蛋白腺

Using SMARTTM cDNA library construction technology from Clontech biocompany,we have constructed 4 Matchmaker cDNA libraries for posterior silk gland of 4th moulting larvae,posterior silk gland,middle silk gland and fat body of larvae at 3rd day of 5th instar, respectively.

家蚕的丝腺是一个特异化的器官，主要用于合成蚕丝蛋白，包括在家蚕的后部丝腺表达的丝素重链蛋白、丝素轻链蛋白、P25蛋白(fibrohexamerin，Fhx)以及在家蚕中部丝腺表达的丝胶蛋白。

The adenovirus plasmid was identified by PacI digestion and transfected into 293A cells to package a recombinant adenovirus which expressed the Fhit protein. Furthermore, the adenovirus rAd-Fhit was infected into colon cancer cells,and the expression of the ectogenic protein was detected by Western blotting. Finally, the proliferation of colon cancer cells was observed in adenovirus-infected cells by the MTT assay.

经PacI酶切鉴定正确后，将重组腺病毒质粒转染293A细胞获得表达Fhit蛋白的重组腺病毒rAd-Fhit，将获得的重组腺病毒感染结肠癌细胞，采用蛋白印迹法检测外源Fhit蛋白的表达，并进一步观察其对细胞增殖能力的影响。

Struma Cuvier consists of three parts: digenetic gland, the reproductive ducts and subsidiary glands of male, the reproductive ducts and subsidiary glands of female.

雄性生殖管道由输精管、阴茎、刺激器组成，附性腺为主要的附属腺体；雌性生殖管道由输卵管、受精囊、阴道组成，附属腺体主要为卵黄腺和蛋白腺。

In the normal uterus, Cytokeratins immunolabelling were detected in glandular cell, luminal epithelial cell, Vimentin immunolabelling were detected in stromal cell and endoblastic cell; CK7 immunolabelling were not detected in any tissue of the yak utenus.

研究结果显示：未妊娠时，泛角蛋白在子宫内膜腺上皮细胞、腔上皮细胞内表达，波形蛋白在子宫内膜基质细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，牦牛子宫任何部位均不表达角蛋白7；妊娠30天左右时，泛角蛋白在子宫内膜腺上皮细胞、子宫内膜腔上皮细胞、滋养层细胞、内胚层细胞和尿囊细胞内表达，波形蛋白在子宫内膜基质细胞和内胚层细胞内表达，平滑肌肌动蛋白在子宫平滑肌和血管平滑肌内表达，角蛋白7在尿囊细胞内表达，偶尔在腔上皮细胞的细胞核边缘表达；消化法进行原代培养时，组织经胶原酶消化并通过100目和400目筛网组合可以有效地分离原代子宫内膜基质细胞和子宫内膜腺上皮细胞；分离得到的子宫内膜基质细胞活率达90%以上，并可在体外传代7次以上；分离得到的子宫内膜腺上皮细胞活率可达85%以上，并可在体外传代5次以上；RPMI1640培养基最适合子宫内膜基质细胞和子宫内膜腺上皮细胞的生长，维持子宫内膜基质细胞正常生长的FBS添加量为20%，维持子宫内膜腺上皮细胞正常生长的胎牛血清添加量为30%。

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma, 16/32 cases of squamous cell carcinoma, 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见P53蛋白表达，而中、重度不典型增生，原位癌，浸润癌中，P53蛋白阳性表达率逐渐增高，其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma，BCC)、16/32例鳞状细胞癌（squamous cell carcinoma，SCC）、10/17例睑板腺癌中，P53蛋白阳性表达；同时，随着肿瘤组织分化程度的降低，P53蛋白阳性表达程度有逐渐增高的趋势。

Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index progressively increased too.

