, containing signal peptide, are secreted proteins; they are all hydrophobic transmembrane proteins with five transmembrane domains; random coil and α-helix are the main structural elements in the HMGR polypeptide chain; all the predictions of three-dimensional structure are V- shaped; there are more closer evolution relationship between the HMGR gene from pigs and cattle.

结果表明:猪、牛和鸡HMGR基因序列特点及编码蛋白质理化性质基本相同,均属不稳定类酶蛋白;保守结构域氨基酸序列具有高度的同源性,均含有保守的2个β-羟基-β-甲基戊二酸单酰辅酶A结合基序和2个还原性烟酰胺腺嘌呤二核苷酸结合基序;均有信号肽,属分泌型蛋白质;属于跨膜的疏水性蛋白,均有5个跨膜结构域;无规则卷曲和α-螺旋是HMGR多肽链中的主要结构元件;三维结构预测都呈"V"字形;猪和牛HMGR基因的进化关系最近。

Immunohistochemistry showed a positive reaction to cytokeratin and a negative reaction with smooth muscle actin..Conclusions:.The Alcian blue negative stain, the negative reaction to SMA, and the fact that the patient is still alive 10 years after exenteration favored the diagnosis of basal cell adenocarcinoma.

免疫组化显示,细胞角蛋白反应阳性,平滑肌肌动蛋白(结论:阿尔辛蓝染色为阴性、SMA反应阴性及病人在眶内容去除术10年后仍然存活的事实都倾向于基底细胞腺癌的诊断。

Result: 1. The situation of HIV infection in gastro-mucosal tissues:(1) Our study found HIV gag sequence in not only CD4~+ T cells but also mucosalepithelial cells, gland epithelial cells in lamina propria and spindle stromal cells;(2) HIV p24 was expressed in T cells, plasmocyte, and a few mucosa epithelial cells, gland epithelial cellls and dell epithelial cellls.

结果:1.HIV在胃黏膜中的感染状况:(1)HIV感染者胃黏膜内HIVgag区基因不仅见于CD4~+T细胞等免疫细胞中,少数胃黏膜上皮、腺上皮、小凹内上皮细胞和间质梭形细胞中亦有阳性杂交信号;(2)HIV感染者尸检胃黏膜内HIVp24蛋白于T细胞、浆细胞及少部分胃黏膜黏膜上皮、腺上皮及小凹上皮细胞中呈阳性表达。2。

Recombinant adenovirus containing major outer membrane protein gene of Chlamydia psittaci of chicken origin were successfully constructed with human adenovirus type 5 vector which lack El and E3 genes.

利用E1和E3缺失的人腺病毒5型载体,首次在国内外成功构建了含有鸡源鹦鹉热衣原体主要外膜蛋白基因的重组腺病毒。

Methods: The method of immunohistochemistry was used to study the expression of CD44v6 protein in the cases, including 10 normal cervix, 15 dysplasia, 16 squamocellular carcinoma in situ , 1 adenocarcinoma in situ , 27 invasive squamocellular carcinoma, and 15 invasive adenocarcinom...

用免疫组化法检测 10例正常宫颈上皮、15例宫颈上皮不典型增生、16例宫颈原位鳞癌、1例宫颈原位腺癌、2 7例宫颈浸润鳞癌、15例宫颈浸润腺癌中CD44v6蛋白的表达,实验结果以图像处理与分析系统进行半定量处理后进行统计分析。

The group of mixture dieted albumin polypeptide with 3H-TdR was compared with the groupdieted only 3H-TdR in mice.

应用同位素示踪法对小鼠小肠、肝及胸腺等组织的3H-胸腺嘧啶核苷(3H-TdR)放射性值进行检测,比较了白蛋白多肽和3H-TdR混合物进食组小鼠与仅进食3H-TdR组小鼠间的区别。

With the methods of Reverse transcription-PCR and cDNA sequencing, the expression of FHIT gene was detected in radiation carcinogenesis. The results show that: 1, The FHIT gene's sequence in normal BALB/c mice compare with the sequence of mice in Ge neBank which absents exon 3, but the function of FHIT protien doesn't alter.

结果发现:1、正常BALB/c 小鼠的FHIT基因序列同GeneBank中小鼠的FHIT基因序列比较缺少了外显子 3,但其表达的蛋白功能并未发生改变;2、FHIT基因在辐射损伤与辐射致癌的早期过程中,对照组血液、胸腺及骨髓均未出现异常的FHIT转录本的表达,不同剂量照射组中血液、胸腺及骨髓均有部分样品出现分子量较小的异常FHIT 转录本的表达。

The organization fixing of apoenzyme subtypes by IHC: The most increasing expressing were cPLA_2 and sPLA_2 in pancreas, they were found all over in the pancreatic acinar cells in 6h after PT, and spread out from the cells in 24h,then decreased after 72h.

用IHC技术对酶蛋白亚型组织定位方面:胰腺内的表达以sPLA_2和cPLA_2增加为主,6h时即遍布于胰腺腺泡细胞;24h时由于胰腺细胞的大量破坏,腺泡细胞外均可见sPLA_2和cPLA_2的高表达;72h后细胞内外sPLA_2和cPLA_2逐渐下降。

Methods Ten mature Wistar rats were divided into normal control group (5 rats) and adenovirus (E1, E3-Deleted and carried math1 and enhanced green fluorescent protein report gene, Ad-Math1-EGFP) scala vestibuli transfer group (5 rats). Right ears of the Ad-Math1-EGFP transfering group rats were deliveried 5μl Ad-Math1-EGFP (physical tite 2.1×10^11v.p./ml) into cochleas through the way of drilling scala vestibuli of cochlear basal turn. As a control, the normal group received nothing to inner ear. In order to estimate functional condition of vestibule and cochlea, the click-evoked potentials on the surface of the cervical dura mater, auditory brain stem response and swimming time were recorded in all rats at 7 days after treatment, and then histologic and morphologic observation were carried out after animals were sacrificed. Results All animals' morphologic observation showed that inner ear hair cells were normal after transfer.

将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein, Ad-Math1-EGFP)前庭阶导入组,每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×10^11v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater, CDM-CEP)、听性脑干反应阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。

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