蛋白的

To study the cytopathogenic effect of mycoplasma membrane.the pro- toplasmic membrane of the mycoplasma causing swine enzootic pneumo- nia was prepared by the repeated Freeze-thaw method or the osmotic shock method,and was then inoculated into the rabbit kidney primary tissue cultures for examining their biological activities.The experimental results were as follows:in the cultures inoculated with 25～50μg of membrane protein ,the cytopathogenic effect was considerable.in the cul- tures inoculated with ...

用低渗解体及反复冻融裂解的方法，制备了猪喘气病病原霉形体膜，将所制备的膜制品接种兔肾原代单层细胞，作生物学活性观察，在接种量按蛋白含量计算为25～50微克膜蛋白的细胞管中观察致细胞病变作用，在接种17.5微克膜蛋白的细胞管中作用不明显，接种6微克膜蛋白的细胞管无致细胞病变作用。

Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面，作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况，但gfp本身是个相当大的蛋白，会降低目的蛋白的表达；另一方面，进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀，既不经济又不方便。

Change of expression of AQP4 protein was the same like the change of expression of AQP4 mRNA. We observed it in model group was statisticsly higher than that of control group. The quantity of AQP4 protein in intervenor groups was remarkably lower than that in model group.But the quantity of AQP4 protein in intervenor groups obviously increased compared with control group.

5AQP4蛋白表达与AQP4 mRNA表达呈现相同变化趋势，与对照组相比较，模型组各时间点的AQP4蛋白的含量均显著升高，以造模后第24h最明显；AQP4干预组和HO-1干预组在各时间点的AQP4蛋白的含量均显著高于对照组，但明显低于模型组AQP4蛋白的含量。

Now the mechanism of action of heat shock protein is not quite sure to us.Understanding the biological function and its transplantation immunity of HSP will be helpful in investigating the relationship of HSP and graft ischemical reperfusion injury,graft rejection,HSP gene transfer,cardiac graft vascular disease.

目前，还不能确定热休克蛋白在上述反应过程中的直接作用的机制，了解热休克蛋白的生物学功能及其在移植免疫中的作用，有助于进行热休克蛋白与缺血/再灌注、移植排斥反应，以及热休克蛋白转基因治疗移植物血管病关系的研究。

Results］ the expression of cox-2 in hepatocellular carcinoma and its adjacent tissues with cirrhosis were 70.0% and 92.0% respectively, and they were higher than that in normal liver tissue (20.0%). the cox-2 expression in carcinoma adjacent tissues with cirrhosis was higher than that in hcc. expression of cox-2 in well differential hcc was higher than that in poor differential hcc, and higher in case with metastasis than that without metastasis(p＜0.01).the expression of cox-2 in hcc did not relate to tumor size, tumor capsula and the level of afp.

结果］ cox-2蛋白在hcc、癌旁肝硬化中表达阳性率分别为70.0%和92.0%，显著高于在正常肝组织中的20.0%（p＜0.05），而cox-2在癌旁肝硬化组织中的表达高于hcc组织。cox-2蛋白在分化好hcc中的表达显著高于分化差的hcc（p＜0.05），转移组中cox-2蛋白表达显著高于无转移组（p＜0.01）；而cox-2蛋白的表达与肿瘤大小、有无包膜及afp水平无关。

Genome, and its expression products are located in the flagellar, cytoplasm and fertilization tubule. Cr. actin is partially purified from Cr. cells by the method of assembly-disassembly-circle of actin.

我们利用肌动蛋白本身所具有的聚合和解聚的性质，从衣藻细胞中部分纯化了衣藻肌动蛋白，纯化的肌动蛋白能和抗兔α-平滑肌肌动蛋白的单克隆抗体发生专一性很强的免疫交叉反应。

The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

A potential approach to this problem is to make use of a natural defense mechanism called " the stress response," which is characterized by a transient down - regulated synthesis of nonvital cellular proteins and increased production of endogenous protective factors known as heat shock proteins that act to allow proper refolding of proteins denatured by the incipient injury.

应激反应的特征是一过性下调细胞非必需蛋白的合成及增加被称为热休克蛋白的内源性保护因子的合成。热休克蛋白作用是促使损伤导致变性的蛋白适当地再折叠。HSP家族中HSP70被认为在细胞保护中发挥重要作用。

The coagulating effect of the two proteinases and their mixtureon 7S and 11S was compared. Results indicated that two proteinases interacted witheach other during the coagulating process. 11S gel induced by mixture of twoproteinases was more elastic, and 7S gel induced by separated proteinase was moreelastic.

然后采用SP-sepharose阳离子交换剂从木瓜蛋白酶分离出2种蛋白酶组分，对比它们单独作用和复合作用于7S和11S蛋白的促凝能力，结果表明木瓜蛋白酶中两种组分对7S和11S蛋白的促凝过程中存在协同作用，复合作用可以促使11S蛋白形成弹性更强的凝胶，而单独作用可以促使7S蛋白形成弹性更强的凝胶。

I. Design and selection of thc high affinity DNA ligands for singIe-chain reprcssorSingle-chain repressor RRn., is a derivative of bacteriophage 434 repressor,which contains covalent1y dimerized DNA-binding domains (axnino acids 1-69) of thephage 434 repressor.

一。设计和筛选单链阻遏蛋白的高亲和力DNA结合序列单链阻遏蛋白RR_是噬菌体434阻遏蛋白的衍生物，它是噬菌体434阻遏蛋白的N端DBD（1—69位氨基酸）组成的共价二聚体。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。