蛋白的

The results show:(1) In testis, PNA receptors sites were firstly observed in the cytoplasm and plasma membrane of primary spermatocyte, and greatly increased in the plasma membrane of second spermatocyte, then decreased and were accumulated in the tail of the round spermatid. The same reaction was observed in sperms of testis and spermatheca, which shows stronger reaction in head and weaker in tail. A universal dyeing was observed on the plasma membrane of sertoli cells.

结果表明：(1)在中华蚱蜢的精子发生中，PNA受体是由初级精母细胞合成，然后转移到膜上，在次级精母细胞，由于量的积累其质膜出现了强阳性标记；从早期精子细胞到成熟精子时期糖蛋白的分布发生了明显的修饰变化，这些变化与精子获得与卵子进行识别、粘附、结合等受精能力密切相关：受精囊内与精巢内的精子表面的标记是相同的，说明质膜糖蛋白的修饰作用在精巢内就已完成。

"While preious research has demonstrated that arious types of stem cells can turn into cells that express the proteins consistent with smooth muscle, this is the first study that shows that the cells we generated hae the same functional properties as smooth muscle, as well as express the same proteins," said Jeffrey Ross, Ph.D., research associate at the Stem Cell Institute.

"以前的研究已经证实多种不同类型的干细胞可以被转化成表达与平滑肌细胞相同蛋白的细胞，这个实验首次显示，我们得到的细胞由于平滑肌相同的特性，表达一样的蛋白，"干细胞研究所的研究员Jeffrey Ross博士说。

When EGTA (Ca〓 chelator), Verapmil and LaCl〓(Ca〓 channel blockers) were used to treat tomato fruit at green mature and strawberry fruit at white stage with ethylene, they could reverse ethylene-induced increase in ethylene production in tomato and strawberry, PG activity, lycopene content, soluble protein content in cell wall in tomato and degradation in soluble protein in strawberry, which indicated blocking Ca〓 channel in plasma membrane or chelating Ca〓 can decrease intracellular Ca〓, further inhibite ethylene-induced maturation, ripening and senescence of fruit.

用质膜钙通道阻断剂异博定、钙通道Ca〓竞争性抑制剂以及Ca〓专一性螯合剂与乙烯一起处理绿熟期番茄果实和乳白期草莓果实，均可抑制乙烯诱导番茄和草莓的乙烯生成、番茄PG活性的提高、番茄红素含量的增加和细胞壁可溶性蛋白含量的增加以及草莓可溶性蛋白的分解，表明阻断质膜Ca〓通道或螯合胞外Ca〓以减少胞内Ca〓能抑制乙烯对果实的催熟作用，间接证明乙烯对果实成熟衰老的诱导与胞外Ca〓内流引起的胞质Ca〓浓度的增加密切相关，表明乙烯信号转导可能与钙信使有关，调节胞质Ca〓浓度可调节乙烯对果实的催熟作用。

The result of whole mount in situ hybridization showed that MmeFer was located at the position of shell initiation in trochophore stage, indicating MmeFer plays a role in shell initiation in M. meretrix. The full-length of M. meretrix cathepsin B cDNA was cloned with 3'and 5'RACE. The temporal and spatial expressions of MmeCB mRNA were examined from trochophore to post larva stages by whole mount in situ hybridization. From L2 to L4 stages, MmeCB mRNA was detected in the digestive tract, suggesting a possible role of MmeCB in digestion.

根据构建的文蛤幼虫cDNA文库中提供的序列信息，从文蛤中克隆了与铁离子代谢密切相关的铁蛋白的全长cDNA序列；通过Real time PCR发现，MmeFer mRNA的表达量在贝壳形成前后有明显改变；整体原位杂交结果显示MmeFer mRNA在L1期的表达部位刚好是贝壳生成的起始部位，推断文蛤铁蛋白与文蛤幼虫贝壳初始形成密切相关。

As with rhIL-1β, rhTNFα(100ng/ ml) can also provoked ICAM-1 surface expression by HMC. We also found VCAM-1 mRNA expression by HMC in normal culture condition and exposure of mesangial cells to rhTNFαis associated with increased VCAM-1 mRNA lavels rapidly (4 hours).These exploration indicate (1) glomerular and tubular ICAM-1 expression level correlate with inflammatory degree of glomerulus and TNF-αexpression level (2) VCAM-1 may have certain role in patients of lupus nephritis and crescentic nephritis (3) Expression of ICAM-1 and VCAM-1 may be regulated in vivo by cytokines (TNFα and IL-1β).