结果 在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见 P53蛋白表达，而中、重度不典型增生，原位癌，浸润癌中， P53蛋白阳性表达率逐渐增高，其 PCNA指数也有增加趋势。30/53例基底细胞癌(basal cell carcinoma，BCC)、16/32例鳞状细胞癌（squamous cell carcinoma，SCC）、10/17例睑板腺癌中， P53蛋白阳性表达；同时，随着肿瘤组织分化程度的降低， P53蛋白阳性表达程度有逐渐增高的趋势。

The positve rates in serous carcinomas and borderline tumors were 39.3% and 55.6%, significantly lower than that in benign tumors and normal ovaries (P.05), but the statistical differences were not found between borderline tumors and serous carcinomas, benign tumors and normal ovaries.2 WWOX mRNA and protein expression in ovarin seruous tumors2.1 WWOX mRNA expression in ovarin seruous tumors The result of RT-PCR showed that WWOX mRNA expression were detected in 29 of 32 ovarian serous benign tumors, 12 of 16 ovarian serous borderline tumors, and 21 of 45 ovarian serous carcinomas respectively.

阳性条带密度扫描定量结果，卵巢浆液性癌中PTEN蛋白表达相对量为0.34±0.12比正常卵巢和浆液性囊腺瘤和交界性囊腺瘤明显降低(p.05)，其余各组间无统计学差异。3.2.2 PTEN蛋白FCM检测结果FCM结果，卵巢浆液性癌PTEN蛋白表达的FI值(0.908±0.023)明显低于正常卵巢、卵巢囊腺瘤和交界性囊腺瘤的FI值，差异有统计学意义(P.05)。3.2.3 PTEN蛋白免疫组织化学结果35例浆液性囊腺瘤、18例交界性囊腺瘤和56例浆液性癌中，分别有31例、15例和29例呈阳性表达。

The reproductive duct of the female consisted of the oviduct, receptaculum seminis, and vagina, and the main subsidiary glands were the viteline gland and albumen gland.

雄性生殖管道由输精管、阴茎、刺激器组成，前列腺为主要的附属腺体；雌性生殖管道由输卵管、受精囊、阴道组成，附属腺体主要为卵黄腺和蛋白腺。

The shorter variant mHemk2 is 1696 bp in length encoding a putative mouse protein of 14.7 kDa, consisting of 138 amino acid residues.In protein data bank annotations, Hemk has previously been classed among methyltransferases methylating N6-adenine or N4-cytidine in DNA because of the presence of S-adenosyl-L-methionine binding motif and of an NPPY motif in the protein.

在蛋白数据库的注释中，由于在Hemk蛋白中存在S-腺苷-L-甲硫氨酸结合基序和一个NPPY基序，因此，在蛋白库的说明中已经将Hemk蛋白归类于能甲基化DNA中N6-腺嘌呤或N4-胞嘧啶的甲基转移酶。

Five expressed genes contributed to the flowering process and embryonic development such as prolamine, gene for allergenic protein, chalcone synthase, putative peptide methionine sulfoxide reductase and acyl carrier protein Ⅱ gene were chosen to hybridize with a 1510-RNA-blot (time-point phenotypes) array. The 1510 RNA blots were prepared into an RNA array for the purpose of conferring and validating global transcriptional profiles on booting, flowering and filling quantitatively and qualitatively at a statistic level. The results verified: Prolamine and allergenic protein were high-expressed in filling grains after being regulated by development, Chalcone synthase and methionine sulfoxide reductase were high-expressed in the leavies induced by light, in the root stressed for nitrogen deficiency.

为了全局性分析基因表达谱以及进一步验证它们与开花过程的相关性，选取参与水稻开花过程和胚胎发育过程的基因：查尔酮合酶、酰基载体蛋白Ⅱ、醇溶蛋白、S-腺苷基甲硫氨酸还原酶、过敏反应蛋白等为探针，与1500个水稻RNA斑点阵列进行杂交时，结果证实：醇溶蛋白和过敏反应蛋白受发育调节在乳熟成穗中高表达，查尔酮合酶和S-腺苷基甲硫氨酸还原酶在叶片中受光诱导、在根中受缺氮胁迫高表达。

In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国，慢性酒精中毒，肝炎是最常见的。

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如果有任何问题，你可以随时联系我。