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蛋白腺

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Objective To study relation of the expression of laminin to the degree of adenoid cystic carcinoma of the salivary gland.

目的 研究层粘连蛋白在涎腺腺样囊性癌中的表达特征及其与腺样囊性癌组织分化程度的关系。

Abstract] objective to study the pathological features and histopathological type and differential diagnosis of hepatic focal nodular hyperplasia.methods the clinicopathological characteristics of 40 cases of fnh were studied.all were evaluted by use of paraffin embedded sections and he staining before light microcope observation.results there were 28 females and 12 males fnh patients whose age were from 16 to 62 years(median 41.3),all alpha-fetoprotein was negative and had no hepatitis history.25 cases were classic type showed characteristic central stellate fibrotic scar,composed of fibrous connestive tissue and tortuous blood vessels.8 cases were telangiectic type,the left were mixed type and adenomatoid type.conclusion fnh is a reactive proliferation of hepatic cells to local blood vessel anomalies,it is not realy a tumor.its differential diagnosis includes hepatic adenomatous hyperplasia nodule,hepatic anaplasia nodular hyperplasia,fibrolamellar hepatocellular carcinoma and hepatocellular adenoma.

目的 探讨肝局灶性结节性增生的病理形态特点、组织分型及鉴别诊断。方法分析40例肝局灶性结节性增生的临床资料,并采用石蜡包埋he染色光镜下观察其组织学特点。结果 40例肝局灶性结节性增生患者中,女28例,男12例,年龄18~62岁,平均年龄41.3岁,所有病例术前均无肝炎病史,甲胎蛋白阴性,组织学上25例为经典型,有特征性的中央纤维瘢痕,由纤维结缔组织及扭曲血管组成。8例为毛细血管扩张型,其余为混合型及腺瘤样增生型。结论肝结节性增生是一种肝细胞对局部血管的异常反应性增生,并非真性肿瘤,主要与肝腺瘤样增生性结节、肝间变性结节状增生、肝纤维板层癌及肝细胞腺瘤鉴别。

Methods: Rat models of chronic renal failure were made by cut 5/6 kindney or fed with feedingstuff which contains adenine(0.75%) for 4 weeks. Experimental treatment was applied by giving Yi Shen Granule and the curative effect of Yi Shen Granule on ongoing pathological changes of CRF was studied. According to the data of CRF rats in weight, water wastage, hemanalysis(RBC、HGB、HCT、MCV、MCH), urinalysis(volume, specific gravity, pH, proteinuria), blood serum analysis(BUN、Cre、ALB、TP), kidney weight coefficient and kidney morphology ( the grade of expansile renal tubule and the diameter of glomerulus ),the pharmacological effect of Yi Shen Granule could be ascertained.

实验选用0.75%腺嘌呤饲喂法致大鼠慢性肾衰模型和5/6肾切除法致大鼠肾衰模型,考察益肾颗粒对慢性肾功能衰竭进行性病变的影响,以造模动物一般状况观察、血液学指标包括红细胞计数、血红蛋白、红细胞压积、平均红细胞体积、平均红细胞血红蛋白(RBC、HGB、HCT、MCV、MCH),尿液指标(尿量、比重SG、pH值、尿蛋白)、血清生化学指标包括尿素氮、肌酐、白蛋白、总蛋白(BUN、Cre、ALB、TP)、脏器解剖和病理观察指标(肾小管扩张分级评分和肾小球径长)做为判定依据。

The high scores of AgNOR and the positive of C-erbB-2 indicates a high degree of malignance of patients with endometrial carcinoma.

AgNOR值增高提示患者可能患有子宫内膜腺癌且提示子宫内膜腺癌恶性程度高,如合并C-erbB-2蛋白的阳性表达率增高可进一步提示患者患有子宫内膜腺癌。

The identified proteins were divided into two following categories: one is regulatory proteins, for example, caffeic acid 3-O-methyltransferas, HSP70, glyoxalase I , actin, and so on. They are involved in protein biosynthesis and processing, cellular structural organization and stress responses. The other is function proteins, for instance, ascorbate peroxidase, cytoplasmic malate dehydrogenase, cytosolic phosphoglycerate kinase, S-adenosyl methionine synthetase, ATP synthase, and so on. They are involved in photosynthesis, photorespiration, redox homeostasis, and metabolisms.