与轻度系膜增生性肾炎病人血清中的VCAM-1水平比较狼疮肾炎病人血清中VCAM-1的水平显著增加；（3）rhIL-1β（25ng/ml）不但能增加ICAM-1 mRNA的表达，还增加ICAM-1蛋白的表达；rhTNFα（100ng／ml）对ICAM-1蛋白表达和VCAM-1 mRNA基因表达均有明显的上调作用，结果提示：肾小球和肾小管ICAM-1表达水平与肾小球炎症病变程度有关；VCAM-1在狼疮肾炎和新月体肾炎的发生发展中可能有重要作用；体内ICAM-1和VCAM-1的表达可能受TNFα和IL-1β的调节。

To investigate the expression, location and function, long distance PCR was used to expand the targets containing open reading frame. The expressive vectors of prokaryotic and eukaryotic cells were constructed after product purification and connecting with vectors. The prokayotic vectors were induced to express the protein by IPTG and the protein was seen by denaturalization PAG electrophoresis and dyeing. The eukayotic expressive vectors were transfected into culture cells and the protein was found in the nulceolus under the observation of the confocal microscope. The transfected cells were chosen by the geneticin (G418). The cell cyele was examined by flow cytometer and the cell numbers were reduced obviously in the stage of G〓-G〓 and G〓-M, but increased distinguishably in the S stage.

为研究磷酸化应激诱导蛋白的表达、定位和功能，采用了长距离聚合酶链反应扩增含有开放阅读框架的靶片段，经产物纯化、与载体连接，分别构建了STIP1基因的原核表达载体和真核表达载体；IPTG诱导原核表达载体，变性聚丙酰胺凝胶电泳与染色后，可见相应大小的蛋白质表达；STIP1基因的真核表达载体转染细胞系后，共聚焦显微镜下观察STIP1蛋白主要分布于细胞核内；用药物筛选STIP1基因转染的细胞后，流式细胞仪检测细胞周期，可见G〓-G〓期和G〓-M期的细胞明显降低，S期细胞明显增多。

The 3D structure of N protein shows that the domain of Ep703 is exposed on the surface of the protein, making the epitope to be readily recognized by antibody. No homology is found between the mimotopes and native epitope. However, they all contain high content of hydrophile amino acids and low content of hydrophobile amino acids.

蛋白的三维结构显示，Ep703的9个氨基酸多肽暴露于蛋白的表面。3个模拟表位与表位Ep703的序列&IQTAFNQGA&相比，相同的氨基酸很少，但是各表位氨基酸的极性却有一个共同的特征：亲水性氨基酸比例较大，疏水性氨基酸比例较少。

The effects of four factors (concentration and the quantity of NaOH,temperature and heating time) on the protein recovery were studied on the base of the analysis of mycelium residue of itaconic acid fermentation.

研究了衣康酸工业化生产中所产生的土曲霉废菌丝体的主要组成成分，用碱液提取的方法进行了蛋白质的提取回收实验，考察了NaOH溶液浓度、用量、加热温度及时间对菌丝体脱蛋白效果的影响，结果表明衣康酸菌体脱蛋白的最优条件为：NaOH液浓度0.5mol/L，碱液用量为菌丝体的40倍，90℃条件下加热9h。

Two relevant sites of enzymatic digestion were added to the mTNF-α by PCR. The mTNF-α was linked to the 3'end of m/〓 in pGEX4T-1 vector. The prokaryotic expression vector pGEX4T-1m/〓-mTNF-α was constructed successfully. After induction and expression by IPTG, the expression of two kinds of fusion protein is 15% and 12% of total bacteria proteins respectively. The anti-HCC bifunctional antibodies m/〓-mTNF-α were identified by electrophoresis after the inclusion bodies were purified, denature, renature, re-purified, digested by thrombin and further purified.

采用PCR的方法在mTNF-α的两端加上所需要的酶切位点，将之连接在m／〓的3'端，构建原核表达载体pGEX4T-1 m／〓-mTNF-α，通过IPTG的诱导表达之后，两种融合蛋白的表达量分别占细菌总蛋白的15％、12％，表达产物经包涵体的纯化→变性→复性→纯化→凝血酶酶切→进一步纯化后，可以得到纯度为电泳纯的m／〓-mTNF-α抗肝癌双功能抗体。

The FosB/△FosB positive staining was detected in nucleus accumbens septum, tuberculum olfactorium and dorsal caudate putamen, without little cFos positive staining. The results indicate the expression of cFos and FosB/△FosB display the differences in area and intensity, and the area intensively expressing FosB/△FosB is more nearly coincide with the area related to schizophrenia. So FosB/△FosB is a better mark to research the pathogenesis of schizophrenia.

同时大量研究证明，动物模型的行为及生理变化与中枢神经系统神经元的病理变化有关，因此，利用即早基因反应的灵敏性、功能多样性、检测方便性等，应用免疫组织化学的方法对模型小鼠脑内cFos和FosB/△FosB蛋白的表达进行检测，通过确定不同脑区cFos和FosB/△FosB蛋白的表达，来研究哪些脑区或神经元参与了反应，从而发现精神分裂症在脑区内所涉及的解剖结构，有助于对精神分裂症发病机制的认识。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。