通过分析可以将这些差异蛋白分为两大类:第一类是调节性蛋白,如咖啡酸三氧转甲基酶,热激蛋白70,醛酮变位酶I,肌动蛋白等,它们主要参与蛋白质的合成和加工、胁迫响应、细胞骨架稳定等过程;第二类是功能性蛋白,如抗坏血酸过氧化物酶,细胞质苹果酸脱氢酶,细胞质磷酸甘油酸激酶,S-腺苷蛋氨酸合成酶,ATP合酶等,它们则参与氧化还原平衡、光合作用、光呼吸和各种代谢等过程。

Methods The expression of COX-2 and Survivin of 30 cases of SACC and 20 cases of normal salivary gland was examined with immunohistochemical technique.

采用免疫组织化学检测30例涎腺腺样囊性癌及20例正常涎腺组织中COX-2与Survivin蛋白的表达情况。

The construction of recombinant UNPase provided a basis for the research on biocatalysed synthesis of adenosine. In comparison with uridine, inosine was the better donar of pentose-1-phosphorate. In the reaction mixture composed of 30 mmolL^(-1)inosine, 10 mmolL^(-1) adenine, and 30 mmolL^(-1) pH 7.5 phosphate buffer and 0.5% wet cells gave a high yield of 85.57% at 60℃ in 3h.

在此基础上研究了不同诱导条件对蛋白表达量的影响分别以肌苷和尿苷为核糖供体研究重组菌转化腺苷的条件,发现肌苷最适合作为核糖供体,在30mmolL^(-1)pH7.5的Na2HPO4-KH2PO4缓冲液中,以30mmolL肌苷和10mmolL^(-1)腺漂呤作底物,加入0.5%基因工程湿菌体60℃反应3h,腺苷的转化率为85.57%。

The thymuses, spleens, sera and anticoagulant blood were collected after the rats were killed, to carry out these works:(1) Zinc concentrations were analyzed by atomic absorption spectrophotometry;(2) Body weight and the weight of thymuses and spleens were measured, the organ index were calculated;(3) Changes in pathology of thymus and spleen were observed;(4) Using TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling) method, the apoptosis of thmocytes and spleen lymphocytes was checked;(5) The expression of bcl-2、bax mRNA in thymus and spleen were detected by RT-PCR (reverse transcription polymerase chain reaction);(6) The expression of p56〓, a signal transduction protein in thymus and spleen were detected by immunohistochemistry;(7) Two-color cytofluorometric analysis was used to assess the expression of CD4, CD8, CD45RA and CD45RC in peripheral blood lymphocytes.

动物处死后留组织、抗凝血及血清,进行以下检测:(1)原子吸收法测血清锌浓度;(2)测胸腺、脾脏脏器重量并与大鼠体重比较,计算脏器指数;(3)HE染色对胸腺、脾脏进行病理观察;(4)原位末端标记法测胸腺、脾脏中淋巴细胞的自发凋亡,并通过图像分析进行比较;(5)逆转录聚合酶链式反应检测凋亡调控基因bcl-2、bax mRNA在免疫器官中的表达;(6)免疫组化方法检测信号转导蛋白p56〓在免疫器官中的表达;(7)流式细胞术分析外周血淋巴细胞中CD4+、CD8+、CD45RA+及CD45RC+CD4+、CD45RC-CD4+细胞的数量和比例。

Methods samples from 78 patients with pituitary adenomas, including 37 invasive and 41 noninvasive, and 5 normal pituitary tissue specimens from autopsied patients were examined. the expressions of opn mrna and opn were examined by real-time fluorescence quantitative pcr and immunohistochemical sp method, respectively. the relation of opn and opn mrna expressions to invasiveness of pituitary adenomas was analyzed.

分别对78例垂体腺瘤手术标本(其中侵袭性37例,非侵袭性41例)和5例来源于尸检的正常垂体组织进行检测,采用实时荧光pcr技术检测组织中opn mrna表达,免疫组织化学sp法检测opn蛋白表达,结合临床资料分析opn mrna及其蛋白的表达与垂体腺瘤侵袭性的关系。

There was no difference between breast cancer and fibro adenoma tissues in ERαexpression.The expression of ERαin breast cancer tissues was not associated with histological types,age,clinical stage,lymph node metastases state,HER-2 and P53 protein expression.

结论1、乳腺癌组织ERα蛋白表达与乳腺纤维腺瘤组织比较无显著差异;乳腺癌组织中ERα蛋白表达与患者年龄、病理类型、临床分期、淋巴结转移、P53、HER-2蛋白无关,与PR蛋白表达正相关。

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